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Effect of anticancer drugs on production of transforming growth factor and expression of p53 AND Bcl-2 proteins by MCF-7 and T47D cell lines of human breast carcinoma.

Abstract

AIM

To compare the capability of methotrexate, cisplatin, doxorubicine and vincristine to induce production of the transforming growth factor beta(1) (TGF-beta(1)) in two cell lines - MCF-7 and T47D - of human breast carcinoma, as well as to study sensitivity of these cells to TGF-beta(1) and mentioned anticancer drugs.

MATERIALS AND METHODS

ELISA for detection of TGF-beta content in conditioned culture media and Western-blot analysis of the proapoptotic p53 and antiapoptotic Bcl-2 proteins were applied.

RESULTS

T47D cells showing higher resistance to growth inhibiting effect of TGF-beta(1) were also refractory to cisplatin. There was no difference between MCF-7 and T47D cells in their sensitivity to methotrexate and doxorubicine, although T47D cells were more sensitive to vincristine. It was found that methotrexate and vincristine did not affect TGF-beta(1) production, while doxorubicine used at a dose of 1-100 ug/ml, significantly induced TGF-beta(1) production in both cell lines. p53 expression in T47D cells was higher than in MCF-7 cells where only doxorubicin induced strongly p53 expression. It should be noted, that Bcl-2 was better expressed in MCF-7 cells, while it was almost undetectable in T47D cells.

CONCLUSION

In cells of human mammary carcinoma of MCF-7 and T47D lines doxorubicine, unlike vincristine and methotrexate, in dose depending manner induces production of TGF-beta(1). TGF-beta(1) production in carcinoma cells was associated with doxorubicine-mediated p53 expression in MCF-7 cells or high basal level of p53 in T47D cells. The cells of MCF-7 line were more sensitive to growth inhibition by exogenous TGF-beta(1) and to cisplatine action than T47D cells, but there was no difference between these cell lines in sensitivity to other anticancer drugs.

Authors+Show Affiliations

,

Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv, Ukraine. stoika@cellbiol.lviv.ua

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Source

Experimental oncology 30:1 2008 Mar pg 35-41

MeSH

Animals
Antineoplastic Agents
Breast Neoplasms
Carcinoma
Cell Line, Tumor
Cell Proliferation
Cell Survival
Cisplatin
Doxorubicin
Humans
Jurkat Cells
Methotrexate
Mice
Proto-Oncogene Proteins c-bcl-2
Transforming Growth Factor beta1
Tumor Suppressor Protein p53
Vincristine

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18438339

Citation

Stoika, R S., et al. "Effect of Anticancer Drugs On Production of Transforming Growth Factor and Expression of P53 and Bcl-2 Proteins By MCF-7 and T47D Cell Lines of Human Breast Carcinoma." Experimental Oncology, vol. 30, no. 1, 2008, pp. 35-41.
Stoika RS, Yakymovych IA, Kashchak NI, et al. Effect of anticancer drugs on production of transforming growth factor and expression of p53 AND Bcl-2 proteins by MCF-7 and T47D cell lines of human breast carcinoma. Exp Oncol. 2008;30(1):35-41.
Stoika, R. S., Yakymovych, I. A., Kashchak, N. I., Boyko, M. M., Korynevska, A. V., Klyuchyvska, O. Y., ... Vorontsova, A. L. (2008). Effect of anticancer drugs on production of transforming growth factor and expression of p53 AND Bcl-2 proteins by MCF-7 and T47D cell lines of human breast carcinoma. Experimental Oncology, 30(1), pp. 35-41.
Stoika RS, et al. Effect of Anticancer Drugs On Production of Transforming Growth Factor and Expression of P53 and Bcl-2 Proteins By MCF-7 and T47D Cell Lines of Human Breast Carcinoma. Exp Oncol. 2008;30(1):35-41. PubMed PMID: 18438339.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of anticancer drugs on production of transforming growth factor and expression of p53 AND Bcl-2 proteins by MCF-7 and T47D cell lines of human breast carcinoma. AU - Stoika,R S, AU - Yakymovych,I A, AU - Kashchak,N I, AU - Boyko,M M, AU - Korynevska,A V, AU - Klyuchyvska,O Yu, AU - Shafranska,G I, AU - Yakymovych,M Ya, AU - Zhylchuk,V Ye, AU - Kudryavets,Yu Y, AU - Vorontsova,A L, PY - 2008/4/29/pubmed PY - 2008/6/6/medline PY - 2008/4/29/entrez SP - 35 EP - 41 JF - Experimental oncology JO - Exp. Oncol. VL - 30 IS - 1 N2 - AIM: To compare the capability of methotrexate, cisplatin, doxorubicine and vincristine to induce production of the transforming growth factor beta(1) (TGF-beta(1)) in two cell lines - MCF-7 and T47D - of human breast carcinoma, as well as to study sensitivity of these cells to TGF-beta(1) and mentioned anticancer drugs. MATERIALS AND METHODS: ELISA for detection of TGF-beta content in conditioned culture media and Western-blot analysis of the proapoptotic p53 and antiapoptotic Bcl-2 proteins were applied. RESULTS: T47D cells showing higher resistance to growth inhibiting effect of TGF-beta(1) were also refractory to cisplatin. There was no difference between MCF-7 and T47D cells in their sensitivity to methotrexate and doxorubicine, although T47D cells were more sensitive to vincristine. It was found that methotrexate and vincristine did not affect TGF-beta(1) production, while doxorubicine used at a dose of 1-100 ug/ml, significantly induced TGF-beta(1) production in both cell lines. p53 expression in T47D cells was higher than in MCF-7 cells where only doxorubicin induced strongly p53 expression. It should be noted, that Bcl-2 was better expressed in MCF-7 cells, while it was almost undetectable in T47D cells. CONCLUSION: In cells of human mammary carcinoma of MCF-7 and T47D lines doxorubicine, unlike vincristine and methotrexate, in dose depending manner induces production of TGF-beta(1). TGF-beta(1) production in carcinoma cells was associated with doxorubicine-mediated p53 expression in MCF-7 cells or high basal level of p53 in T47D cells. The cells of MCF-7 line were more sensitive to growth inhibition by exogenous TGF-beta(1) and to cisplatine action than T47D cells, but there was no difference between these cell lines in sensitivity to other anticancer drugs. SN - 1812-9269 UR - https://www.unboundmedicine.com/medline/citation/18438339/Effect_of_anticancer_drugs_on_production_of_transforming_growth_factor_and_expression_of_p53_AND_Bcl_2_proteins_by_MCF_7_and_T47D_cell_lines_of_human_breast_carcinoma_ L2 - https://medlineplus.gov/breastcancer.html DB - PRIME DP - Unbound Medicine ER -