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Recycling of docosahexaenoic acid in rat retinas during n-3 fatty acid deficiency.
J Lipid Res. 1991 Dec; 32(12):2009-17.JL

Abstract

About 50% of the fatty acids in retinal rod outer segments is docosahexaenoic acid [22:6(n-3)], a member of the linolenic acid [18:3(n-3)] family of essential fatty acids. Dietary deprivation of n-3 fatty acids leads to only modest changes in 22:6(n-3) levels in the retina. We investigated the mechanism(s) by which the retina conserves 22:6(n-3) during n-3 fatty acid deficiency. Weanling rats were fed diets containing 10% (wt/wt) hydrogenated coconut oil (no n-3 or n-6 fatty acids), linseed oil (high n-3, low n-6), or safflower oil (high n-6, less than 0.1% n-3) for 15 weeks. The turnover of phospholipid molecular species and the turnover and recycling of 22:6(n-3) in phospholipids of the rod outer segment membranes were examined after the intravitreal injection of [2-3H]glycerol and [4,5-3H]22:6(n-3), respectively. Animals were killed on selected days, and rod outer segment membranes, liver, and plasma were taken for lipid analyses. The half-lives (days) of individual phospholipid molecular species and total phospholipid 22:6(n-3) were calculated from the slopes of the regression lines of log specific activity versus time. There were no differences in the turnover rates of phospholipid molecular species among the three dietary groups, as determined by the disappearance of labeled glycerol. Thus, 22:6(n-3) is not conserved through a reduction in phospholipid turnover in rod outer segments. However, the half-life of [4,5-3H]22:6(n-3) in the linseed oil group (19 days) was significantly less than in the coconut oil (54 days) and safflower oil (not measurable) groups.(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Cullen Eye Institute, Houston, TX.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

1840067

Citation

Stinson, A M., et al. "Recycling of Docosahexaenoic Acid in Rat Retinas During N-3 Fatty Acid Deficiency." Journal of Lipid Research, vol. 32, no. 12, 1991, pp. 2009-17.
Stinson AM, Wiegand RD, Anderson RE. Recycling of docosahexaenoic acid in rat retinas during n-3 fatty acid deficiency. J Lipid Res. 1991;32(12):2009-17.
Stinson, A. M., Wiegand, R. D., & Anderson, R. E. (1991). Recycling of docosahexaenoic acid in rat retinas during n-3 fatty acid deficiency. Journal of Lipid Research, 32(12), 2009-17.
Stinson AM, Wiegand RD, Anderson RE. Recycling of Docosahexaenoic Acid in Rat Retinas During N-3 Fatty Acid Deficiency. J Lipid Res. 1991;32(12):2009-17. PubMed PMID: 1840067.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Recycling of docosahexaenoic acid in rat retinas during n-3 fatty acid deficiency. AU - Stinson,A M, AU - Wiegand,R D, AU - Anderson,R E, PY - 1991/12/1/pubmed PY - 1991/12/1/medline PY - 1991/12/1/entrez SP - 2009 EP - 17 JF - Journal of lipid research JO - J Lipid Res VL - 32 IS - 12 N2 - About 50% of the fatty acids in retinal rod outer segments is docosahexaenoic acid [22:6(n-3)], a member of the linolenic acid [18:3(n-3)] family of essential fatty acids. Dietary deprivation of n-3 fatty acids leads to only modest changes in 22:6(n-3) levels in the retina. We investigated the mechanism(s) by which the retina conserves 22:6(n-3) during n-3 fatty acid deficiency. Weanling rats were fed diets containing 10% (wt/wt) hydrogenated coconut oil (no n-3 or n-6 fatty acids), linseed oil (high n-3, low n-6), or safflower oil (high n-6, less than 0.1% n-3) for 15 weeks. The turnover of phospholipid molecular species and the turnover and recycling of 22:6(n-3) in phospholipids of the rod outer segment membranes were examined after the intravitreal injection of [2-3H]glycerol and [4,5-3H]22:6(n-3), respectively. Animals were killed on selected days, and rod outer segment membranes, liver, and plasma were taken for lipid analyses. The half-lives (days) of individual phospholipid molecular species and total phospholipid 22:6(n-3) were calculated from the slopes of the regression lines of log specific activity versus time. There were no differences in the turnover rates of phospholipid molecular species among the three dietary groups, as determined by the disappearance of labeled glycerol. Thus, 22:6(n-3) is not conserved through a reduction in phospholipid turnover in rod outer segments. However, the half-life of [4,5-3H]22:6(n-3) in the linseed oil group (19 days) was significantly less than in the coconut oil (54 days) and safflower oil (not measurable) groups.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0022-2275 UR - https://www.unboundmedicine.com/medline/citation/1840067/Recycling_of_docosahexaenoic_acid_in_rat_retinas_during_n_3_fatty_acid_deficiency_ L2 - http://www.jlr.org/cgi/pmidlookup?view=long&pmid=1840067 DB - PRIME DP - Unbound Medicine ER -