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Efficacy of in-feed probiotics against Aeromonas bestiarum and Ichthyophthirius multifiliis skin infections in rainbow trout (Oncorhynchus mykiss, Walbaum).
J Appl Microbiol. 2008 Sep; 105(3):723-32.JA

Abstract

AIMS

The aim of this study was to assess the efficacy of in-feed probiotics as a preventive measure against skin infections caused by Aeromonas bestiarum and Ichthyophthirius multifiliis (Ich) in rainbow trout.

METHODS AND RESULTS

Fin rot was induced in fish by intradermal injection with 0.1 ml volumes containing 10(5) cells per ml A. bestiarum at the base of the dorsal fin. Ich infections resulted from immersion in Ich-contaminated water. Each probiotic was administered orally [10(8) cells per g feed for GC2 (Aeromonas sobria) and 10(10) cells per g feed for BA211 (Brochothrix thermosphacta)] for 14 days. Results showed that, after challenge with A. bestiarum, probiotics GC2 and BA211 led to 76% and 88% survival, respectively, in contrast to 22% survival for controls. Fish fed with probiotic GC2 had 100% survival after challenge with Ich compared with 2% for probiotic BA211 and 0% for controls. Analysis of innate immune responses revealed that probiotic GC2 promoted higher phagocytic activity, whereas probiotic BA211 led to enhanced respiratory burst activity.

CONCLUSION

Of the two probiotics examined, GC2 was more effective in protecting against both fin rot and Ich. Each probiotic appeared to stimulate different pathways within the innate immune system.

SIGNIFICANCE AND IMPACT OF THE STUDY

This is the first demonstration that probiotics can protect fish against surface infections. Furthermore, this is the first time a probiotic has been shown to protect against a eucaryotic pathogen, namely I. multifiliis.

Authors+Show Affiliations

School of Life Sciences, Heriot-Watt University, Edinburgh, UK.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18422955

Citation

Pieters, N, et al. "Efficacy of In-feed Probiotics Against Aeromonas Bestiarum and Ichthyophthirius Multifiliis Skin Infections in Rainbow Trout (Oncorhynchus Mykiss, Walbaum)." Journal of Applied Microbiology, vol. 105, no. 3, 2008, pp. 723-32.
Pieters N, Brunt J, Austin B, et al. Efficacy of in-feed probiotics against Aeromonas bestiarum and Ichthyophthirius multifiliis skin infections in rainbow trout (Oncorhynchus mykiss, Walbaum). J Appl Microbiol. 2008;105(3):723-32.
Pieters, N., Brunt, J., Austin, B., & Lyndon, A. R. (2008). Efficacy of in-feed probiotics against Aeromonas bestiarum and Ichthyophthirius multifiliis skin infections in rainbow trout (Oncorhynchus mykiss, Walbaum). Journal of Applied Microbiology, 105(3), 723-32. https://doi.org/10.1111/j.1365-2672.2008.03817.x
Pieters N, et al. Efficacy of In-feed Probiotics Against Aeromonas Bestiarum and Ichthyophthirius Multifiliis Skin Infections in Rainbow Trout (Oncorhynchus Mykiss, Walbaum). J Appl Microbiol. 2008;105(3):723-32. PubMed PMID: 18422955.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Efficacy of in-feed probiotics against Aeromonas bestiarum and Ichthyophthirius multifiliis skin infections in rainbow trout (Oncorhynchus mykiss, Walbaum). AU - Pieters,N, AU - Brunt,J, AU - Austin,B, AU - Lyndon,A R, Y1 - 2008/04/18/ PY - 2008/4/22/pubmed PY - 2008/12/17/medline PY - 2008/4/22/entrez SP - 723 EP - 32 JF - Journal of applied microbiology JO - J Appl Microbiol VL - 105 IS - 3 N2 - AIMS: The aim of this study was to assess the efficacy of in-feed probiotics as a preventive measure against skin infections caused by Aeromonas bestiarum and Ichthyophthirius multifiliis (Ich) in rainbow trout. METHODS AND RESULTS: Fin rot was induced in fish by intradermal injection with 0.1 ml volumes containing 10(5) cells per ml A. bestiarum at the base of the dorsal fin. Ich infections resulted from immersion in Ich-contaminated water. Each probiotic was administered orally [10(8) cells per g feed for GC2 (Aeromonas sobria) and 10(10) cells per g feed for BA211 (Brochothrix thermosphacta)] for 14 days. Results showed that, after challenge with A. bestiarum, probiotics GC2 and BA211 led to 76% and 88% survival, respectively, in contrast to 22% survival for controls. Fish fed with probiotic GC2 had 100% survival after challenge with Ich compared with 2% for probiotic BA211 and 0% for controls. Analysis of innate immune responses revealed that probiotic GC2 promoted higher phagocytic activity, whereas probiotic BA211 led to enhanced respiratory burst activity. CONCLUSION: Of the two probiotics examined, GC2 was more effective in protecting against both fin rot and Ich. Each probiotic appeared to stimulate different pathways within the innate immune system. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first demonstration that probiotics can protect fish against surface infections. Furthermore, this is the first time a probiotic has been shown to protect against a eucaryotic pathogen, namely I. multifiliis. SN - 1365-2672 UR - https://www.unboundmedicine.com/medline/citation/18422955/Efficacy_of_in_feed_probiotics_against_Aeromonas_bestiarum_and_Ichthyophthirius_multifiliis_skin_infections_in_rainbow_trout__Oncorhynchus_mykiss_Walbaum__ L2 - https://doi.org/10.1111/j.1365-2672.2008.03817.x DB - PRIME DP - Unbound Medicine ER -