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Characterization of Atropa belladonna L. compounds by capillary electrophoresis-electrospray ionization-time of flight-mass spectrometry and capillary electrophoresis-electrospray ionization-ion trap-mass spectrometry.
Electrophoresis. 2008 May; 29(10):2112-6.E

Abstract

This short communication describes the characterization of seven tropane alkaloid compounds in Atropa belladonna L. Thus a rapid and easy CE-electrospray interface (ESI)-TOF-MS procedure is developed to analyze these compounds in a pharmaceutical preparations of A. belladonna L. leaf extract. Optimum electrophoretic separation is obtained using an alkaline solution of 60 mM ammonium acetate at pH 8.5 containing 5% isopropanol. Under the optimum CE-ESI-TOF-MS conditions several important compounds such as tropine, belladonnine, norhyoscyamine, apoatropine, hyoscyamine, 6beta-hydroxyhyoscyamine, and scopolamine have been simultaneously identified from A. belladonna L. CE-ESI-IT-MS has been used to discriminate the putative presence of littorine. The sensitivity, together with mass accuracy and true isotopic pattern of the TOF-MS, allowed the identification of a broad series of tropane alkaloid compounds present in pharmaceutical preparations of A. belladonna L. leaf extract.

Authors+Show Affiliations

Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Granada, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18425749

Citation

Arráez-Román, David, et al. "Characterization of Atropa Belladonna L. Compounds By Capillary Electrophoresis-electrospray Ionization-time of Flight-mass Spectrometry and Capillary Electrophoresis-electrospray Ionization-ion Trap-mass Spectrometry." Electrophoresis, vol. 29, no. 10, 2008, pp. 2112-6.
Arráez-Román D, Zurek G, Bässmann C, et al. Characterization of Atropa belladonna L. compounds by capillary electrophoresis-electrospray ionization-time of flight-mass spectrometry and capillary electrophoresis-electrospray ionization-ion trap-mass spectrometry. Electrophoresis. 2008;29(10):2112-6.
Arráez-Román, D., Zurek, G., Bässmann, C., Segura-Carretero, A., & Fernández-Gutiérrez, A. (2008). Characterization of Atropa belladonna L. compounds by capillary electrophoresis-electrospray ionization-time of flight-mass spectrometry and capillary electrophoresis-electrospray ionization-ion trap-mass spectrometry. Electrophoresis, 29(10), 2112-6. https://doi.org/10.1002/elps.200700468
Arráez-Román D, et al. Characterization of Atropa Belladonna L. Compounds By Capillary Electrophoresis-electrospray Ionization-time of Flight-mass Spectrometry and Capillary Electrophoresis-electrospray Ionization-ion Trap-mass Spectrometry. Electrophoresis. 2008;29(10):2112-6. PubMed PMID: 18425749.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of Atropa belladonna L. compounds by capillary electrophoresis-electrospray ionization-time of flight-mass spectrometry and capillary electrophoresis-electrospray ionization-ion trap-mass spectrometry. AU - Arráez-Román,David, AU - Zurek,Gabriela, AU - Bässmann,Carsten, AU - Segura-Carretero,Antonio, AU - Fernández-Gutiérrez,Alberto, PY - 2008/4/22/pubmed PY - 2008/9/4/medline PY - 2008/4/22/entrez SP - 2112 EP - 6 JF - Electrophoresis JO - Electrophoresis VL - 29 IS - 10 N2 - This short communication describes the characterization of seven tropane alkaloid compounds in Atropa belladonna L. Thus a rapid and easy CE-electrospray interface (ESI)-TOF-MS procedure is developed to analyze these compounds in a pharmaceutical preparations of A. belladonna L. leaf extract. Optimum electrophoretic separation is obtained using an alkaline solution of 60 mM ammonium acetate at pH 8.5 containing 5% isopropanol. Under the optimum CE-ESI-TOF-MS conditions several important compounds such as tropine, belladonnine, norhyoscyamine, apoatropine, hyoscyamine, 6beta-hydroxyhyoscyamine, and scopolamine have been simultaneously identified from A. belladonna L. CE-ESI-IT-MS has been used to discriminate the putative presence of littorine. The sensitivity, together with mass accuracy and true isotopic pattern of the TOF-MS, allowed the identification of a broad series of tropane alkaloid compounds present in pharmaceutical preparations of A. belladonna L. leaf extract. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/18425749/Characterization_of_Atropa_belladonna_L__compounds_by_capillary_electrophoresis_electrospray_ionization_time_of_flight_mass_spectrometry_and_capillary_electrophoresis_electrospray_ionization_ion_trap_mass_spectrometry_ L2 - https://doi.org/10.1002/elps.200700468 DB - PRIME DP - Unbound Medicine ER -