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Defective functions of circulating CD4+CD25+ and CD4+CD25- T cells in patients with chronic ordinary urticaria.
J Dermatol Sci. 2008 Aug; 51(2):121-30.JD

Abstract

BACKGROUND

Patients with chronic ordinary urticaria (CU) are divided into two groups: 30-50% have chronic autoimmune urticaria, and the remainder have chronic idiopathic urticaria. CD4(+)CD25(+) regulatory T (Treg) cells play critical roles in maintaining peripheral tolerance and preventing autoimmunity, but the characteristics of Treg cells have not yet been defined in CU.

OBJECTIVE

To identify whether CD4(+) T cells play an important immunoregulatory role in the etiology of CU, we determined the frequencies and functions of circulating CD4(+)CD25(+) and CD4(+)CD25(-) T cells in CU patients and healthy control subjects, with special focus on the characteristics of CD4(+)CD25(+) T cells.

METHODS

Peripheral blood mononuclear cells (PBMCs) were obtained from CU and healthy controls in this study. The frequency of CD4(+)CD25(+) T cells in PBMCs was detected by flow cytometry. The expression levels of forkhead box P3 (FOXP3) and transforming growth factor-beta (TGF-beta) in CD4(+)CD25(+) T cells were detected by real-time PCR. Furthermore, the suppressive function of CD4(+)CD25(+) T cells was analyzed. Additionally, the Th1/Th2 cytokine secretory profile in mitogen-stimulated CD4(+)CD25(-) T cells was measured by ELISA.

RESULTS

An increased frequency of CD4(+)CD25(+) T cells was observed in CU patients (n=19) compared to control subjects (n=7). No significant difference was detected in the expression levels of FOXP3 or TGF-beta between CU patients (n=14) and control subjects (n=7). Strikingly, the suppressive capacity of CD4(+)CD25(+) Treg cells from 2 of 5 CU patients was partially defective. We also found that cytokine production from CD4(+)CD25(-) T cells was significantly reduced in CU patients (n=9) compared to healthy donors (n=11).

CONCLUSIONS

Our data demonstrate that CD4(+)CD25(+) and CD4(+)CD25(-) T cells in PBMCs exhibit defective functions in CU patients.

Authors+Show Affiliations

Pediatrics, Cathay General Hospital, Taipei, Taiwan, ROC.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18440785

Citation

Chen, Wu-Charng, et al. "Defective Functions of Circulating CD4+CD25+ and CD4+CD25- T Cells in Patients With Chronic Ordinary Urticaria." Journal of Dermatological Science, vol. 51, no. 2, 2008, pp. 121-30.
Chen WC, Chiang BL, Liu HE, et al. Defective functions of circulating CD4+CD25+ and CD4+CD25- T cells in patients with chronic ordinary urticaria. J Dermatol Sci. 2008;51(2):121-30.
Chen, W. C., Chiang, B. L., Liu, H. E., Leu, S. J., & Lee, Y. L. (2008). Defective functions of circulating CD4+CD25+ and CD4+CD25- T cells in patients with chronic ordinary urticaria. Journal of Dermatological Science, 51(2), 121-30. https://doi.org/10.1016/j.jdermsci.2008.02.012
Chen WC, et al. Defective Functions of Circulating CD4+CD25+ and CD4+CD25- T Cells in Patients With Chronic Ordinary Urticaria. J Dermatol Sci. 2008;51(2):121-30. PubMed PMID: 18440785.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Defective functions of circulating CD4+CD25+ and CD4+CD25- T cells in patients with chronic ordinary urticaria. AU - Chen,Wu-Charng, AU - Chiang,Bor-Luen, AU - Liu,H Eugene, AU - Leu,Sy-Jye, AU - Lee,Yueh-Lun, Y1 - 2008/04/28/ PY - 2007/10/02/received PY - 2008/02/22/revised PY - 2008/02/27/accepted PY - 2008/4/29/pubmed PY - 2008/10/15/medline PY - 2008/4/29/entrez SP - 121 EP - 30 JF - Journal of dermatological science JO - J. Dermatol. Sci. VL - 51 IS - 2 N2 - BACKGROUND: Patients with chronic ordinary urticaria (CU) are divided into two groups: 30-50% have chronic autoimmune urticaria, and the remainder have chronic idiopathic urticaria. CD4(+)CD25(+) regulatory T (Treg) cells play critical roles in maintaining peripheral tolerance and preventing autoimmunity, but the characteristics of Treg cells have not yet been defined in CU. OBJECTIVE: To identify whether CD4(+) T cells play an important immunoregulatory role in the etiology of CU, we determined the frequencies and functions of circulating CD4(+)CD25(+) and CD4(+)CD25(-) T cells in CU patients and healthy control subjects, with special focus on the characteristics of CD4(+)CD25(+) T cells. METHODS: Peripheral blood mononuclear cells (PBMCs) were obtained from CU and healthy controls in this study. The frequency of CD4(+)CD25(+) T cells in PBMCs was detected by flow cytometry. The expression levels of forkhead box P3 (FOXP3) and transforming growth factor-beta (TGF-beta) in CD4(+)CD25(+) T cells were detected by real-time PCR. Furthermore, the suppressive function of CD4(+)CD25(+) T cells was analyzed. Additionally, the Th1/Th2 cytokine secretory profile in mitogen-stimulated CD4(+)CD25(-) T cells was measured by ELISA. RESULTS: An increased frequency of CD4(+)CD25(+) T cells was observed in CU patients (n=19) compared to control subjects (n=7). No significant difference was detected in the expression levels of FOXP3 or TGF-beta between CU patients (n=14) and control subjects (n=7). Strikingly, the suppressive capacity of CD4(+)CD25(+) Treg cells from 2 of 5 CU patients was partially defective. We also found that cytokine production from CD4(+)CD25(-) T cells was significantly reduced in CU patients (n=9) compared to healthy donors (n=11). CONCLUSIONS: Our data demonstrate that CD4(+)CD25(+) and CD4(+)CD25(-) T cells in PBMCs exhibit defective functions in CU patients. SN - 0923-1811 UR - https://www.unboundmedicine.com/medline/citation/18440785/Defective_functions_of_circulating_CD4+CD25+_and_CD4+CD25__T_cells_in_patients_with_chronic_ordinary_urticaria_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0923-1811(08)00082-0 DB - PRIME DP - Unbound Medicine ER -