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Bioprocess of uniform-sized crosslinked chitosan microspheres in rats following oral administration.
Eur J Pharm Biopharm. 2008 Aug; 69(3):878-86.EJ

Abstract

Chitosan microspheres have a great potential in pharmaceutical application. In this study, uniform-sized chitosan microspheres crosslinked with glutaraldehyde (CG microspheres) were prepared by Shirasu Porous Glass (SPG) membrane emulsification technique. Based on the characterizations of uniform size and autofluorescence, it was possible to develop a new detecting system for observing and quantifying the CG microspheres in rats with three different diameters (2.1, 7.2 and 12.5 microm) synchronously after oral administration. This system was a combination of scanning electron microscopy (SEM), laser scanning confocal microscope (LSCM) and flow cytometer technique, which showed the advantages of being simple, intuitionistic, repeatable and sensitive. After oral administration of three kinds of particles with different diameters, bioadhesion in gastrointestinal tract, absorption in gastrointestinal tract, distribution in systemic tissues, and biodegradation in reticuloendothelial system (RES) were studied firstly in detail. The CG microspheres showed different fates in bioadhesion, absorption and distribution according to their diameters, while the biodegradation also varied due to the different locations in RES. These original results would indicate a better way for the CG microspheres in the clinical application.

Authors+Show Affiliations

Chinese Academy of Sciences, Beijing, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18417329

Citation

Wei, Wei, et al. "Bioprocess of Uniform-sized Crosslinked Chitosan Microspheres in Rats Following Oral Administration." European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Fur Pharmazeutische Verfahrenstechnik E.V, vol. 69, no. 3, 2008, pp. 878-86.
Wei W, Wang LY, Yuan L, et al. Bioprocess of uniform-sized crosslinked chitosan microspheres in rats following oral administration. Eur J Pharm Biopharm. 2008;69(3):878-86.
Wei, W., Wang, L. Y., Yuan, L., Yang, X. D., Su, Z. G., & Ma, G. H. (2008). Bioprocess of uniform-sized crosslinked chitosan microspheres in rats following oral administration. European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Fur Pharmazeutische Verfahrenstechnik E.V, 69(3), 878-86. https://doi.org/10.1016/j.ejpb.2008.02.011
Wei W, et al. Bioprocess of Uniform-sized Crosslinked Chitosan Microspheres in Rats Following Oral Administration. Eur J Pharm Biopharm. 2008;69(3):878-86. PubMed PMID: 18417329.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bioprocess of uniform-sized crosslinked chitosan microspheres in rats following oral administration. AU - Wei,Wei, AU - Wang,Lian-Yan, AU - Yuan,Lan, AU - Yang,Xiao-Da, AU - Su,Zhi-Guo, AU - Ma,Guang-Hui, Y1 - 2008/02/19/ PY - 2007/09/22/received PY - 2007/12/07/revised PY - 2008/02/01/accepted PY - 2008/4/18/pubmed PY - 2008/10/31/medline PY - 2008/4/18/entrez SP - 878 EP - 86 JF - European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V JO - Eur J Pharm Biopharm VL - 69 IS - 3 N2 - Chitosan microspheres have a great potential in pharmaceutical application. In this study, uniform-sized chitosan microspheres crosslinked with glutaraldehyde (CG microspheres) were prepared by Shirasu Porous Glass (SPG) membrane emulsification technique. Based on the characterizations of uniform size and autofluorescence, it was possible to develop a new detecting system for observing and quantifying the CG microspheres in rats with three different diameters (2.1, 7.2 and 12.5 microm) synchronously after oral administration. This system was a combination of scanning electron microscopy (SEM), laser scanning confocal microscope (LSCM) and flow cytometer technique, which showed the advantages of being simple, intuitionistic, repeatable and sensitive. After oral administration of three kinds of particles with different diameters, bioadhesion in gastrointestinal tract, absorption in gastrointestinal tract, distribution in systemic tissues, and biodegradation in reticuloendothelial system (RES) were studied firstly in detail. The CG microspheres showed different fates in bioadhesion, absorption and distribution according to their diameters, while the biodegradation also varied due to the different locations in RES. These original results would indicate a better way for the CG microspheres in the clinical application. SN - 0939-6411 UR - https://www.unboundmedicine.com/medline/citation/18417329/Bioprocess_of_uniform_sized_crosslinked_chitosan_microspheres_in_rats_following_oral_administration_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0939-6411(08)00041-6 DB - PRIME DP - Unbound Medicine ER -