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Construction and properties of a family of pACYC184-derived cloning vectors compatible with pBR322 and its derivatives.
Gene 1991; 102(1):75-8GENE

Abstract

A family of cloning vectors derived from plasmid pACYC184 and, therefore, compatible with pBR322 and its derivatives (especially the pUC family of vectors), is described. They all contain a multiple cloning site (MCS) and the lacZ alpha reporter gene for easy cloning. They have been grouped in three sets: (i) six of the vectors contain a chloramphenicol-resistance (CmR)-encoding gene and each a different MCS with 16 unique restriction sites overall; (ii) another six vectors contain a kanamycin-resistance (KmR)-encoding gene and the same six MCS; and (iii) two CmR vectors that contain the SP6 and T7 promoters flanking the MCS and lacZ alpha reporter gene of pUC18/19.

Authors+Show Affiliations

Departamento de Biología Molecular, Universidad de Cantabria, Santander, Spain.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1840539

Citation

Bartolomé, B, et al. "Construction and Properties of a Family of pACYC184-derived Cloning Vectors Compatible With pBR322 and Its Derivatives." Gene, vol. 102, no. 1, 1991, pp. 75-8.
Bartolomé B, Jubete Y, Martínez E, et al. Construction and properties of a family of pACYC184-derived cloning vectors compatible with pBR322 and its derivatives. Gene. 1991;102(1):75-8.
Bartolomé, B., Jubete, Y., Martínez, E., & de la Cruz, F. (1991). Construction and properties of a family of pACYC184-derived cloning vectors compatible with pBR322 and its derivatives. Gene, 102(1), pp. 75-8.
Bartolomé B, et al. Construction and Properties of a Family of pACYC184-derived Cloning Vectors Compatible With pBR322 and Its Derivatives. Gene. 1991 Jun 15;102(1):75-8. PubMed PMID: 1840539.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Construction and properties of a family of pACYC184-derived cloning vectors compatible with pBR322 and its derivatives. AU - Bartolomé,B, AU - Jubete,Y, AU - Martínez,E, AU - de la Cruz,F, PY - 1991/6/15/pubmed PY - 1991/6/15/medline PY - 1991/6/15/entrez SP - 75 EP - 8 JF - Gene JO - Gene VL - 102 IS - 1 N2 - A family of cloning vectors derived from plasmid pACYC184 and, therefore, compatible with pBR322 and its derivatives (especially the pUC family of vectors), is described. They all contain a multiple cloning site (MCS) and the lacZ alpha reporter gene for easy cloning. They have been grouped in three sets: (i) six of the vectors contain a chloramphenicol-resistance (CmR)-encoding gene and each a different MCS with 16 unique restriction sites overall; (ii) another six vectors contain a kanamycin-resistance (KmR)-encoding gene and the same six MCS; and (iii) two CmR vectors that contain the SP6 and T7 promoters flanking the MCS and lacZ alpha reporter gene of pUC18/19. SN - 0378-1119 UR - https://www.unboundmedicine.com/medline/citation/1840539/Construction_and_properties_of_a_family_of_pACYC184_derived_cloning_vectors_compatible_with_pBR322_and_its_derivatives_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0378-1119(91)90541-I DB - PRIME DP - Unbound Medicine ER -