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A novel gene, ecl1(+), extends the chronological lifespan in fission yeast.
FEMS Yeast Res. 2008 Jun; 8(4):520-30.FY

Abstract

We have identified a novel gene from Schizosaccharomyces pombe that we have named ecl1(+) (extender of the chronological lifespan). When ecl1(+) is provided on a high-copy number plasmid, it extends the viability of both the Deltasty1 MAP kinase mutant and the wild-type cells after entry into the stationary phase. ecl1(+) encodes an 80-amino acid polypeptide that had not been annotated in the current database. The ecl1(+)-mRNA increases transiently when the growth phase is changed from the log phase to the stationary phase. The Ecl1 protein is localized in the nucleus. Calorie restriction extends the chronological lifespan of wild-type and Deltaecl1 cells but not ecl1(+)-overproducing cells. The Deltapka1 mutant shows little, if any, additional extension of viability when Ecl1 is overproduced. The ste11(+) gene that is negatively controlled by Pka1 is up regulated when Ecl1 is overproduced. From these results we propose that the effect of Ecl1 overproduction may be mainly linked to and negatively affects the Pka1-dependent pathway.

Authors+Show Affiliations

Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Chikusa-ku, Nagoya, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18422613

Citation

Ohtsuka, Hokuto, et al. "A Novel Gene, Ecl1(+), Extends the Chronological Lifespan in Fission Yeast." FEMS Yeast Research, vol. 8, no. 4, 2008, pp. 520-30.
Ohtsuka H, Mita S, Ogawa Y, et al. A novel gene, ecl1(+), extends the chronological lifespan in fission yeast. FEMS Yeast Res. 2008;8(4):520-30.
Ohtsuka, H., Mita, S., Ogawa, Y., Azuma, K., Ito, H., & Aiba, H. (2008). A novel gene, ecl1(+), extends the chronological lifespan in fission yeast. FEMS Yeast Research, 8(4), 520-30. https://doi.org/10.1111/j.1567-1364.2008.00379.x
Ohtsuka H, et al. A Novel Gene, Ecl1(+), Extends the Chronological Lifespan in Fission Yeast. FEMS Yeast Res. 2008;8(4):520-30. PubMed PMID: 18422613.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel gene, ecl1(+), extends the chronological lifespan in fission yeast. AU - Ohtsuka,Hokuto, AU - Mita,Satoka, AU - Ogawa,Yuki, AU - Azuma,Kenko, AU - Ito,Hirokazu, AU - Aiba,Hirofumi, Y1 - 2008/04/16/ PY - 2008/4/22/pubmed PY - 2008/7/17/medline PY - 2008/4/22/entrez SP - 520 EP - 30 JF - FEMS yeast research JO - FEMS Yeast Res VL - 8 IS - 4 N2 - We have identified a novel gene from Schizosaccharomyces pombe that we have named ecl1(+) (extender of the chronological lifespan). When ecl1(+) is provided on a high-copy number plasmid, it extends the viability of both the Deltasty1 MAP kinase mutant and the wild-type cells after entry into the stationary phase. ecl1(+) encodes an 80-amino acid polypeptide that had not been annotated in the current database. The ecl1(+)-mRNA increases transiently when the growth phase is changed from the log phase to the stationary phase. The Ecl1 protein is localized in the nucleus. Calorie restriction extends the chronological lifespan of wild-type and Deltaecl1 cells but not ecl1(+)-overproducing cells. The Deltapka1 mutant shows little, if any, additional extension of viability when Ecl1 is overproduced. The ste11(+) gene that is negatively controlled by Pka1 is up regulated when Ecl1 is overproduced. From these results we propose that the effect of Ecl1 overproduction may be mainly linked to and negatively affects the Pka1-dependent pathway. SN - 1567-1356 UR - https://www.unboundmedicine.com/medline/citation/18422613/A_novel_gene_ecl1_+__extends_the_chronological_lifespan_in_fission_yeast_ L2 - https://academic.oup.com/femsyr/article-lookup/doi/10.1111/j.1567-1364.2008.00379.x DB - PRIME DP - Unbound Medicine ER -