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Pharmacological basis for use of Pistacia integerrima leaves in hyperuricemia and gout.
J Ethnopharmacol. 2008 May 22; 117(3):478-82.JE

Abstract

ETHNOPHARMACOLOGICAL SIGNIFICANCE

Pistacia integerrima Stew ex. Brandis is an important component of commonly dispensed traditional dosage forms. We wished to determine whether polyphenolic constituents of this plant could be useful in oxidative stress and have potential to counter hyperuricemia.

MATERIAL AND METHODS

Radical scavenging activity was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and xanthine oxidase (XO) inhibitory activity assay in vitro. Fructose (FRS) induced hyperuricemic animal model was used to asses the serum uric acid (UA) lowering effect by plant products.

RESULTS

Ethyl acetate and n-BuOH fractions had the highest DPPH radical scavenging activity. Fifty percent inhibitory concentration (IC(50)) was 6 and 7.6 microg/ml respectively. It was less than quercetin (IC(50) 0.95 microg/ml) and ascorbic acid (IC(50) 1.76 microg/ml). Xanthine oxidase inhibitory activity was comparable between n-BuOH and EtOAc (IC(50) 19 and 20 microg/ml) extracts but less than quercetin (IC(50) 0.65 microg/ml) and allopurinol (IC(50) 0.10 microg/ml). The antioxidant activity as well as the inhibitory activity towards the enzyme XO by quercetin-3-O-beta-d-glucopyranoside (5), kaempferol-3-O-beta-d-glucopyranoside (6), quercetin-3-O-(6''-O-syringyl)-beta-d-glucopyranoside (7), kaempferol-3-O-(4''-O-galloyl)-alpha-l-arabinopyranoside (8), rutin (4) together with aglycons, quercetin (1), kaempferol (2) and apigenin (3) was promising to continue in vivo hypouricemic studies. Ethyl acetate extract had dose dependent UA lowering effect in hyperuricemic mice. This effect was comparable with quercetin but less than allopurinol.

CONCLUSIONS

These findings are encouraging to plan clinical studies in hyperuricemic patients.

Authors+Show Affiliations

College of Medical Sciences, National University of Sciences and Technology, Rawalpindi 46000, Pakistan. naseemsaud@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18420362

Citation

Ahmad, Naseem Saud, et al. "Pharmacological Basis for Use of Pistacia Integerrima Leaves in Hyperuricemia and Gout." Journal of Ethnopharmacology, vol. 117, no. 3, 2008, pp. 478-82.
Ahmad NS, Farman M, Najmi MH, et al. Pharmacological basis for use of Pistacia integerrima leaves in hyperuricemia and gout. J Ethnopharmacol. 2008;117(3):478-82.
Ahmad, N. S., Farman, M., Najmi, M. H., Mian, K. B., & Hasan, A. (2008). Pharmacological basis for use of Pistacia integerrima leaves in hyperuricemia and gout. Journal of Ethnopharmacology, 117(3), 478-82. https://doi.org/10.1016/j.jep.2008.02.031
Ahmad NS, et al. Pharmacological Basis for Use of Pistacia Integerrima Leaves in Hyperuricemia and Gout. J Ethnopharmacol. 2008 May 22;117(3):478-82. PubMed PMID: 18420362.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pharmacological basis for use of Pistacia integerrima leaves in hyperuricemia and gout. AU - Ahmad,Naseem Saud, AU - Farman,Muhammad, AU - Najmi,Muzammil Hasan, AU - Mian,Kouser Bashir, AU - Hasan,Aurangzeb, Y1 - 2008/03/04/ PY - 2007/07/12/received PY - 2008/02/07/revised PY - 2008/02/25/accepted PY - 2008/4/19/pubmed PY - 2008/10/7/medline PY - 2008/4/19/entrez SP - 478 EP - 82 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 117 IS - 3 N2 - ETHNOPHARMACOLOGICAL SIGNIFICANCE: Pistacia integerrima Stew ex. Brandis is an important component of commonly dispensed traditional dosage forms. We wished to determine whether polyphenolic constituents of this plant could be useful in oxidative stress and have potential to counter hyperuricemia. MATERIAL AND METHODS: Radical scavenging activity was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and xanthine oxidase (XO) inhibitory activity assay in vitro. Fructose (FRS) induced hyperuricemic animal model was used to asses the serum uric acid (UA) lowering effect by plant products. RESULTS: Ethyl acetate and n-BuOH fractions had the highest DPPH radical scavenging activity. Fifty percent inhibitory concentration (IC(50)) was 6 and 7.6 microg/ml respectively. It was less than quercetin (IC(50) 0.95 microg/ml) and ascorbic acid (IC(50) 1.76 microg/ml). Xanthine oxidase inhibitory activity was comparable between n-BuOH and EtOAc (IC(50) 19 and 20 microg/ml) extracts but less than quercetin (IC(50) 0.65 microg/ml) and allopurinol (IC(50) 0.10 microg/ml). The antioxidant activity as well as the inhibitory activity towards the enzyme XO by quercetin-3-O-beta-d-glucopyranoside (5), kaempferol-3-O-beta-d-glucopyranoside (6), quercetin-3-O-(6''-O-syringyl)-beta-d-glucopyranoside (7), kaempferol-3-O-(4''-O-galloyl)-alpha-l-arabinopyranoside (8), rutin (4) together with aglycons, quercetin (1), kaempferol (2) and apigenin (3) was promising to continue in vivo hypouricemic studies. Ethyl acetate extract had dose dependent UA lowering effect in hyperuricemic mice. This effect was comparable with quercetin but less than allopurinol. CONCLUSIONS: These findings are encouraging to plan clinical studies in hyperuricemic patients. SN - 0378-8741 UR - https://www.unboundmedicine.com/medline/citation/18420362/Pharmacological_basis_for_use_of_Pistacia_integerrima_leaves_in_hyperuricemia_and_gout_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(08)00115-3 DB - PRIME DP - Unbound Medicine ER -