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[Protective effect of intralipid on myocardial ischemia/reperfusion injury in isolated rat heart].
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2008 Apr; 20(4):227-30.ZW

Abstract

OBJECTIVE

To investigate whether intralipid could protect perfused hearts of rats against ischemia/reperfusion (I/R) injury when it was administered before (preconditioning) or after the adverse ischemic event (postconditioning), in order to ascertain if intralipid would be a novel therapeutic strategy for myocardial I/R injury.

METHODS

Studies were conducted in Langendorff-perfused isolated rat hearts. Twenty-four male Sprague-Dawley (SD) rats were divided into 3 groups randomly. The experimental procedure consisted of balance perfusion for 50 minutes, warm global ischemia (37 centigrade) for 40 minutes and 120 minutes of reperfusion. Preconditioning of hearts in myocardial preconditioning (I-preC group) consisted of 15 minutes of intralipid followed by 15 minutes of wash out before ischemia for 40 minutes and reperfusion for 120 minutes. In myocardial postconditioning (I-postC group) rat hearts were perfused with intralipid for 15 minutes at the onset of reperfusion. Hearts without intralipid treatment served as ischemic control (ISCH) group. Left ventricular mechanical function [heart rate (HR), left ventricular end-diastolic pressure (LVEDP), left ventricular diastolic pressure (LVDP), maximal change rate of intraventricular pressure rise/down (+/-dp/dt max)] were measured during the experiment, cardiac enzyme activity [creatine kinase (CK), lactate dehydrogenase (LDH)] were determined at 20 minutes after balance and 60 minutes after reperfusion.

RESULTS

Comparing with ISCH group, the LVDP and +dp/dt max in the I-postC group were significantly higher and LVDEP, -dp/dt max were lower when compared with ISCH groups during reperfusion (all P<0.05). There were no significant differences in above indexes in I-preC group. As compared with the ISCH group, the content of LDH and CK in I-preC and I-postC were significantly lower at 60 minutes after reperfusion (all P<0.05). However, there were no significant differences between the I-preC and I-postC groups with respect to the levels of LDH and CK. The infarct size (IS) of I-preC and I-postC was markedly smaller than that of the ISCH group at 120 minutes of reperfusion [(21.6+/-1.8)%, (15.9+/-1.3)% vs. (46.5+/-3.9)%, both P<0.05]. The IS did not differ between the I-preC and I-postC groups (P>0.05).

CONCLUSION

Intralipid administered before or after ischemia can decrease the level of CK, LDH and IS during reperfusion in isolated rat hearts. Intralipid postconditioning improves mechanical function.

Authors+Show Affiliations

Liu SL
Laboratory of Anesthesia and Critical Care Medicine, West China Hospital, Sichuan University, Chendu 610041, Sichuan, China. lslsxmu@sina.com
Wang Y
No affiliation info available
Wang RR
No affiliation info available
Chai YF
No affiliation info available
Wu W
No affiliation info available
Huang H
No affiliation info available
Liu J
No affiliation info available

MeSH

AnimalsDisease Models, AnimalFat Emulsions, IntravenousIschemic Preconditioning, MyocardialMaleMyocardial Reperfusion InjuryMyocardiumRandom AllocationRatsRats, Sprague-Dawley

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

18419958

Citation

Liu, Shan-ling, et al. "[Protective Effect of Intralipid On Myocardial Ischemia/reperfusion Injury in Isolated Rat Heart]." Zhongguo Wei Zhong Bing Ji Jiu Yi Xue = Chinese Critical Care Medicine = Zhongguo Weizhongbing Jijiuyixue, vol. 20, no. 4, 2008, pp. 227-30.
Liu SL, Wang Y, Wang RR, et al. [Protective effect of intralipid on myocardial ischemia/reperfusion injury in isolated rat heart]. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2008;20(4):227-30.
Liu, S. L., Wang, Y., Wang, R. R., Chai, Y. F., Wu, W., Huang, H., & Liu, J. (2008). [Protective effect of intralipid on myocardial ischemia/reperfusion injury in isolated rat heart]. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue = Chinese Critical Care Medicine = Zhongguo Weizhongbing Jijiuyixue, 20(4), 227-30.
Liu SL, et al. [Protective Effect of Intralipid On Myocardial Ischemia/reperfusion Injury in Isolated Rat Heart]. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2008;20(4):227-30. PubMed PMID: 18419958.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Protective effect of intralipid on myocardial ischemia/reperfusion injury in isolated rat heart]. AU - Liu,Shan-ling, AU - Wang,Ying, AU - Wang,Ru-rong, AU - Chai,Yun-fei, AU - Wu,Wei, AU - Huang,Han, AU - Liu,Jin, PY - 2008/4/19/pubmed PY - 2010/1/6/medline PY - 2008/4/19/entrez SP - 227 EP - 30 JF - Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue JO - Zhongguo Wei Zhong Bing Ji Jiu Yi Xue VL - 20 IS - 4 N2 - OBJECTIVE: To investigate whether intralipid could protect perfused hearts of rats against ischemia/reperfusion (I/R) injury when it was administered before (preconditioning) or after the adverse ischemic event (postconditioning), in order to ascertain if intralipid would be a novel therapeutic strategy for myocardial I/R injury. METHODS: Studies were conducted in Langendorff-perfused isolated rat hearts. Twenty-four male Sprague-Dawley (SD) rats were divided into 3 groups randomly. The experimental procedure consisted of balance perfusion for 50 minutes, warm global ischemia (37 centigrade) for 40 minutes and 120 minutes of reperfusion. Preconditioning of hearts in myocardial preconditioning (I-preC group) consisted of 15 minutes of intralipid followed by 15 minutes of wash out before ischemia for 40 minutes and reperfusion for 120 minutes. In myocardial postconditioning (I-postC group) rat hearts were perfused with intralipid for 15 minutes at the onset of reperfusion. Hearts without intralipid treatment served as ischemic control (ISCH) group. Left ventricular mechanical function [heart rate (HR), left ventricular end-diastolic pressure (LVEDP), left ventricular diastolic pressure (LVDP), maximal change rate of intraventricular pressure rise/down (+/-dp/dt max)] were measured during the experiment, cardiac enzyme activity [creatine kinase (CK), lactate dehydrogenase (LDH)] were determined at 20 minutes after balance and 60 minutes after reperfusion. RESULTS: Comparing with ISCH group, the LVDP and +dp/dt max in the I-postC group were significantly higher and LVDEP, -dp/dt max were lower when compared with ISCH groups during reperfusion (all P<0.05). There were no significant differences in above indexes in I-preC group. As compared with the ISCH group, the content of LDH and CK in I-preC and I-postC were significantly lower at 60 minutes after reperfusion (all P<0.05). However, there were no significant differences between the I-preC and I-postC groups with respect to the levels of LDH and CK. The infarct size (IS) of I-preC and I-postC was markedly smaller than that of the ISCH group at 120 minutes of reperfusion [(21.6+/-1.8)%, (15.9+/-1.3)% vs. (46.5+/-3.9)%, both P<0.05]. The IS did not differ between the I-preC and I-postC groups (P>0.05). CONCLUSION: Intralipid administered before or after ischemia can decrease the level of CK, LDH and IS during reperfusion in isolated rat hearts. Intralipid postconditioning improves mechanical function. SN - 1003-0603 UR - https://www.unboundmedicine.com/medline/citation/18419958/[Protective_effect_of_intralipid_on_myocardial_ischemia/reperfusion_injury_in_isolated_rat_heart]_ DB - PRIME DP - Unbound Medicine ER -