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An organotypic model of skin to study photodamage and photoprotection in vitro.
J Am Acad Dermatol. 2008 May; 58(5 Suppl 2):S155-9.JA

Abstract

Acute or repetitive sun exposures are known to elicit cutaneous damages such as sunburn but also long-term effects such as photoaging or cancers. Determination of early biological events occurring after ultraviolet (UV) exposure is essential for photoprotection. Using skin reconstructed in vitro containing both a dermal equivalent and a fully differentiated epidermis, the effects of UV light (UVB and UVA) were investigated. UVB-induced damage was essentially epidermal, with the typical sunburn cells and DNA lesions, whereas UVA radiation-induced damage was mostly located within the dermal compartment. The model and end points used for UVB- and UVA-induced damages appeared to be very useful for the in vitro evaluation of sunscreens after topical application, in particular to investigate its protective effects against the effects of UVR, and allowed us to distinguish the efficiency of absorbers depending on their absorption spectrum.

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Authors+Show Affiliations

Bernerd F
L'Oréal Recherche, Clichy, France. fbernerd@recherche.loreal.com
Asselineau D
No affiliation info available

MeSH

Cells, CulturedHumansIn Vitro TechniquesKeratinocytesSkinSkin AgingSkin, ArtificialSunscreening AgentsUltraviolet Rays

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

18410802

Citation

Bernerd, Françoise, and Daniel Asselineau. "An Organotypic Model of Skin to Study Photodamage and Photoprotection in Vitro." Journal of the American Academy of Dermatology, vol. 58, no. 5 Suppl 2, 2008, pp. S155-9.
Bernerd F, Asselineau D. An organotypic model of skin to study photodamage and photoprotection in vitro. J Am Acad Dermatol. 2008;58(5 Suppl 2):S155-9.
Bernerd, F., & Asselineau, D. (2008). An organotypic model of skin to study photodamage and photoprotection in vitro. Journal of the American Academy of Dermatology, 58(5 Suppl 2), S155-9. https://doi.org/10.1016/j.jaad.2007.08.050
Bernerd F, Asselineau D. An Organotypic Model of Skin to Study Photodamage and Photoprotection in Vitro. J Am Acad Dermatol. 2008;58(5 Suppl 2):S155-9. PubMed PMID: 18410802.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An organotypic model of skin to study photodamage and photoprotection in vitro. AU - Bernerd,Françoise, AU - Asselineau,Daniel, PY - 2007/01/15/received PY - 2007/07/25/revised PY - 2007/08/01/accepted PY - 2008/4/25/pubmed PY - 2008/5/16/medline PY - 2008/4/25/entrez SP - S155 EP - 9 JF - Journal of the American Academy of Dermatology JO - J Am Acad Dermatol VL - 58 IS - 5 Suppl 2 N2 - Acute or repetitive sun exposures are known to elicit cutaneous damages such as sunburn but also long-term effects such as photoaging or cancers. Determination of early biological events occurring after ultraviolet (UV) exposure is essential for photoprotection. Using skin reconstructed in vitro containing both a dermal equivalent and a fully differentiated epidermis, the effects of UV light (UVB and UVA) were investigated. UVB-induced damage was essentially epidermal, with the typical sunburn cells and DNA lesions, whereas UVA radiation-induced damage was mostly located within the dermal compartment. The model and end points used for UVB- and UVA-induced damages appeared to be very useful for the in vitro evaluation of sunscreens after topical application, in particular to investigate its protective effects against the effects of UVR, and allowed us to distinguish the efficiency of absorbers depending on their absorption spectrum. SN - 1097-6787 UR - https://www.unboundmedicine.com/medline/citation/18410802/An_organotypic_model_of_skin_to_study_photodamage_and_photoprotection_in_vitro_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0190-9622(07)02414-0 DB - PRIME DP - Unbound Medicine ER -