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Evaluation of three automated nucleic acid amplification systems for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in first-void urine specimens.
J Clin Microbiol. 2008 Jun; 46(6):2109-11.JC

Abstract

A total of 500 first-void urine specimens were tested for the presence of Chlamydia trachomatis and Neisseria gonorrhoeae nucleic acids using ProbeTec ET reagents on a Viper platform (BD Diagnostics, Mississauga, Ontario, Canada), Aptima Combo 2 reagents on a Tigris platform (Gen-Probe, Inc., San Diego, CA), and Abbott RealTime CT/NG reagents on an m2000 platform (Abbott Molecular Diagnostics, Des Plaines, IL). The performance of the three assays for detection of N. gonorrhoeae was comparable, but detection of C. trachomatis by the three assays showed more variation. All three platforms were suitable for the detection of C. trachomatis and N. gonorrhoeae, but additional factors, such as maximum daily specimen throughput, are important in evaluating automated systems for C. trachomatis and N. gonorrhoeae detection in high-volume laboratories.

Authors+Show Affiliations

Saskatchewan Disease Control Laboratory, 3211 Albert Street, Regina, Saskatchewan S4S 5W6, Canada. plevett@health.gov.sk.caNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

18400919

Citation

Levett, P N., et al. "Evaluation of Three Automated Nucleic Acid Amplification Systems for Detection of Chlamydia Trachomatis and Neisseria Gonorrhoeae in First-void Urine Specimens." Journal of Clinical Microbiology, vol. 46, no. 6, 2008, pp. 2109-11.
Levett PN, Brandt K, Olenius K, et al. Evaluation of three automated nucleic acid amplification systems for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in first-void urine specimens. J Clin Microbiol. 2008;46(6):2109-11.
Levett, P. N., Brandt, K., Olenius, K., Brown, C., Montgomery, K., & Horsman, G. B. (2008). Evaluation of three automated nucleic acid amplification systems for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in first-void urine specimens. Journal of Clinical Microbiology, 46(6), 2109-11. https://doi.org/10.1128/JCM.00043-08
Levett PN, et al. Evaluation of Three Automated Nucleic Acid Amplification Systems for Detection of Chlamydia Trachomatis and Neisseria Gonorrhoeae in First-void Urine Specimens. J Clin Microbiol. 2008;46(6):2109-11. PubMed PMID: 18400919.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of three automated nucleic acid amplification systems for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in first-void urine specimens. AU - Levett,P N, AU - Brandt,K, AU - Olenius,K, AU - Brown,C, AU - Montgomery,K, AU - Horsman,G B, Y1 - 2008/04/09/ PY - 2008/4/11/pubmed PY - 2008/7/24/medline PY - 2008/4/11/entrez SP - 2109 EP - 11 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 46 IS - 6 N2 - A total of 500 first-void urine specimens were tested for the presence of Chlamydia trachomatis and Neisseria gonorrhoeae nucleic acids using ProbeTec ET reagents on a Viper platform (BD Diagnostics, Mississauga, Ontario, Canada), Aptima Combo 2 reagents on a Tigris platform (Gen-Probe, Inc., San Diego, CA), and Abbott RealTime CT/NG reagents on an m2000 platform (Abbott Molecular Diagnostics, Des Plaines, IL). The performance of the three assays for detection of N. gonorrhoeae was comparable, but detection of C. trachomatis by the three assays showed more variation. All three platforms were suitable for the detection of C. trachomatis and N. gonorrhoeae, but additional factors, such as maximum daily specimen throughput, are important in evaluating automated systems for C. trachomatis and N. gonorrhoeae detection in high-volume laboratories. SN - 1098-660X UR - https://www.unboundmedicine.com/medline/citation/18400919/Evaluation_of_three_automated_nucleic_acid_amplification_systems_for_detection_of_Chlamydia_trachomatis_and_Neisseria_gonorrhoeae_in_first_void_urine_specimens_ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=18400919 DB - PRIME DP - Unbound Medicine ER -