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Hepatic gene expression profiles in juvenile rainbow trout (Oncorhynchus mykiss) fed fishmeal or fish oil-free diets.
Br J Nutr 2008; 100(5):953-67BJ

Abstract

Reducing the reliance on fishery by-products as amino acid and fatty acid sources in feeds for farmed fish is a major objective today. We evaluated the effect of dietary fish oil or dietary fishmeal replacement by vegetable oils and plant proteins respectively through analysis of hepatic transcriptomes in rainbow trout (Oncorhynchus mykiss). Fish were fed right from first feeding with diets based on plant by-products before being killed. We analysed the hepatic gene profile using trout cDNA microarrays (9K). Our data showed that seventy-one and seventy-five genes were affected after fish oil and fishmeal replacement respectively. The major part of modified gene expression coding for proteins of the metabolic pathways was as follows: (i) a lower level of expression for genes of energy metabolism found in fish after fishmeal and fish oil replacement; (ii) a lower level of gene expression for fatty acid metabolism (biosynthesis) in fish fed with vegetable oils; (iii) a differential expression of actors of detoxification metabolism in trout fed with vegetable oils; (iv) a lower level of expression of genes involved in protein metabolism in fish fed with plant proteins. Overall, our data suggest that dietary fish oil replacement is linked to a decreased capacity of fatty acid biosynthesis (fatty acid synthase) and variation of detoxification metabolism (cytochrome P450s) whereas dietary fishmeal replacement may depress protein metabolism in the liver as reflected by glutamine synthetase.

Authors+Show Affiliations

INRA, UMR1067 Nutrition Aquaculture and Genomics, Pôle d'Hydrobiologie, CD 918, F-64310 Saint-Pée-sur-Nivelle, France. panserat@st-pee.inra.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18439330

Citation

Panserat, Stéphane, et al. "Hepatic Gene Expression Profiles in Juvenile Rainbow Trout (Oncorhynchus Mykiss) Fed Fishmeal or Fish Oil-free Diets." The British Journal of Nutrition, vol. 100, no. 5, 2008, pp. 953-67.
Panserat S, Kolditz C, Richard N, et al. Hepatic gene expression profiles in juvenile rainbow trout (Oncorhynchus mykiss) fed fishmeal or fish oil-free diets. Br J Nutr. 2008;100(5):953-67.
Panserat, S., Kolditz, C., Richard, N., Plagnes-Juan, E., Piumi, F., Esquerré, D., ... Kaushik, S. (2008). Hepatic gene expression profiles in juvenile rainbow trout (Oncorhynchus mykiss) fed fishmeal or fish oil-free diets. The British Journal of Nutrition, 100(5), pp. 953-67. doi:10.1017/S0007114508981411.
Panserat S, et al. Hepatic Gene Expression Profiles in Juvenile Rainbow Trout (Oncorhynchus Mykiss) Fed Fishmeal or Fish Oil-free Diets. Br J Nutr. 2008;100(5):953-67. PubMed PMID: 18439330.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hepatic gene expression profiles in juvenile rainbow trout (Oncorhynchus mykiss) fed fishmeal or fish oil-free diets. AU - Panserat,Stéphane, AU - Kolditz,Cathy, AU - Richard,Nadège, AU - Plagnes-Juan,Elisabeth, AU - Piumi,Francois, AU - Esquerré,Diane, AU - Médale,Francoise, AU - Corraze,Genevieve, AU - Kaushik,Sadavisam, Y1 - 2008/04/28/ PY - 2008/4/29/pubmed PY - 2008/11/19/medline PY - 2008/4/29/entrez SP - 953 EP - 67 JF - The British journal of nutrition JO - Br. J. Nutr. VL - 100 IS - 5 N2 - Reducing the reliance on fishery by-products as amino acid and fatty acid sources in feeds for farmed fish is a major objective today. We evaluated the effect of dietary fish oil or dietary fishmeal replacement by vegetable oils and plant proteins respectively through analysis of hepatic transcriptomes in rainbow trout (Oncorhynchus mykiss). Fish were fed right from first feeding with diets based on plant by-products before being killed. We analysed the hepatic gene profile using trout cDNA microarrays (9K). Our data showed that seventy-one and seventy-five genes were affected after fish oil and fishmeal replacement respectively. The major part of modified gene expression coding for proteins of the metabolic pathways was as follows: (i) a lower level of expression for genes of energy metabolism found in fish after fishmeal and fish oil replacement; (ii) a lower level of gene expression for fatty acid metabolism (biosynthesis) in fish fed with vegetable oils; (iii) a differential expression of actors of detoxification metabolism in trout fed with vegetable oils; (iv) a lower level of expression of genes involved in protein metabolism in fish fed with plant proteins. Overall, our data suggest that dietary fish oil replacement is linked to a decreased capacity of fatty acid biosynthesis (fatty acid synthase) and variation of detoxification metabolism (cytochrome P450s) whereas dietary fishmeal replacement may depress protein metabolism in the liver as reflected by glutamine synthetase. SN - 1475-2662 UR - https://www.unboundmedicine.com/medline/citation/18439330/Hepatic_gene_expression_profiles_in_juvenile_rainbow_trout__Oncorhynchus_mykiss__fed_fishmeal_or_fish_oil_free_diets_ L2 - https://www.cambridge.org/core/product/identifier/S0007114508981411/type/journal_article DB - PRIME DP - Unbound Medicine ER -