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High throughput kinetic Vibrio fischeri bioluminescence inhibition assay for study of toxic effects of nanoparticles.
Toxicol In Vitro. 2008 Aug; 22(5):1412-7.TV

Abstract

Despite of the growing production and use of nanoparticles (NPs) in various applications, current regulations, including EC new chemical policy REACH, fail to address the environmental, health, and safety risks posed by NPs. This paper shows that kinetic Vibrio fischeri luminescence inhibition test--Flash Assay--that up to now was mainly used for toxicity analysis of solid and colored environmental samples (e.g. sediments, soil suspensions), is a powerful tool for screening the toxic properties of NPs. To demonstrate that Flash Assay (initially designed for a tube luminometer) can also be adapted to a microplate format for high throughput toxicity screening of NPs, altogether 11 chemicals were comparatively analyzed. The studied chemicals included bulk and nanosized CuO and ZnO, polyethylenimine (PEI) and polyamidoamine dendrimer generations 2 and 5 (PAMAM G2 and G5). The results showed that EC50 values of 30-min Flash Assay in tube and microplate formats were practically similar and correlated very well (log-logR2=0.98), classifying all analyzed chemicals, except nano CuO (that was more toxic in cuvette format), analogously when compared to the risk phrases of the EC Directive 93/67/EEC for ranking toxicity of chemicals for aquatic organisms. The 30-min EC50 values of nanoscale organic cationic polymers (PEI and dendrimers) ranged from 215 to 775 mg/l. Thirty-minute EC50 values of metal oxides varied largely, ranging from approximately 4 mg/l (bulk and nano ZnO) to approximately 100 mg/l (nano CuO) and approximately 4000 mg/l (bulk CuO). Thus, considering an excellent correlation between both formats, 96-well microplate Flash Assay can be successfully used for high throughput evaluation of harmful properties of chemicals (including organic and inorganic NPs) to bacteria.

Authors+Show Affiliations

Laboratory of Molecular Genetics, National Institute of Chemical Physics and Biophysics, Akadeemia tee 23, Tallinn, Estonia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

18400463

Citation

Mortimer, M, et al. "High Throughput Kinetic Vibrio Fischeri Bioluminescence Inhibition Assay for Study of Toxic Effects of Nanoparticles." Toxicology in Vitro : an International Journal Published in Association With BIBRA, vol. 22, no. 5, 2008, pp. 1412-7.
Mortimer M, Kasemets K, Heinlaan M, et al. High throughput kinetic Vibrio fischeri bioluminescence inhibition assay for study of toxic effects of nanoparticles. Toxicol In Vitro. 2008;22(5):1412-7.
Mortimer, M., Kasemets, K., Heinlaan, M., Kurvet, I., & Kahru, A. (2008). High throughput kinetic Vibrio fischeri bioluminescence inhibition assay for study of toxic effects of nanoparticles. Toxicology in Vitro : an International Journal Published in Association With BIBRA, 22(5), 1412-7. https://doi.org/10.1016/j.tiv.2008.02.011
Mortimer M, et al. High Throughput Kinetic Vibrio Fischeri Bioluminescence Inhibition Assay for Study of Toxic Effects of Nanoparticles. Toxicol In Vitro. 2008;22(5):1412-7. PubMed PMID: 18400463.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High throughput kinetic Vibrio fischeri bioluminescence inhibition assay for study of toxic effects of nanoparticles. AU - Mortimer,M, AU - Kasemets,K, AU - Heinlaan,M, AU - Kurvet,I, AU - Kahru,A, Y1 - 2008/02/26/ PY - 2007/11/05/received PY - 2008/02/15/revised PY - 2008/02/18/accepted PY - 2008/4/11/pubmed PY - 2008/8/30/medline PY - 2008/4/11/entrez SP - 1412 EP - 7 JF - Toxicology in vitro : an international journal published in association with BIBRA JO - Toxicol In Vitro VL - 22 IS - 5 N2 - Despite of the growing production and use of nanoparticles (NPs) in various applications, current regulations, including EC new chemical policy REACH, fail to address the environmental, health, and safety risks posed by NPs. This paper shows that kinetic Vibrio fischeri luminescence inhibition test--Flash Assay--that up to now was mainly used for toxicity analysis of solid and colored environmental samples (e.g. sediments, soil suspensions), is a powerful tool for screening the toxic properties of NPs. To demonstrate that Flash Assay (initially designed for a tube luminometer) can also be adapted to a microplate format for high throughput toxicity screening of NPs, altogether 11 chemicals were comparatively analyzed. The studied chemicals included bulk and nanosized CuO and ZnO, polyethylenimine (PEI) and polyamidoamine dendrimer generations 2 and 5 (PAMAM G2 and G5). The results showed that EC50 values of 30-min Flash Assay in tube and microplate formats were practically similar and correlated very well (log-logR2=0.98), classifying all analyzed chemicals, except nano CuO (that was more toxic in cuvette format), analogously when compared to the risk phrases of the EC Directive 93/67/EEC for ranking toxicity of chemicals for aquatic organisms. The 30-min EC50 values of nanoscale organic cationic polymers (PEI and dendrimers) ranged from 215 to 775 mg/l. Thirty-minute EC50 values of metal oxides varied largely, ranging from approximately 4 mg/l (bulk and nano ZnO) to approximately 100 mg/l (nano CuO) and approximately 4000 mg/l (bulk CuO). Thus, considering an excellent correlation between both formats, 96-well microplate Flash Assay can be successfully used for high throughput evaluation of harmful properties of chemicals (including organic and inorganic NPs) to bacteria. SN - 0887-2333 UR - https://www.unboundmedicine.com/medline/citation/18400463/High_throughput_kinetic_Vibrio_fischeri_bioluminescence_inhibition_assay_for_study_of_toxic_effects_of_nanoparticles_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0887-2333(08)00049-0 DB - PRIME DP - Unbound Medicine ER -