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Validation of chiral capillary electrophoresis-electrospray ionization-mass spectrometry methods for ecstasy and methadone in plasma.
Electrophoresis. 2008 May; 29(10):2193-202.E

Abstract

Due to its selectivity and sensitivity, CE coupled to MS (CE-MS) has evolved as a useful analytical tool for determining drugs and metabolites in biological samples. A generic CE-ESI/MS method was developed for the enantioselective determination of basic compounds in plasma. The use of protein precipitation (PP) prior to a hydrodynamic injection (HD) was well adapted to high-concentration samples (>1 ppm) and allowed high throughput. In contrast, the combination of liquid-liquid extraction (LLE) and electrokinetic injection (EK) was time-consuming but did allow detection at the ppb level. Both approaches were fully validated according to ICH guidelines and SFSTP protocols for two pharmaceutical compounds (ecstasy and methadone (MTD)). Deuterated internal standards (IS) in the analytical procedures were used and good quantitative performance was obtained in terms of trueness and precision (repeatability and intermediate precision) since accuracy profiles were within the acceptance limits (30% for biological assay). Methods were linear over the concentration range of 0.50-175 ng/mL and 0.25-5 microg/mL for LLE-EK and PP-HD procedures, respectively. The LLE-EK methodology was finally successfully applied to quantitation of ecstasy and MTD in real cases obtained from toxicology.

Authors+Show Affiliations

Laboratory of Pharmaceutical Analytical Chemistry, School of Pharmaceutical Sciences - EPGL, University of Geneva, Geneva, Switzerland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

18409162

Citation

Schappler, Julie, et al. "Validation of Chiral Capillary Electrophoresis-electrospray Ionization-mass Spectrometry Methods for Ecstasy and Methadone in Plasma." Electrophoresis, vol. 29, no. 10, 2008, pp. 2193-202.
Schappler J, Guillarme D, Prat J, et al. Validation of chiral capillary electrophoresis-electrospray ionization-mass spectrometry methods for ecstasy and methadone in plasma. Electrophoresis. 2008;29(10):2193-202.
Schappler, J., Guillarme, D., Prat, J., Veuthey, J. L., & Rudaz, S. (2008). Validation of chiral capillary electrophoresis-electrospray ionization-mass spectrometry methods for ecstasy and methadone in plasma. Electrophoresis, 29(10), 2193-202. https://doi.org/10.1002/elps.200700464
Schappler J, et al. Validation of Chiral Capillary Electrophoresis-electrospray Ionization-mass Spectrometry Methods for Ecstasy and Methadone in Plasma. Electrophoresis. 2008;29(10):2193-202. PubMed PMID: 18409162.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Validation of chiral capillary electrophoresis-electrospray ionization-mass spectrometry methods for ecstasy and methadone in plasma. AU - Schappler,Julie, AU - Guillarme,Davy, AU - Prat,Josiane, AU - Veuthey,Jean-Luc, AU - Rudaz,Serge, PY - 2008/4/15/pubmed PY - 2008/9/4/medline PY - 2008/4/15/entrez SP - 2193 EP - 202 JF - Electrophoresis JO - Electrophoresis VL - 29 IS - 10 N2 - Due to its selectivity and sensitivity, CE coupled to MS (CE-MS) has evolved as a useful analytical tool for determining drugs and metabolites in biological samples. A generic CE-ESI/MS method was developed for the enantioselective determination of basic compounds in plasma. The use of protein precipitation (PP) prior to a hydrodynamic injection (HD) was well adapted to high-concentration samples (>1 ppm) and allowed high throughput. In contrast, the combination of liquid-liquid extraction (LLE) and electrokinetic injection (EK) was time-consuming but did allow detection at the ppb level. Both approaches were fully validated according to ICH guidelines and SFSTP protocols for two pharmaceutical compounds (ecstasy and methadone (MTD)). Deuterated internal standards (IS) in the analytical procedures were used and good quantitative performance was obtained in terms of trueness and precision (repeatability and intermediate precision) since accuracy profiles were within the acceptance limits (30% for biological assay). Methods were linear over the concentration range of 0.50-175 ng/mL and 0.25-5 microg/mL for LLE-EK and PP-HD procedures, respectively. The LLE-EK methodology was finally successfully applied to quantitation of ecstasy and MTD in real cases obtained from toxicology. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/18409162/Validation_of_chiral_capillary_electrophoresis_electrospray_ionization_mass_spectrometry_methods_for_ecstasy_and_methadone_in_plasma_ L2 - https://doi.org/10.1002/elps.200700464 DB - PRIME DP - Unbound Medicine ER -