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Expression of E1AF, an ets-oncogene transcription factor, highly correlates with malignant phenotype of malignant melanoma through up-regulation of the membrane-type-1 matrix metalloproteinase gene.
Oncol Rep. 2008 May; 19(5):1093-8.OR

Abstract

Matrix metalloproteinase (MMP) is closely involved in the degradation of extracellular matrix and confers invasive and metastatic potential to malignant tumors. MMP-2 is a type-IV collagenase secreted as a proenzyme that is activated on the surface of the tumor cell by membrane-type 1-MMP (MT1-MMP). MT1-MMP plays a critical role during tumor progression and metastasis. We investigated the expression levels of E1AF and MT1-MMP in malignant melanoma cell lines and specimens from patients in order to clarify the mechanisms responsible for the invasion and metastasis of malignant melanoma. High levels of E1AF and MT1-MMP mRNA expression were observed in melanoma cells by Northern blotting and real-time PCR. The expression level was highly correlated with an invasive potential determined by an in vitro invasion assay. The down-regulation of MT1-MMP was identified when E1AF was knocked down by RNA interference. These results suggest that E1AF plays a crucial role in the invasion and metastasis of malignant melanoma through up-regulating the MT1-MMP expression.

Authors+Show Affiliations

Department of Oral Pathology and Biology, Hokkaido University Graduate School of Dental Medicine, Sapporo 060-8586, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18425363

Citation

Hata, Hironobu, et al. "Expression of E1AF, an Ets-oncogene Transcription Factor, Highly Correlates With Malignant Phenotype of Malignant Melanoma Through Up-regulation of the Membrane-type-1 Matrix Metalloproteinase Gene." Oncology Reports, vol. 19, no. 5, 2008, pp. 1093-8.
Hata H, Kitamura T, Higashino F, et al. Expression of E1AF, an ets-oncogene transcription factor, highly correlates with malignant phenotype of malignant melanoma through up-regulation of the membrane-type-1 matrix metalloproteinase gene. Oncol Rep. 2008;19(5):1093-8.
Hata, H., Kitamura, T., Higashino, F., Hida, K., Yoshida, K., Ohiro, Y., Totsuka, Y., Kitagawa, Y., & Shindoh, M. (2008). Expression of E1AF, an ets-oncogene transcription factor, highly correlates with malignant phenotype of malignant melanoma through up-regulation of the membrane-type-1 matrix metalloproteinase gene. Oncology Reports, 19(5), 1093-8.
Hata H, et al. Expression of E1AF, an Ets-oncogene Transcription Factor, Highly Correlates With Malignant Phenotype of Malignant Melanoma Through Up-regulation of the Membrane-type-1 Matrix Metalloproteinase Gene. Oncol Rep. 2008;19(5):1093-8. PubMed PMID: 18425363.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of E1AF, an ets-oncogene transcription factor, highly correlates with malignant phenotype of malignant melanoma through up-regulation of the membrane-type-1 matrix metalloproteinase gene. AU - Hata,Hironobu, AU - Kitamura,Tetsuya, AU - Higashino,Fumihiro, AU - Hida,Kyoko, AU - Yoshida,Koichi, AU - Ohiro,Yoichi, AU - Totsuka,Yasunori, AU - Kitagawa,Yoshimasa, AU - Shindoh,Masanobu, PY - 2008/4/22/pubmed PY - 2008/7/23/medline PY - 2008/4/22/entrez SP - 1093 EP - 8 JF - Oncology reports JO - Oncol Rep VL - 19 IS - 5 N2 - Matrix metalloproteinase (MMP) is closely involved in the degradation of extracellular matrix and confers invasive and metastatic potential to malignant tumors. MMP-2 is a type-IV collagenase secreted as a proenzyme that is activated on the surface of the tumor cell by membrane-type 1-MMP (MT1-MMP). MT1-MMP plays a critical role during tumor progression and metastasis. We investigated the expression levels of E1AF and MT1-MMP in malignant melanoma cell lines and specimens from patients in order to clarify the mechanisms responsible for the invasion and metastasis of malignant melanoma. High levels of E1AF and MT1-MMP mRNA expression were observed in melanoma cells by Northern blotting and real-time PCR. The expression level was highly correlated with an invasive potential determined by an in vitro invasion assay. The down-regulation of MT1-MMP was identified when E1AF was knocked down by RNA interference. These results suggest that E1AF plays a crucial role in the invasion and metastasis of malignant melanoma through up-regulating the MT1-MMP expression. SN - 1021-335X UR - https://www.unboundmedicine.com/medline/citation/18425363/Expression_of_E1AF_an_ets_oncogene_transcription_factor_highly_correlates_with_malignant_phenotype_of_malignant_melanoma_through_up_regulation_of_the_membrane_type_1_matrix_metalloproteinase_gene_ L2 - http://www.spandidos-publications.com/or/19/5/1093 DB - PRIME DP - Unbound Medicine ER -