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A putative DEAD-box RNA-helicase is required for normal zoospore development in the late blight pathogen Phytophthora infestans.
Fungal Genet Biol. 2008 Jun; 45(6):954-62.FG

Abstract

The asexual multinucleated sporangia of Phytophthora infestans can germinate directly through a germ tube or indirectly by releasing zoospores. The molecular mechanisms controlling sporangial cytokinesis or sporangial cleavage, and zoospore release are largely unknown. Sporangial cleavage is initiated by a cold shock that eventually compartmentalizes single nuclei within each zoospore. Comparison of EST representation in different cDNA libraries revealed a putative ATP-dependent DEAD-box RNA-helicase gene in P. infestans, Pi-RNH1, which has a 140-fold increased expression level in young zoospores compared to uncleaved sporangia. RNA interference was employed to determine the role of Pi-RNH1 in zoospore development. Silencing efficiencies of up to 99% were achieved in some transiently-silenced lines. These Pi-RNH1-silenced lines produced large aberrant zoospores that had undergone partial cleavage and often had multiple flagella on their surface. Transmission electron microscopy revealed that cytoplasmic vesicles fused in the silenced lines, resulting in the formation of large vesicles. The Pi-RNH1-silenced zoospores were also sensitive to osmotic pressure and often ruptured upon release from the sporangia. These findings indicate that Pi-RNH1 has a major function in zoospore development and its potential role in cytokinesis is discussed.

Authors+Show Affiliations

Aberdeen Oomycete Group, College of Life Science and Medicine, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, Scotland, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18439859

Citation

Walker, Claire A., et al. "A Putative DEAD-box RNA-helicase Is Required for Normal Zoospore Development in the Late Blight Pathogen Phytophthora Infestans." Fungal Genetics and Biology : FG & B, vol. 45, no. 6, 2008, pp. 954-62.
Walker CA, Köppe M, Grenville-Briggs LJ, et al. A putative DEAD-box RNA-helicase is required for normal zoospore development in the late blight pathogen Phytophthora infestans. Fungal Genet Biol. 2008;45(6):954-62.
Walker, C. A., Köppe, M., Grenville-Briggs, L. J., Avrova, A. O., Horner, N. R., McKinnon, A. D., Whisson, S. C., Birch, P. R., & van West, P. (2008). A putative DEAD-box RNA-helicase is required for normal zoospore development in the late blight pathogen Phytophthora infestans. Fungal Genetics and Biology : FG & B, 45(6), 954-62. https://doi.org/10.1016/j.fgb.2008.03.004
Walker CA, et al. A Putative DEAD-box RNA-helicase Is Required for Normal Zoospore Development in the Late Blight Pathogen Phytophthora Infestans. Fungal Genet Biol. 2008;45(6):954-62. PubMed PMID: 18439859.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A putative DEAD-box RNA-helicase is required for normal zoospore development in the late blight pathogen Phytophthora infestans. AU - Walker,Claire A, AU - Köppe,Maico, AU - Grenville-Briggs,Laura J, AU - Avrova,Anna O, AU - Horner,Neil R, AU - McKinnon,Alastair D, AU - Whisson,Stephen C, AU - Birch,Paul R J, AU - van West,Pieter, Y1 - 2008/03/20/ PY - 2007/11/22/received PY - 2008/03/10/revised PY - 2008/03/12/accepted PY - 2008/4/29/pubmed PY - 2008/6/17/medline PY - 2008/4/29/entrez SP - 954 EP - 62 JF - Fungal genetics and biology : FG & B JO - Fungal Genet. Biol. VL - 45 IS - 6 N2 - The asexual multinucleated sporangia of Phytophthora infestans can germinate directly through a germ tube or indirectly by releasing zoospores. The molecular mechanisms controlling sporangial cytokinesis or sporangial cleavage, and zoospore release are largely unknown. Sporangial cleavage is initiated by a cold shock that eventually compartmentalizes single nuclei within each zoospore. Comparison of EST representation in different cDNA libraries revealed a putative ATP-dependent DEAD-box RNA-helicase gene in P. infestans, Pi-RNH1, which has a 140-fold increased expression level in young zoospores compared to uncleaved sporangia. RNA interference was employed to determine the role of Pi-RNH1 in zoospore development. Silencing efficiencies of up to 99% were achieved in some transiently-silenced lines. These Pi-RNH1-silenced lines produced large aberrant zoospores that had undergone partial cleavage and often had multiple flagella on their surface. Transmission electron microscopy revealed that cytoplasmic vesicles fused in the silenced lines, resulting in the formation of large vesicles. The Pi-RNH1-silenced zoospores were also sensitive to osmotic pressure and often ruptured upon release from the sporangia. These findings indicate that Pi-RNH1 has a major function in zoospore development and its potential role in cytokinesis is discussed. SN - 1096-0937 UR - https://www.unboundmedicine.com/medline/citation/18439859/A_putative_DEAD_box_RNA_helicase_is_required_for_normal_zoospore_development_in_the_late_blight_pathogen_Phytophthora_infestans_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1087-1845(08)00040-6 DB - PRIME DP - Unbound Medicine ER -