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Protective role of vitamin E on the microcystin-induced oxidative stress in tilapia fish (Oreochromis niloticus).
Environ Toxicol Chem. 2008 May; 27(5):1152-9.ET

Abstract

Microcystins (MCs) are potent hepatotoxins produced by cyanobacteria in water systems that induce oxidative stress in fish. The present study investigated the effect of vitamin E pretreatment on MC-induced oxidative damage in the liver, kidneys, and gills of tilapia fish (Oreochromis niloticus). Groups of fish were fed vitamin E supplements (200 or 700 mg per kilogram of diet) for 7 d or received only commercial fish food and then were exposed to a single oral dose of cyanobacterial cells (120 microg of MC-LR [2:Leu, 4:Arg] per fish), and were sacrificed in 24 h. The potential benefits of vitamin E were evaluated based on lipid peroxidation (LPO), protein oxidation, catalase (CAT, Enzyme Commission [EC] 1.11.1.6), superoxide dismutase (EC 1.15.1.1), glutathione peroxidase (EC 1.11.1.9), glutathione reductase (EC 1.8.1.7), and the ratio of reduced glutathione to oxidized glutathione (GSH/GSSG). Microcystins induced a 1.6-fold increase in LPO values in liver, whereas vitamin E-pretreated fish showed no alteration on this biomarker. Enzyme activities levels were also ameliorated by the chemoprotectant, whereas protein oxidation and GSH/GSSG did not show any significant change. The higher vitamin E dose used proved to have the greater protective effects, particularly on the biomarkers LPO and CAT. The results show that vitamin E could have a potential use as a preventive or therapeutic measure in MC-exposed fish.

Authors+Show Affiliations

Area of Toxicology, Faculty of Pharmacy, University of Seville, Seville, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18419199

Citation

Prieto, Ana I., et al. "Protective Role of Vitamin E On the Microcystin-induced Oxidative Stress in Tilapia Fish (Oreochromis Niloticus)." Environmental Toxicology and Chemistry, vol. 27, no. 5, 2008, pp. 1152-9.
Prieto AI, Jos A, Pichardo S, et al. Protective role of vitamin E on the microcystin-induced oxidative stress in tilapia fish (Oreochromis niloticus). Environ Toxicol Chem. 2008;27(5):1152-9.
Prieto, A. I., Jos, A., Pichardo, S., Moreno, I., & Cameán, A. M. (2008). Protective role of vitamin E on the microcystin-induced oxidative stress in tilapia fish (Oreochromis niloticus). Environmental Toxicology and Chemistry, 27(5), 1152-9. https://doi.org/10.1897/07-496.1
Prieto AI, et al. Protective Role of Vitamin E On the Microcystin-induced Oxidative Stress in Tilapia Fish (Oreochromis Niloticus). Environ Toxicol Chem. 2008;27(5):1152-9. PubMed PMID: 18419199.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective role of vitamin E on the microcystin-induced oxidative stress in tilapia fish (Oreochromis niloticus). AU - Prieto,Ana I, AU - Jos,Angeles, AU - Pichardo,Silvia, AU - Moreno,Isabel, AU - Cameán,Ana M, PY - 2007/11/15/received PY - 2007/11/21/accepted PY - 2008/4/19/pubmed PY - 2008/8/5/medline PY - 2008/4/19/entrez SP - 1152 EP - 9 JF - Environmental toxicology and chemistry JO - Environ Toxicol Chem VL - 27 IS - 5 N2 - Microcystins (MCs) are potent hepatotoxins produced by cyanobacteria in water systems that induce oxidative stress in fish. The present study investigated the effect of vitamin E pretreatment on MC-induced oxidative damage in the liver, kidneys, and gills of tilapia fish (Oreochromis niloticus). Groups of fish were fed vitamin E supplements (200 or 700 mg per kilogram of diet) for 7 d or received only commercial fish food and then were exposed to a single oral dose of cyanobacterial cells (120 microg of MC-LR [2:Leu, 4:Arg] per fish), and were sacrificed in 24 h. The potential benefits of vitamin E were evaluated based on lipid peroxidation (LPO), protein oxidation, catalase (CAT, Enzyme Commission [EC] 1.11.1.6), superoxide dismutase (EC 1.15.1.1), glutathione peroxidase (EC 1.11.1.9), glutathione reductase (EC 1.8.1.7), and the ratio of reduced glutathione to oxidized glutathione (GSH/GSSG). Microcystins induced a 1.6-fold increase in LPO values in liver, whereas vitamin E-pretreated fish showed no alteration on this biomarker. Enzyme activities levels were also ameliorated by the chemoprotectant, whereas protein oxidation and GSH/GSSG did not show any significant change. The higher vitamin E dose used proved to have the greater protective effects, particularly on the biomarkers LPO and CAT. The results show that vitamin E could have a potential use as a preventive or therapeutic measure in MC-exposed fish. SN - 0730-7268 UR - https://www.unboundmedicine.com/medline/citation/18419199/Protective_role_of_vitamin_E_on_the_microcystin_induced_oxidative_stress_in_tilapia_fish__Oreochromis_niloticus__ L2 - https://doi.org/10.1897/07-496.1 DB - PRIME DP - Unbound Medicine ER -