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Acute effects of N-(2-propylpentanoyl)urea on hippocampal amino acid neurotransmitters in pilocarpine-induced seizure in rats.
Epilepsy Res. 2008 May; 79(2-3):151-7.ER

Abstract

The present study aimed to investigate the anticonvulsant activity as well as the effects on the level of hippocampal amino acid neurotransmitters (glutamate, aspartate, glycine and GABA) of N-(2-propylpentanoyl)urea (VPU) in comparison to its parent compound, valproic acid (VPA). VPU was more potent than VPA, exhibiting the median effective dose (ED(50)) of 49 mg/kg in protecting rats against pilocarpine-induced seizure whereas the corresponding value for VPA was 322 mg/kg. In vivo microdialysis demonstrated that an intraperitoneal administration of pilocarpine induced a pronounced increment of hippocampal glutamate and aspartate whereas no significant change was observed on the level of glycine and GABA. Pretreatment with either VPU (50 and 100 mg/kg) or VPA (300 and 600 mg/kg) completely abolished pilocarpine-evoked increases in extracellular glutamate and aspartate. In addition, a statistically significant reduction was also observed on the level of GABA and glycine but less than a drastic reduction of glutamate and aspartate level. Based on the finding that VPU and VPA could protect the animals against pilocarpine-induced seizure it is suggested that the reduction of inhibitory amino acid neurotransmitters was comparatively minor and offset by a pronounced reduction of glutamate and aspartate. Therefore, like VPA, the finding that VPU could drastically reduce pilocarpine-induced increases in glutamate and aspartate should account, at least partly, for its anticonvulsant activity observed in pilocarpine-induced seizure in experimental animals. Some other mechanism than those being reported herein should be further investigated.

Authors+Show Affiliations

Inter-department of Physiology, Graduate School, Chulalongkorn University, Bangkok 10330 Thailand.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18439803

Citation

Khongsombat, O, et al. "Acute Effects of N-(2-propylpentanoyl)urea On Hippocampal Amino Acid Neurotransmitters in Pilocarpine-induced Seizure in Rats." Epilepsy Research, vol. 79, no. 2-3, 2008, pp. 151-7.
Khongsombat O, Watanabe H, Tantisira B, et al. Acute effects of N-(2-propylpentanoyl)urea on hippocampal amino acid neurotransmitters in pilocarpine-induced seizure in rats. Epilepsy Res. 2008;79(2-3):151-7.
Khongsombat, O., Watanabe, H., Tantisira, B., Patarapanich, C., & Tantisira, M. H. (2008). Acute effects of N-(2-propylpentanoyl)urea on hippocampal amino acid neurotransmitters in pilocarpine-induced seizure in rats. Epilepsy Research, 79(2-3), 151-7. https://doi.org/10.1016/j.eplepsyres.2008.01.005
Khongsombat O, et al. Acute Effects of N-(2-propylpentanoyl)urea On Hippocampal Amino Acid Neurotransmitters in Pilocarpine-induced Seizure in Rats. Epilepsy Res. 2008;79(2-3):151-7. PubMed PMID: 18439803.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Acute effects of N-(2-propylpentanoyl)urea on hippocampal amino acid neurotransmitters in pilocarpine-induced seizure in rats. AU - Khongsombat,O, AU - Watanabe,H, AU - Tantisira,B, AU - Patarapanich,C, AU - Tantisira,M H, Y1 - 2008/04/24/ PY - 2007/09/05/received PY - 2008/01/22/revised PY - 2008/01/28/accepted PY - 2008/4/29/pubmed PY - 2008/7/23/medline PY - 2008/4/29/entrez SP - 151 EP - 7 JF - Epilepsy research JO - Epilepsy Res VL - 79 IS - 2-3 N2 - The present study aimed to investigate the anticonvulsant activity as well as the effects on the level of hippocampal amino acid neurotransmitters (glutamate, aspartate, glycine and GABA) of N-(2-propylpentanoyl)urea (VPU) in comparison to its parent compound, valproic acid (VPA). VPU was more potent than VPA, exhibiting the median effective dose (ED(50)) of 49 mg/kg in protecting rats against pilocarpine-induced seizure whereas the corresponding value for VPA was 322 mg/kg. In vivo microdialysis demonstrated that an intraperitoneal administration of pilocarpine induced a pronounced increment of hippocampal glutamate and aspartate whereas no significant change was observed on the level of glycine and GABA. Pretreatment with either VPU (50 and 100 mg/kg) or VPA (300 and 600 mg/kg) completely abolished pilocarpine-evoked increases in extracellular glutamate and aspartate. In addition, a statistically significant reduction was also observed on the level of GABA and glycine but less than a drastic reduction of glutamate and aspartate level. Based on the finding that VPU and VPA could protect the animals against pilocarpine-induced seizure it is suggested that the reduction of inhibitory amino acid neurotransmitters was comparatively minor and offset by a pronounced reduction of glutamate and aspartate. Therefore, like VPA, the finding that VPU could drastically reduce pilocarpine-induced increases in glutamate and aspartate should account, at least partly, for its anticonvulsant activity observed in pilocarpine-induced seizure in experimental animals. Some other mechanism than those being reported herein should be further investigated. SN - 0920-1211 UR - https://www.unboundmedicine.com/medline/citation/18439803/Acute_effects_of_N__2_propylpentanoyl_urea_on_hippocampal_amino_acid_neurotransmitters_in_pilocarpine_induced_seizure_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0920-1211(08)00040-5 DB - PRIME DP - Unbound Medicine ER -