Tags

Type your tag names separated by a space and hit enter

Transcriptional profiling of human skin-resident Langerhans cells and CD1a+ dermal dendritic cells: differential activation states suggest distinct functions.
J Leukoc Biol. 2008 Jul; 84(1):143-51.JL

Abstract

In human skin, two main populations of dendritic cells (DC) can be discriminated: dermal DC (DDC) and epidermal Langerhans cells (LC). Although extensively studied, most of the knowledge about DDC and LC phenotype and function is obtained from studying DDC and LC cultured in vitro or DDC and LC migrated from skin explants. These studies have left the exact relationship between steady-state human LC and DDC unclear: in particular, whether CD1a+ DDC represent migrated LC or whether they constitute a separate subset. To gain further insight in the kinship between skin-resident CD1a+ DDC and LC, we analyzed CD1a+ DDC and LC, isolated from steady-state skin samples, by high-density microarray analysis. Results show that the CD1a+ DDC specifically express markers associated with DDC phenotype, such as the macrophage mannose receptor, DC-specific ICAM-grabbing nonintegrin, the scavenger receptor CD36, coagulation factor XIIIa, and chemokine receptor CCR5, whereas LC specifically express Langerin, membrane ATPase (CD39), and CCR6, all hallmarks of the LC lineage. In addition, under steady-state conditions, both DC subsets display a strikingly different activation status, indicative of distinct functional properties. CD1a+ DDC exhibit a more activated, proinflammatory, migratory, and T cell-stimulatory profile, as compared with LC, whereas LC mainly express molecules involved in cell adhesion and DC retention in the epidermis. In conclusion, transcriptional profiling is consistent with the notion that CD1a+ DDC and LC represent two distinct DC subsets but also that under steady-state conditions, CD1a+ DDC and epidermal LC represent opposites of the DC activation spectrum.

Authors+Show Affiliations

Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18436579

Citation

Santegoets, Saskia J A M., et al. "Transcriptional Profiling of Human Skin-resident Langerhans Cells and CD1a+ Dermal Dendritic Cells: Differential Activation States Suggest Distinct Functions." Journal of Leukocyte Biology, vol. 84, no. 1, 2008, pp. 143-51.
Santegoets SJ, Gibbs S, Kroeze K, et al. Transcriptional profiling of human skin-resident Langerhans cells and CD1a+ dermal dendritic cells: differential activation states suggest distinct functions. J Leukoc Biol. 2008;84(1):143-51.
Santegoets, S. J., Gibbs, S., Kroeze, K., van de Ven, R., Scheper, R. J., Borrebaeck, C. A., de Gruijl, T. D., & Lindstedt, M. (2008). Transcriptional profiling of human skin-resident Langerhans cells and CD1a+ dermal dendritic cells: differential activation states suggest distinct functions. Journal of Leukocyte Biology, 84(1), 143-51. https://doi.org/10.1189/jlb.1107750
Santegoets SJ, et al. Transcriptional Profiling of Human Skin-resident Langerhans Cells and CD1a+ Dermal Dendritic Cells: Differential Activation States Suggest Distinct Functions. J Leukoc Biol. 2008;84(1):143-51. PubMed PMID: 18436579.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptional profiling of human skin-resident Langerhans cells and CD1a+ dermal dendritic cells: differential activation states suggest distinct functions. AU - Santegoets,Saskia J A M, AU - Gibbs,Susan, AU - Kroeze,Kim, AU - van de Ven,Rieneke, AU - Scheper,Rik J, AU - Borrebaeck,Carl A, AU - de Gruijl,Tanja D, AU - Lindstedt,Malin, Y1 - 2008/04/24/ PY - 2008/4/26/pubmed PY - 2008/8/9/medline PY - 2008/4/26/entrez SP - 143 EP - 51 JF - Journal of leukocyte biology JO - J Leukoc Biol VL - 84 IS - 1 N2 - In human skin, two main populations of dendritic cells (DC) can be discriminated: dermal DC (DDC) and epidermal Langerhans cells (LC). Although extensively studied, most of the knowledge about DDC and LC phenotype and function is obtained from studying DDC and LC cultured in vitro or DDC and LC migrated from skin explants. These studies have left the exact relationship between steady-state human LC and DDC unclear: in particular, whether CD1a+ DDC represent migrated LC or whether they constitute a separate subset. To gain further insight in the kinship between skin-resident CD1a+ DDC and LC, we analyzed CD1a+ DDC and LC, isolated from steady-state skin samples, by high-density microarray analysis. Results show that the CD1a+ DDC specifically express markers associated with DDC phenotype, such as the macrophage mannose receptor, DC-specific ICAM-grabbing nonintegrin, the scavenger receptor CD36, coagulation factor XIIIa, and chemokine receptor CCR5, whereas LC specifically express Langerin, membrane ATPase (CD39), and CCR6, all hallmarks of the LC lineage. In addition, under steady-state conditions, both DC subsets display a strikingly different activation status, indicative of distinct functional properties. CD1a+ DDC exhibit a more activated, proinflammatory, migratory, and T cell-stimulatory profile, as compared with LC, whereas LC mainly express molecules involved in cell adhesion and DC retention in the epidermis. In conclusion, transcriptional profiling is consistent with the notion that CD1a+ DDC and LC represent two distinct DC subsets but also that under steady-state conditions, CD1a+ DDC and epidermal LC represent opposites of the DC activation spectrum. SN - 0741-5400 UR - https://www.unboundmedicine.com/medline/citation/18436579/Transcriptional_profiling_of_human_skin_resident_Langerhans_cells_and_CD1a+_dermal_dendritic_cells:_differential_activation_states_suggest_distinct_functions_ L2 - https://doi.org/10.1189/jlb.1107750 DB - PRIME DP - Unbound Medicine ER -