TY - JOUR T1 - Plasminogen activator inhibitor-1 (PAI-1) 4G/5G polymorphism and endometriosis. Influence of PAI-1 polymorphism on PAI-1 antigen and mRNA expression. AU - Ramón,Luis A, AU - Gilabert-Estellés,Juan, AU - Cosín,Raul, AU - Gilabert,Juan, AU - España,Francisco, AU - Castelló,Remedios, AU - Chirivella,Melitina, AU - Romeu,Alberto, AU - Estellés,Amparo, Y1 - 2008/04/18/ PY - 2008/01/09/received PY - 2008/02/06/revised PY - 2008/02/07/accepted PY - 2008/4/22/pubmed PY - 2008/12/17/medline PY - 2008/4/22/entrez SP - 854 EP - 60 JF - Thrombosis research JO - Thromb Res VL - 122 IS - 6 N2 - INTRODUCTION: Endometriosis is a benign gynecologic disease with a high prevalence. It is a multifactorial and polygenic entity in which the fibrinolytic system may be implicated. The objective of this study was to evaluate the plasminogen activator inhibitor-1 (PAI-1) 4G/5G polymorphism in a group of women with and without endometriosis and to analyze the influence of this polymorphism in PAI-1 expression in endometrial tissue and peritoneal fluid. MATERIAL AND METHODS: In 389 women (170 patients with endometriosis and 219 controls) PAI-1 4G/5G polymorphism was determined by PCR amplification using allele-specific primers. Quantitative real-time RT-PCR assay was used to quantify PAI-1 mRNA and PAI-1 antigen (ag) levels were quantified by ELISA. RESULTS: The genotype and allele frequencies of PAI-1 4G/5G polymorphism did not differ significantly between patients and controls. Control women with the 4G/4G genotype had higher endometrial PAI-1ag (P=0.026) and mRNA (P=0.014) levels than those with the 5G/5G genotype. Control carrying the 4G/4G genotype tended to have higher peritoneal fluid PAI-1ag levels than those carrying the 5G/5G genotype. Moreover, PAI-1ag levels in peritoneal fluid were higher in patients than in controls (P=0.003). CONCLUSIONS: The PAI-1 genotype distribution was similar in patients and controls. PAI-1 levels in endometrial tissue and peritoneal fluid seem to be associated with PAI-1 4G/5G polymorphism in controls. The increased PAI-1ag levels observed in peritoneal fluid from patients could contribute to increase the peritoneal adhesions observed in endometriosis. SN - 0049-3848 UR - https://www.unboundmedicine.com/medline/citation/18423526/Plasminogen_activator_inhibitor_1__PAI_1__4G/5G_polymorphism_and_endometriosis__Influence_of_PAI_1_polymorphism_on_PAI_1_antigen_and_mRNA_expression_ DB - PRIME DP - Unbound Medicine ER -