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LC-MS analysis of trimethoxyamphetamine designer drugs (TMA series) from urine samples.
J Chromatogr B Analyt Technol Biomed Life Sci. 2008 May 01; 867(1):126-30.JC

Abstract

A sensitive liquid chromatography-mass spectrometric (LC-MS) method for quantification of an active psychedelic hallucinogenic drugs (trimethoxyamphetamines) in human urine after solid-phase extraction (SPE) with C(18) cartridge was developed and validated. Chromatographic separation was achieved on reversed-phase Phenomenex 3.0 microm Polar Plus column (150 mm x 2.1 mm) with acetonitrile -0.2% acetic acid as mobile-phase and the step gradient elution resulted in a total run time of about 20 min. The analytes were detected by using an electrospray positive ionization mass spectrometry in selected ion monitoring (SIM) mode. In the evaluated concentration range (10-200 ng/mL) (R(2) > or = 0.998) a good linear relationship was obtained. The lower limits of detection (LLODs) and quantification (LLOQs) ranged from 4.26 to 9.12 ng/mL and from 13.18 to 29.22 ng/mL, respectively. Average recoveries ranged from 68.52 to 97.90% in urine at the concentrations of 25, 50 and 100 ng/mL. Intra- and inter-day relative standard deviations were 3.70-10.77% and 7.63-12.94%, respectively. This LC-MS method proved to be robust and reliable, and suitable for the use as a confirmation method in clinical urine drug testing.

Authors+Show Affiliations

Dipartimento Farmaco Chimico Tossicologico, Università degli Studi di Sassari, via Muroni 23/a, 07100 Sassari, Italy. marvi@uniss.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18417429

Citation

Nieddu, Maria, et al. "LC-MS Analysis of Trimethoxyamphetamine Designer Drugs (TMA Series) From Urine Samples." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 867, no. 1, 2008, pp. 126-30.
Nieddu M, Boatto G, Pirisi MA, et al. LC-MS analysis of trimethoxyamphetamine designer drugs (TMA series) from urine samples. J Chromatogr B Analyt Technol Biomed Life Sci. 2008;867(1):126-30.
Nieddu, M., Boatto, G., Pirisi, M. A., Azara, E., & Marchetti, M. (2008). LC-MS analysis of trimethoxyamphetamine designer drugs (TMA series) from urine samples. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 867(1), 126-30. https://doi.org/10.1016/j.jchromb.2008.03.027
Nieddu M, et al. LC-MS Analysis of Trimethoxyamphetamine Designer Drugs (TMA Series) From Urine Samples. J Chromatogr B Analyt Technol Biomed Life Sci. 2008 May 1;867(1):126-30. PubMed PMID: 18417429.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - LC-MS analysis of trimethoxyamphetamine designer drugs (TMA series) from urine samples. AU - Nieddu,Maria, AU - Boatto,Gianpiero, AU - Pirisi,Maria Antonietta, AU - Azara,Emanuela, AU - Marchetti,Mauro, Y1 - 2008/04/04/ PY - 2008/01/23/received PY - 2008/03/20/revised PY - 2008/03/30/accepted PY - 2008/4/18/pubmed PY - 2008/8/6/medline PY - 2008/4/18/entrez SP - 126 EP - 30 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 867 IS - 1 N2 - A sensitive liquid chromatography-mass spectrometric (LC-MS) method for quantification of an active psychedelic hallucinogenic drugs (trimethoxyamphetamines) in human urine after solid-phase extraction (SPE) with C(18) cartridge was developed and validated. Chromatographic separation was achieved on reversed-phase Phenomenex 3.0 microm Polar Plus column (150 mm x 2.1 mm) with acetonitrile -0.2% acetic acid as mobile-phase and the step gradient elution resulted in a total run time of about 20 min. The analytes were detected by using an electrospray positive ionization mass spectrometry in selected ion monitoring (SIM) mode. In the evaluated concentration range (10-200 ng/mL) (R(2) > or = 0.998) a good linear relationship was obtained. The lower limits of detection (LLODs) and quantification (LLOQs) ranged from 4.26 to 9.12 ng/mL and from 13.18 to 29.22 ng/mL, respectively. Average recoveries ranged from 68.52 to 97.90% in urine at the concentrations of 25, 50 and 100 ng/mL. Intra- and inter-day relative standard deviations were 3.70-10.77% and 7.63-12.94%, respectively. This LC-MS method proved to be robust and reliable, and suitable for the use as a confirmation method in clinical urine drug testing. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/18417429/LC_MS_analysis_of_trimethoxyamphetamine_designer_drugs__TMA_series__from_urine_samples_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(08)00200-6 DB - PRIME DP - Unbound Medicine ER -