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Genotoxic effects of p,p'-DDT (1,1,1-trichloro-2,2-bis-(chlorophenyl)ethane) and its metabolites in Zebra mussel (D. polymorpha) by SCGE assay and micronucleus test.
Environ Mol Mutagen. 2008 Jun; 49(5):406-15.EM

Abstract

This is the first study to evaluate the potential genotoxicity of p,p'-DDT (1,1,1-trichloro-2,2-bis-(chlorophenyl)ethane) and its metabolites (p,p'-DDD and p,p'-DDE) in the sentinel mollusc Zebra mussel (Dreissena polymorpha). DNA damage was measured using the single cell gel electrophoresis (SCGE) assay and the micronucleus test (MN test), which represent two of the more sensitive biomarkers for genotoxicity evaluation. Three different concentrations (0.1, 2, and 10 mug/L) of each compound were administered in water for 168 hr, maintaining mussels at constant laboratory conditions and collecting several specimens every 48 hr for biochemical analyses. At the same time, the bioaccumulation process and the concentration/effect relationship were checked by GC-MS/MS analyses of mussel soft tissues. The SCGE assay results showed a clear and significant (P < 0.05) relationship between DNA injuries and tested doses for all the homologues throughout the 7-day exposure period. The final DNA damage due to p,p'-DDE was almost double that of the other two homologues that showed the same toxicity pattern. The micronucleus frequency analysis confirmed the genotoxicity potential of the three homologues and p,p'-DDE showed the highest irreversible DNA damage. The capability of Zebra mussels to biotransform the administered compound in the other homologues was demonstrated by multiple regression analyses carried out between the MN frequencies and the concentrations of the different homologues in the mussel soft tissues. A greater genotoxic potential of the p,p'-DDE with respect to the other two chemicals was revealed.

Authors+Show Affiliations

Department of Biology, University of Milan, via Celoria 26, Milan, Italy. andrea.binelli@unimi.itNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18418866

Citation

Binelli, Andrea, et al. "Genotoxic Effects of p,p'-DDT (1,1,1-trichloro-2,2-bis-(chlorophenyl)ethane) and Its Metabolites in Zebra Mussel (D. Polymorpha) By SCGE Assay and Micronucleus Test." Environmental and Molecular Mutagenesis, vol. 49, no. 5, 2008, pp. 406-15.
Binelli A, Riva C, Cogni D, et al. Genotoxic effects of p,p'-DDT (1,1,1-trichloro-2,2-bis-(chlorophenyl)ethane) and its metabolites in Zebra mussel (D. polymorpha) by SCGE assay and micronucleus test. Environ Mol Mutagen. 2008;49(5):406-15.
Binelli, A., Riva, C., Cogni, D., & Provini, A. (2008). Genotoxic effects of p,p'-DDT (1,1,1-trichloro-2,2-bis-(chlorophenyl)ethane) and its metabolites in Zebra mussel (D. polymorpha) by SCGE assay and micronucleus test. Environmental and Molecular Mutagenesis, 49(5), 406-15. https://doi.org/10.1002/em.20400
Binelli A, et al. Genotoxic Effects of p,p'-DDT (1,1,1-trichloro-2,2-bis-(chlorophenyl)ethane) and Its Metabolites in Zebra Mussel (D. Polymorpha) By SCGE Assay and Micronucleus Test. Environ Mol Mutagen. 2008;49(5):406-15. PubMed PMID: 18418866.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genotoxic effects of p,p'-DDT (1,1,1-trichloro-2,2-bis-(chlorophenyl)ethane) and its metabolites in Zebra mussel (D. polymorpha) by SCGE assay and micronucleus test. AU - Binelli,Andrea, AU - Riva,Consuelo, AU - Cogni,Daniele, AU - Provini,Alfredo, PY - 2008/4/18/pubmed PY - 2008/6/28/medline PY - 2008/4/18/entrez SP - 406 EP - 15 JF - Environmental and molecular mutagenesis JO - Environ Mol Mutagen VL - 49 IS - 5 N2 - This is the first study to evaluate the potential genotoxicity of p,p'-DDT (1,1,1-trichloro-2,2-bis-(chlorophenyl)ethane) and its metabolites (p,p'-DDD and p,p'-DDE) in the sentinel mollusc Zebra mussel (Dreissena polymorpha). DNA damage was measured using the single cell gel electrophoresis (SCGE) assay and the micronucleus test (MN test), which represent two of the more sensitive biomarkers for genotoxicity evaluation. Three different concentrations (0.1, 2, and 10 mug/L) of each compound were administered in water for 168 hr, maintaining mussels at constant laboratory conditions and collecting several specimens every 48 hr for biochemical analyses. At the same time, the bioaccumulation process and the concentration/effect relationship were checked by GC-MS/MS analyses of mussel soft tissues. The SCGE assay results showed a clear and significant (P < 0.05) relationship between DNA injuries and tested doses for all the homologues throughout the 7-day exposure period. The final DNA damage due to p,p'-DDE was almost double that of the other two homologues that showed the same toxicity pattern. The micronucleus frequency analysis confirmed the genotoxicity potential of the three homologues and p,p'-DDE showed the highest irreversible DNA damage. The capability of Zebra mussels to biotransform the administered compound in the other homologues was demonstrated by multiple regression analyses carried out between the MN frequencies and the concentrations of the different homologues in the mussel soft tissues. A greater genotoxic potential of the p,p'-DDE with respect to the other two chemicals was revealed. SN - 1098-2280 UR - https://www.unboundmedicine.com/medline/citation/18418866/Genotoxic_effects_of_pp'_DDT__111_trichloro_22_bis__chlorophenyl_ethane__and_its_metabolites_in_Zebra_mussel__D__polymorpha__by_SCGE_assay_and_micronucleus_test_ L2 - https://doi.org/10.1002/em.20400 DB - PRIME DP - Unbound Medicine ER -