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Analytical methods for the determination and mass spectrometric confirmation of 1-methyl-2-nitroso-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid and 2-nitroso-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid in foods.
Food Addit Contam. 1991 May-Jun; 8(3):275-89.FA

Abstract

A method is described for the determination of the two title compounds that is based on: (a) extraction of the acidified sample with methanol, (b) removal of fats and lipids by partitioning of the extract with n-hexane, (c) clean-up on acidic alumina extraction cartridge, and (d) determination by a post-HPLC column chemical denitrosation-thermal energy analyser (TEA) technique or by conventional HPLC-TEA analysis after derivatization of the compounds with diazomethane. Confirmation was carried out by HPLC-mass spectrometry of the free acids and also by gas chromatography-mass spectrometry of the methyl esters. The formation of both 1-methyl-2-nitroso-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid and 2-nitroso-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid in nitrosated samples of several Japanese and Chinese pickled vegetables, one soy sauce, and two cheeses was demonstrated.

Authors+Show Affiliations

Sen NP
Food Research Division, Health Protection Branch, Ottawa, Canada.
Seaman SW
No affiliation info available
Baddoo PA
No affiliation info available
Weber D
No affiliation info available
Malis G
No affiliation info available

MeSH

CarbolinesCarcinogensChromatography, High Pressure LiquidFood AnalysisGas Chromatography-Mass SpectrometryMass SpectrometryMutagensNitrosationNitroso CompoundsReproducibility of Results

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1842910

Citation

Sen, N P., et al. "Analytical Methods for the Determination and Mass Spectrometric Confirmation of 1-methyl-2-nitroso-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic Acid and 2-nitroso-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic Acid in Foods." Food Additives and Contaminants, vol. 8, no. 3, 1991, pp. 275-89.
Sen NP, Seaman SW, Baddoo PA, et al. Analytical methods for the determination and mass spectrometric confirmation of 1-methyl-2-nitroso-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid and 2-nitroso-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid in foods. Food Addit Contam. 1991;8(3):275-89.
Sen, N. P., Seaman, S. W., Baddoo, P. A., Weber, D., & Malis, G. (1991). Analytical methods for the determination and mass spectrometric confirmation of 1-methyl-2-nitroso-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid and 2-nitroso-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid in foods. Food Additives and Contaminants, 8(3), 275-89.
Sen NP, et al. Analytical Methods for the Determination and Mass Spectrometric Confirmation of 1-methyl-2-nitroso-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic Acid and 2-nitroso-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic Acid in Foods. Food Addit Contam. 1991 May-Jun;8(3):275-89. PubMed PMID: 1842910.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analytical methods for the determination and mass spectrometric confirmation of 1-methyl-2-nitroso-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid and 2-nitroso-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid in foods. AU - Sen,N P, AU - Seaman,S W, AU - Baddoo,P A, AU - Weber,D, AU - Malis,G, PY - 1991/5/1/pubmed PY - 1991/5/1/medline PY - 1991/5/1/entrez SP - 275 EP - 89 JF - Food additives and contaminants JO - Food Addit Contam VL - 8 IS - 3 N2 - A method is described for the determination of the two title compounds that is based on: (a) extraction of the acidified sample with methanol, (b) removal of fats and lipids by partitioning of the extract with n-hexane, (c) clean-up on acidic alumina extraction cartridge, and (d) determination by a post-HPLC column chemical denitrosation-thermal energy analyser (TEA) technique or by conventional HPLC-TEA analysis after derivatization of the compounds with diazomethane. Confirmation was carried out by HPLC-mass spectrometry of the free acids and also by gas chromatography-mass spectrometry of the methyl esters. The formation of both 1-methyl-2-nitroso-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid and 2-nitroso-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid in nitrosated samples of several Japanese and Chinese pickled vegetables, one soy sauce, and two cheeses was demonstrated. SN - 0265-203X UR - https://www.unboundmedicine.com/medline/citation/1842910/Analytical_methods_for_the_determination_and_mass_spectrometric_confirmation_of_1_methyl_2_nitroso_1234_tetrahydro_beta_carboline_3_carboxylic_acid_and_2_nitroso_1234_tetrahydro_beta_carboline_3_carboxylic_acid_in_foods_ DB - PRIME DP - Unbound Medicine ER -