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Pulsed electric field treatment as a potential method for microbial inactivation in scaffold materials for tissue engineering: the inactivation of bacteria in collagen gel.
J Appl Microbiol. 2008 Oct; 105(4):963-9.JA

Abstract

AIMS

To investigate the effectiveness of pulsed electric field (PEF) treatment as a new method for inactivation of micro-organisms in complex biomatrices and to assess this by quantifying the inactivation of Escherichia coli seeded in collagen gels.

METHODS AND RESULTS

PEF was applied to E. coli seeded collagen gels in static (nonflowing) chambers. The influence of electric field strength, pulse number and seeded cell densities were investigated. The highest level of inactivation was obtained at the maximum field strength of 45 kV cm(-1). For low levels of E. coli contamination (10(3) CFU ml(-1)), PEF treatment resulted in no viable E. coli being recovered from the gels. However, PEF treatment of gels containing higher cell densities (>or=10(4) CFU ml(-1)) did not achieve complete inactivation of E. coli.

CONCLUSIONS

PEF treatment successfully inactivated E. coli seeded in collagen gels by 3 log(10) CFU ml(-1). Complete inactivation was hindered at high cell densities by the tailing effect observed.

SIGNIFICANCE AND IMPACT OF THE STUDY

PEF shows potential as a novel, nondestructive method for decontamination of collagen-based matrices. Further investigation is required to ensure its compatibility with other proteins and therapeutic drugs for tissue engineering and drug delivery applications.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Griffiths S
Bioengineering Unit, University of Strathclyde, Glasgow, UK.
Smith S
No affiliation info available
MacGregor SJ
No affiliation info available
Anderson JG
No affiliation info available
van der Walle C
No affiliation info available
Beveridge JR
No affiliation info available
Helen Grant M
No affiliation info available

MeSH

CollagenColony Count, MicrobialElectric StimulationEquipment DesignEscherichia coliExtracellular MatrixGelsHumansMicrobial ViabilityTissue Scaffolds

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18429976

Citation

Griffiths, S, et al. "Pulsed Electric Field Treatment as a Potential Method for Microbial Inactivation in Scaffold Materials for Tissue Engineering: the Inactivation of Bacteria in Collagen Gel." Journal of Applied Microbiology, vol. 105, no. 4, 2008, pp. 963-9.
Griffiths S, Smith S, MacGregor SJ, et al. Pulsed electric field treatment as a potential method for microbial inactivation in scaffold materials for tissue engineering: the inactivation of bacteria in collagen gel. J Appl Microbiol. 2008;105(4):963-9.
Griffiths, S., Smith, S., MacGregor, S. J., Anderson, J. G., van der Walle, C., Beveridge, J. R., & Helen Grant, M. (2008). Pulsed electric field treatment as a potential method for microbial inactivation in scaffold materials for tissue engineering: the inactivation of bacteria in collagen gel. Journal of Applied Microbiology, 105(4), 963-9. https://doi.org/10.1111/j.1365-2672.2008.03829.x
Griffiths S, et al. Pulsed Electric Field Treatment as a Potential Method for Microbial Inactivation in Scaffold Materials for Tissue Engineering: the Inactivation of Bacteria in Collagen Gel. J Appl Microbiol. 2008;105(4):963-9. PubMed PMID: 18429976.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pulsed electric field treatment as a potential method for microbial inactivation in scaffold materials for tissue engineering: the inactivation of bacteria in collagen gel. AU - Griffiths,S, AU - Smith,S, AU - MacGregor,S J, AU - Anderson,J G, AU - van der Walle,C, AU - Beveridge,J R, AU - Helen Grant,M, Y1 - 2008/04/21/ PY - 2008/4/24/pubmed PY - 2008/12/25/medline PY - 2008/4/24/entrez SP - 963 EP - 9 JF - Journal of applied microbiology JO - J Appl Microbiol VL - 105 IS - 4 N2 - AIMS: To investigate the effectiveness of pulsed electric field (PEF) treatment as a new method for inactivation of micro-organisms in complex biomatrices and to assess this by quantifying the inactivation of Escherichia coli seeded in collagen gels. METHODS AND RESULTS: PEF was applied to E. coli seeded collagen gels in static (nonflowing) chambers. The influence of electric field strength, pulse number and seeded cell densities were investigated. The highest level of inactivation was obtained at the maximum field strength of 45 kV cm(-1). For low levels of E. coli contamination (10(3) CFU ml(-1)), PEF treatment resulted in no viable E. coli being recovered from the gels. However, PEF treatment of gels containing higher cell densities (>or=10(4) CFU ml(-1)) did not achieve complete inactivation of E. coli. CONCLUSIONS: PEF treatment successfully inactivated E. coli seeded in collagen gels by 3 log(10) CFU ml(-1). Complete inactivation was hindered at high cell densities by the tailing effect observed. SIGNIFICANCE AND IMPACT OF THE STUDY: PEF shows potential as a novel, nondestructive method for decontamination of collagen-based matrices. Further investigation is required to ensure its compatibility with other proteins and therapeutic drugs for tissue engineering and drug delivery applications. SN - 1365-2672 UR - https://www.unboundmedicine.com/medline/citation/18429976/Pulsed_electric_field_treatment_as_a_potential_method_for_microbial_inactivation_in_scaffold_materials_for_tissue_engineering:_the_inactivation_of_bacteria_in_collagen_gel_ DB - PRIME DP - Unbound Medicine ER -