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[Expression of VEGF protein and mRNA in neonatal rats with hyperoxia-induced lung injury].
Zhongguo Dang Dai Er Ke Za Zhi. 2008 Apr; 10(2):207-10.ZD

Abstract

OBJECTIVE

Vascular endothelial growth factor (VEGF) contributes to lung development and recovery of lung structure from lung injury. This study aimed to explore the changes of expression of VEGF protein and mRNA in neonatal rats following hyperoxic lung injury.

METHODS

Forty-eight Sprague-Dawley neonatal rats were randomly continually exposed to hyperoxia (FiO2=95%) or to room air (FiO2=21%, control group) 30 minutes after birth. VEGF protein and mRNA expression in the lungs was determined by immunohistochemical methods and reverse tanscription polymerasechain reaction (RT-PCR) respectively 3,7 and 14 days after birth.

RESULTS

VEGF protein and mRNA expression increased with increasing postnatal age in the control group. In the hyperoxia exposure group VEGF protein expression decreased markedly at 7 days (7.79+/-5.23 vs 12.67+/-3.82; P<0.01) and 14 days of hyperoxia exposure (5.85+/-3.37 vs 15.10+/-8.91; P<0.01) compared with the controls. VEGF mRNA expression in the hyperoxia exposure group was significantly reduced from 3 days (0.78+/-0.22 vs 1.19+/-0.63) through 14 days of hyperoxia exposure (0.48+/-0.12 vs 1.89+/-0.81) compared with the controls (P<0.01).

CONCLUSIONS

VEGF is associated with lung development in neonatal rats. Hyperoxia exposure can decrease VEGF protein and VEGF mRNA expression in the lungs of neonatal rats. VEGF might be involved in the pathogenesis of hyperoxic lung injury.

Authors+Show Affiliations

Department of Pediatrics, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China. houweidf@sina.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

18433549

Citation

Hou, Wei, et al. "[Expression of VEGF Protein and mRNA in Neonatal Rats With Hyperoxia-induced Lung Injury]." Zhongguo Dang Dai Er Ke Za Zhi = Chinese Journal of Contemporary Pediatrics, vol. 10, no. 2, 2008, pp. 207-10.
Hou W, Liu HY, Li D, et al. [Expression of VEGF protein and mRNA in neonatal rats with hyperoxia-induced lung injury]. Zhongguo Dang Dai Er Ke Za Zhi. 2008;10(2):207-10.
Hou, W., Liu, H. Y., Li, D., Zhou, J. P., & Chen, X. (2008). [Expression of VEGF protein and mRNA in neonatal rats with hyperoxia-induced lung injury]. Zhongguo Dang Dai Er Ke Za Zhi = Chinese Journal of Contemporary Pediatrics, 10(2), 207-10.
Hou W, et al. [Expression of VEGF Protein and mRNA in Neonatal Rats With Hyperoxia-induced Lung Injury]. Zhongguo Dang Dai Er Ke Za Zhi. 2008;10(2):207-10. PubMed PMID: 18433549.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Expression of VEGF protein and mRNA in neonatal rats with hyperoxia-induced lung injury]. AU - Hou,Wei, AU - Liu,Hai-Yan, AU - Li,Dan, AU - Zhou,Jian-Ping, AU - Chen,Xi, PY - 2008/4/25/pubmed PY - 2008/7/2/medline PY - 2008/4/25/entrez SP - 207 EP - 10 JF - Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics JO - Zhongguo Dang Dai Er Ke Za Zhi VL - 10 IS - 2 N2 - OBJECTIVE: Vascular endothelial growth factor (VEGF) contributes to lung development and recovery of lung structure from lung injury. This study aimed to explore the changes of expression of VEGF protein and mRNA in neonatal rats following hyperoxic lung injury. METHODS: Forty-eight Sprague-Dawley neonatal rats were randomly continually exposed to hyperoxia (FiO2=95%) or to room air (FiO2=21%, control group) 30 minutes after birth. VEGF protein and mRNA expression in the lungs was determined by immunohistochemical methods and reverse tanscription polymerasechain reaction (RT-PCR) respectively 3,7 and 14 days after birth. RESULTS: VEGF protein and mRNA expression increased with increasing postnatal age in the control group. In the hyperoxia exposure group VEGF protein expression decreased markedly at 7 days (7.79+/-5.23 vs 12.67+/-3.82; P<0.01) and 14 days of hyperoxia exposure (5.85+/-3.37 vs 15.10+/-8.91; P<0.01) compared with the controls. VEGF mRNA expression in the hyperoxia exposure group was significantly reduced from 3 days (0.78+/-0.22 vs 1.19+/-0.63) through 14 days of hyperoxia exposure (0.48+/-0.12 vs 1.89+/-0.81) compared with the controls (P<0.01). CONCLUSIONS: VEGF is associated with lung development in neonatal rats. Hyperoxia exposure can decrease VEGF protein and VEGF mRNA expression in the lungs of neonatal rats. VEGF might be involved in the pathogenesis of hyperoxic lung injury. SN - 1008-8830 UR - https://www.unboundmedicine.com/medline/citation/18433549/[Expression_of_VEGF_protein_and_mRNA_in_neonatal_rats_with_hyperoxia_induced_lung_injury]_ L2 - https://medlineplus.gov/lungdiseases.html DB - PRIME DP - Unbound Medicine ER -