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Neuropeptide FF receptors antagonist, RF9, attenuates opioid-evoked hypothermia in mice.
Peptides. 2008 Jul; 29(7):1183-90.P

Abstract

The present study used the endpoint of hypothermia to investigate opioid and neuropeptide FF (NPFF) interactions in conscious animals. Both opioid and NPFF systems played important roles in thermoregulation, which suggested a link between opioid receptors and NPFF receptors in the production of hypothermia. Therefore, we designed a study to investigate the relationship between opioid and NPFF in control of thermoregulation in mice. The selective NPFF receptors antagonist RF9 (30nmol) injected into the third ventricle failed to induce significant effect, but it completely antagonized the hypothermia of NPFF (45 nmol) after cerebral administration in mice. In addition, RF9 (30 nmol) co-injected i.c.v. in the third ventricle reduced the hypothermia induced by morphine (5nmol,) or nociceptin/orphanin FQ (N/OFQ) (2 nmol). Neither the classical opioid receptors antagonist naloxone (10 nmol) nor NOP receptor antagonist [Nphe(1)]NC(1-13)NH(2) (7.5 nmol) reduced the hypothermia induced by the central injection of NPFF at dose of 45 nmol. Co-injected with a low dose of NPFF (5 nmol), the hypothermia of morphine (5 nmol) or N/OFQ (2 nmol) was not modified. These results suggest that NPFF receptors activation is required for opioid to produce hypothermia. In contrast, NPFF-induced hypothermia is mainly mediated by its own receptors, independent of opioid receptors in the mouse brain. This interaction, quantitated in the present study, is the first evidence that NPFF receptors mediate opioid-induced hypothermia in conscious animals.

Authors+Show Affiliations

Key Laboratory of Preclinical Study for New Drugs of Gansu Province, Lanzhou University, 222 Tian Shui South Road, Lanzhou 730000, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18406009

Citation

Wang, Yi-Qing, et al. "Neuropeptide FF Receptors Antagonist, RF9, Attenuates Opioid-evoked Hypothermia in Mice." Peptides, vol. 29, no. 7, 2008, pp. 1183-90.
Wang YQ, Guo J, Wang SB, et al. Neuropeptide FF receptors antagonist, RF9, attenuates opioid-evoked hypothermia in mice. Peptides. 2008;29(7):1183-90.
Wang, Y. Q., Guo, J., Wang, S. B., Fang, Q., He, F., & Wang, R. (2008). Neuropeptide FF receptors antagonist, RF9, attenuates opioid-evoked hypothermia in mice. Peptides, 29(7), 1183-90. https://doi.org/10.1016/j.peptides.2008.02.016
Wang YQ, et al. Neuropeptide FF Receptors Antagonist, RF9, Attenuates Opioid-evoked Hypothermia in Mice. Peptides. 2008;29(7):1183-90. PubMed PMID: 18406009.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuropeptide FF receptors antagonist, RF9, attenuates opioid-evoked hypothermia in mice. AU - Wang,Yi-Qing, AU - Guo,Jia, AU - Wang,Sheng-Bin, AU - Fang,Quan, AU - He,Feng, AU - Wang,Rui, Y1 - 2008/03/04/ PY - 2008/01/23/received PY - 2008/02/25/revised PY - 2008/02/26/accepted PY - 2008/4/15/pubmed PY - 2008/9/23/medline PY - 2008/4/15/entrez SP - 1183 EP - 90 JF - Peptides JO - Peptides VL - 29 IS - 7 N2 - The present study used the endpoint of hypothermia to investigate opioid and neuropeptide FF (NPFF) interactions in conscious animals. Both opioid and NPFF systems played important roles in thermoregulation, which suggested a link between opioid receptors and NPFF receptors in the production of hypothermia. Therefore, we designed a study to investigate the relationship between opioid and NPFF in control of thermoregulation in mice. The selective NPFF receptors antagonist RF9 (30nmol) injected into the third ventricle failed to induce significant effect, but it completely antagonized the hypothermia of NPFF (45 nmol) after cerebral administration in mice. In addition, RF9 (30 nmol) co-injected i.c.v. in the third ventricle reduced the hypothermia induced by morphine (5nmol,) or nociceptin/orphanin FQ (N/OFQ) (2 nmol). Neither the classical opioid receptors antagonist naloxone (10 nmol) nor NOP receptor antagonist [Nphe(1)]NC(1-13)NH(2) (7.5 nmol) reduced the hypothermia induced by the central injection of NPFF at dose of 45 nmol. Co-injected with a low dose of NPFF (5 nmol), the hypothermia of morphine (5 nmol) or N/OFQ (2 nmol) was not modified. These results suggest that NPFF receptors activation is required for opioid to produce hypothermia. In contrast, NPFF-induced hypothermia is mainly mediated by its own receptors, independent of opioid receptors in the mouse brain. This interaction, quantitated in the present study, is the first evidence that NPFF receptors mediate opioid-induced hypothermia in conscious animals. SN - 0196-9781 UR - https://www.unboundmedicine.com/medline/citation/18406009/Neuropeptide_FF_receptors_antagonist_RF9_attenuates_opioid_evoked_hypothermia_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0196-9781(08)00110-1 DB - PRIME DP - Unbound Medicine ER -