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Pharmacological characterization and gene expression profiling of an L5/L6 spinal nerve ligation model for neuropathic pain in mice.
Neuroscience. 2008 May 02; 153(2):492-500.N

Abstract

L5/L6 spinal nerve ligation (SNL) in rodents induces behavioral signs similar to the symptoms of neuropathic pain in humans. L5/L6 SNL in rats has been well characterized so far, but there have been few studies using mice. In this study, we established an L5/L6 SNL model in mice and examined the effects of known antinociceptive drugs in the model. We also analyzed the changes in gene expression in dorsal root ganglions with special reference to those which are known to change in a neuropathic pain state to validate the model. Mechanical allodynia in the ipsilateral side paw was observed beginning on day 1 and lasted for at least 2 months following surgery. Diclofenac showed no significant effect on the mechanical allodynia. Gabapentin and pregabalin completely reversed allodynia, but they also caused a decrease in locomotor activity. Duloxetine caused a partial recovery of the threshold. Mexiletine completely reversed allodynia, but it also caused sedation or motor impairment. Morphine caused a partial recovery of the threshold and hyper-locomotion. This mouse L5/L6 SNL model represents a robust mechanical allodynia, which shows a similar pharmacological response to that reported in rats and human patients with neuropathic pain. The pattern changes in gene expression also resembled those reported in rats. This model will therefore be useful for investigation of the effects of novel antinociceptive compounds and the mechanisms of neuropathic pain.

Authors+Show Affiliations

Pharmacology Research Labs, Drug Discovery Research, Astellas Pharma Inc., 21 Miyukigaoka, Tsukuba-shi, Ibaraki, Japan. tetsuo-kiso@jp.astellas.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18400411

Citation

Kiso, T, et al. "Pharmacological Characterization and Gene Expression Profiling of an L5/L6 Spinal Nerve Ligation Model for Neuropathic Pain in Mice." Neuroscience, vol. 153, no. 2, 2008, pp. 492-500.
Kiso T, Watabiki T, Tsukamoto M, et al. Pharmacological characterization and gene expression profiling of an L5/L6 spinal nerve ligation model for neuropathic pain in mice. Neuroscience. 2008;153(2):492-500.
Kiso, T., Watabiki, T., Tsukamoto, M., Okabe, M., Kagami, M., Nishimura, K., Aoki, T., & Matsuoka, N. (2008). Pharmacological characterization and gene expression profiling of an L5/L6 spinal nerve ligation model for neuropathic pain in mice. Neuroscience, 153(2), 492-500. https://doi.org/10.1016/j.neuroscience.2008.02.031
Kiso T, et al. Pharmacological Characterization and Gene Expression Profiling of an L5/L6 Spinal Nerve Ligation Model for Neuropathic Pain in Mice. Neuroscience. 2008 May 2;153(2):492-500. PubMed PMID: 18400411.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pharmacological characterization and gene expression profiling of an L5/L6 spinal nerve ligation model for neuropathic pain in mice. AU - Kiso,T, AU - Watabiki,T, AU - Tsukamoto,M, AU - Okabe,M, AU - Kagami,M, AU - Nishimura,K, AU - Aoki,T, AU - Matsuoka,N, Y1 - 2008/02/29/ PY - 2008/01/07/received PY - 2008/02/15/revised PY - 2008/02/19/accepted PY - 2008/4/11/pubmed PY - 2009/6/30/medline PY - 2008/4/11/entrez SP - 492 EP - 500 JF - Neuroscience JO - Neuroscience VL - 153 IS - 2 N2 - L5/L6 spinal nerve ligation (SNL) in rodents induces behavioral signs similar to the symptoms of neuropathic pain in humans. L5/L6 SNL in rats has been well characterized so far, but there have been few studies using mice. In this study, we established an L5/L6 SNL model in mice and examined the effects of known antinociceptive drugs in the model. We also analyzed the changes in gene expression in dorsal root ganglions with special reference to those which are known to change in a neuropathic pain state to validate the model. Mechanical allodynia in the ipsilateral side paw was observed beginning on day 1 and lasted for at least 2 months following surgery. Diclofenac showed no significant effect on the mechanical allodynia. Gabapentin and pregabalin completely reversed allodynia, but they also caused a decrease in locomotor activity. Duloxetine caused a partial recovery of the threshold. Mexiletine completely reversed allodynia, but it also caused sedation or motor impairment. Morphine caused a partial recovery of the threshold and hyper-locomotion. This mouse L5/L6 SNL model represents a robust mechanical allodynia, which shows a similar pharmacological response to that reported in rats and human patients with neuropathic pain. The pattern changes in gene expression also resembled those reported in rats. This model will therefore be useful for investigation of the effects of novel antinociceptive compounds and the mechanisms of neuropathic pain. SN - 1873-7544 UR - https://www.unboundmedicine.com/medline/citation/18400411/Pharmacological_characterization_and_gene_expression_profiling_of_an_L5/L6_spinal_nerve_ligation_model_for_neuropathic_pain_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0306-4522(08)00309-6 DB - PRIME DP - Unbound Medicine ER -