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Identification of dermatophytes by sequence analysis of the rRNA gene internal transcribed spacer regions.
J Med Microbiol. 2008 May; 57(Pt 5):592-600.JM

Abstract

Identification of dermatophytes using the traditional method is sometimes problematic because of atypical microscopic or macroscopic morphology. The aim of this study was to evaluate the feasibility of using sequencing of the ribosomal internal transcribed spacer (ITS)1 and ITS2 regions for identification of 17 dermatophyte species. The ITS regions of 188 strains (62 reference strains and 126 clinical isolates) were amplified by PCR and sequenced. Species identification was made by sequence comparison with an in-house database comprising ITS sequences of type or neotype strains or by blast searches for homologous sequences in public databases. Strains producing discrepant results between conventional methods and ITS sequence analysis were analysed further by sequencing the D1-D2 domain of the large-subunit rRNA gene for species clarification. The identification rates by ITS1 and ITS2 sequencing were higher than 97 %. Based on reference sequences of type or neotype strains, it was noted that most strains of Trichophyton mentagrophytes were misidentifications of Trichophyton interdigitale. In addition, barcode sequences were present in species of the Microsporum canis complex and Trichophyton rubrum complex. These barcode sequences are useful for species delineation when the results of ITS sequencing are ambiguous. In conclusion, ITS sequencing provides a very accurate and useful method for the identification of dermatophytes.

Authors+Show Affiliations

Department of Medical Laboratory Science and Biotechnology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18436592

Citation

Li, Hsin Chieh, et al. "Identification of Dermatophytes By Sequence Analysis of the rRNA Gene Internal Transcribed Spacer Regions." Journal of Medical Microbiology, vol. 57, no. Pt 5, 2008, pp. 592-600.
Li HC, Bouchara JP, Hsu MM, et al. Identification of dermatophytes by sequence analysis of the rRNA gene internal transcribed spacer regions. J Med Microbiol. 2008;57(Pt 5):592-600.
Li, H. C., Bouchara, J. P., Hsu, M. M., Barton, R., Su, S., & Chang, T. C. (2008). Identification of dermatophytes by sequence analysis of the rRNA gene internal transcribed spacer regions. Journal of Medical Microbiology, 57(Pt 5), 592-600. https://doi.org/10.1099/jmm.0.47607-0
Li HC, et al. Identification of Dermatophytes By Sequence Analysis of the rRNA Gene Internal Transcribed Spacer Regions. J Med Microbiol. 2008;57(Pt 5):592-600. PubMed PMID: 18436592.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of dermatophytes by sequence analysis of the rRNA gene internal transcribed spacer regions. AU - Li,Hsin Chieh, AU - Bouchara,Jean-Philippe, AU - Hsu,Mark Ming-Long, AU - Barton,Richard, AU - Su,Shuli, AU - Chang,Tsung Chain, PY - 2008/4/26/pubmed PY - 2008/7/4/medline PY - 2008/4/26/entrez SP - 592 EP - 600 JF - Journal of medical microbiology JO - J. Med. Microbiol. VL - 57 IS - Pt 5 N2 - Identification of dermatophytes using the traditional method is sometimes problematic because of atypical microscopic or macroscopic morphology. The aim of this study was to evaluate the feasibility of using sequencing of the ribosomal internal transcribed spacer (ITS)1 and ITS2 regions for identification of 17 dermatophyte species. The ITS regions of 188 strains (62 reference strains and 126 clinical isolates) were amplified by PCR and sequenced. Species identification was made by sequence comparison with an in-house database comprising ITS sequences of type or neotype strains or by blast searches for homologous sequences in public databases. Strains producing discrepant results between conventional methods and ITS sequence analysis were analysed further by sequencing the D1-D2 domain of the large-subunit rRNA gene for species clarification. The identification rates by ITS1 and ITS2 sequencing were higher than 97 %. Based on reference sequences of type or neotype strains, it was noted that most strains of Trichophyton mentagrophytes were misidentifications of Trichophyton interdigitale. In addition, barcode sequences were present in species of the Microsporum canis complex and Trichophyton rubrum complex. These barcode sequences are useful for species delineation when the results of ITS sequencing are ambiguous. In conclusion, ITS sequencing provides a very accurate and useful method for the identification of dermatophytes. SN - 0022-2615 UR - https://www.unboundmedicine.com/medline/citation/18436592/Identification_of_dermatophytes_by_sequence_analysis_of_the_rRNA_gene_internal_transcribed_spacer_regions_ L2 - http://jmm.microbiologyresearch.org/pubmed/content/journal/jmm/10.1099/jmm.0.47607-0 DB - PRIME DP - Unbound Medicine ER -