Tags

Type your tag names separated by a space and hit enter

Possible involvement of nitric oxide (NO) signaling pathway in the antidepressant-like effect of MK-801(dizocilpine), a NMDA receptor antagonist in mouse forced swim test.
Indian J Exp Biol 2008; 46(3):164-70IJ

Abstract

L-arginine-nitric oxide (NO)-cyclic guanosine monophosphate (cGMP) is an important signaling pathway involved in depression. With this information, the present study aimed to study the involvement of this signaling pathway in the antidepressant-like action of MK-801 (dizocilpine; N-methyl-d-aspartate receptor antagonist) in the mouse forced-swim test. Total immobility period was recorded in mouse forced swim test for 6 min. MK-801 (5-25 microg/kg., ip) produced a U-shaped curve in reducing the immobility period. The antidepressant-like effect of MK-801 (10 microg/kg, ip) was prevented by pretreatment with L-arginine (750 mg/kg, ip) [substrate for nitric oxide synthase (NOS)]. Pretreatment of mice with 7-nitroindazole (7-NI) (25 mg/kg, ip) [a specific neuronal nitric oxide synthase inhibitor] produced potentiation of the action of subeffective dose of MK-801 (5 microg/kg, ip). In addition, treatment of mice with methylene blue (10 mg/kg, ip) [direct inhibitor of both nitric oxide synthase and soluble guanylate cyclase] potentiated the effect of MK-801 (5 microg/kg, ip) in the forced-swim test. Further, the reduction in the immobility period elicited by MK-801 (10 microg/kg, ip) was also inhibited by pretreatment with sildenafil (5 mg/kg, ip) [phosphodiesterase 5 inhibitor]. The various modulators used in the study and their combination did not produce any changes in locomotor activity per se and in combination with MK-801. MK-801 however, at higher doses (25 microg/kg, ip) produced hyperlocomotion. The results demonstrated the involvement of nitric oxide signaling pathway in the antidepressant-like effect of MK-801 in mouse forced-swim test.

Authors+Show Affiliations

Pharmacology Division, University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh 160014, India.No affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

18432055

Citation

Dhir, Ashish, and S K. Kulkarni. "Possible Involvement of Nitric Oxide (NO) Signaling Pathway in the Antidepressant-like Effect of MK-801(dizocilpine), a NMDA Receptor Antagonist in Mouse Forced Swim Test." Indian Journal of Experimental Biology, vol. 46, no. 3, 2008, pp. 164-70.
Dhir A, Kulkarni SK. Possible involvement of nitric oxide (NO) signaling pathway in the antidepressant-like effect of MK-801(dizocilpine), a NMDA receptor antagonist in mouse forced swim test. Indian J Exp Biol. 2008;46(3):164-70.
Dhir, A., & Kulkarni, S. K. (2008). Possible involvement of nitric oxide (NO) signaling pathway in the antidepressant-like effect of MK-801(dizocilpine), a NMDA receptor antagonist in mouse forced swim test. Indian Journal of Experimental Biology, 46(3), pp. 164-70.
Dhir A, Kulkarni SK. Possible Involvement of Nitric Oxide (NO) Signaling Pathway in the Antidepressant-like Effect of MK-801(dizocilpine), a NMDA Receptor Antagonist in Mouse Forced Swim Test. Indian J Exp Biol. 2008;46(3):164-70. PubMed PMID: 18432055.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Possible involvement of nitric oxide (NO) signaling pathway in the antidepressant-like effect of MK-801(dizocilpine), a NMDA receptor antagonist in mouse forced swim test. AU - Dhir,Ashish, AU - Kulkarni,S K, PY - 2008/4/25/pubmed PY - 2008/6/18/medline PY - 2008/4/25/entrez SP - 164 EP - 70 JF - Indian journal of experimental biology JO - Indian J. Exp. Biol. VL - 46 IS - 3 N2 - L-arginine-nitric oxide (NO)-cyclic guanosine monophosphate (cGMP) is an important signaling pathway involved in depression. With this information, the present study aimed to study the involvement of this signaling pathway in the antidepressant-like action of MK-801 (dizocilpine; N-methyl-d-aspartate receptor antagonist) in the mouse forced-swim test. Total immobility period was recorded in mouse forced swim test for 6 min. MK-801 (5-25 microg/kg., ip) produced a U-shaped curve in reducing the immobility period. The antidepressant-like effect of MK-801 (10 microg/kg, ip) was prevented by pretreatment with L-arginine (750 mg/kg, ip) [substrate for nitric oxide synthase (NOS)]. Pretreatment of mice with 7-nitroindazole (7-NI) (25 mg/kg, ip) [a specific neuronal nitric oxide synthase inhibitor] produced potentiation of the action of subeffective dose of MK-801 (5 microg/kg, ip). In addition, treatment of mice with methylene blue (10 mg/kg, ip) [direct inhibitor of both nitric oxide synthase and soluble guanylate cyclase] potentiated the effect of MK-801 (5 microg/kg, ip) in the forced-swim test. Further, the reduction in the immobility period elicited by MK-801 (10 microg/kg, ip) was also inhibited by pretreatment with sildenafil (5 mg/kg, ip) [phosphodiesterase 5 inhibitor]. The various modulators used in the study and their combination did not produce any changes in locomotor activity per se and in combination with MK-801. MK-801 however, at higher doses (25 microg/kg, ip) produced hyperlocomotion. The results demonstrated the involvement of nitric oxide signaling pathway in the antidepressant-like effect of MK-801 in mouse forced-swim test. SN - 0019-5189 UR - https://www.unboundmedicine.com/medline/citation/18432055/Possible_involvement_of_nitric_oxide__NO__signaling_pathway_in_the_antidepressant_like_effect_of_MK_801_dizocilpine__a_NMDA_receptor_antagonist_in_mouse_forced_swim_test_ L2 - https://medlineplus.gov/antidepressants.html DB - PRIME DP - Unbound Medicine ER -