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Absorption and biological activity of phytochemical-rich extracts from açai (Euterpe oleracea Mart.) pulp and oil in vitro.
J Agric Food Chem. 2008 May 28; 56(10):3593-600.JA

Abstract

Polyphenolic extracts from various fruits and vegetables have been shown to exert growth inhibitory effects in cell culture studies. Whereas individual polyphenolic compounds have been extensively evaluated, understanding of the biological activity of polyphenolic extracts from natural sources is limited and critical to the understanding of their potential effects on the human body. This study investigated the absorption and antiproliferative effects of phytochemical extracts from acai pulp and a polyphenolic-enriched acai oil obtained from the fruit pulp of the acai berry (Euterpe oleracea Mart.). Chemical composition, antioxidant properties, and polyphenolic absorption of phytochemical fractions in a Caco-2 monolayer were determined, along with their cytotoxicity in HT-29 human colon adenocarcinoma cells. Standardized extracts were characterized by their predominance of hydroxybenzoic acids, monomeric flavan-3-ols, and procyanidin dimers and trimers. Polyphenolic mixtures (0-12 microg of gallic acid equiv/mL) from both acai pulp and acai oil extracts inhibited cell proliferation by up to 90.7%, which was accompanied by an increase of up to 2.1-fold in reactive oxygen species. Absorption experiments using a Caco-2 intestinal cell monolayer demonstrated that phenolic acids such as p-hydroxybenzoic, vanillic, syringic, and ferulic acids, in the presence of DMSO, were readily transported from the apical to the basolateral side along with monomeric flavanols such as (+)-catechin and (-)-epicatechin. Results from this study provide further evidence for the bioactive properties of acai polyphenolics and offer new insight on their composition and cellular absorption.

Authors+Show Affiliations

Department of Nutrition and Food Science and Department of Veterinary Physiology and Pharmacology, Texas A&M University, College Station, Texas 77843, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18442253

Citation

Pacheco-Palencia, Lisbeth A., et al. "Absorption and Biological Activity of Phytochemical-rich Extracts From Açai (Euterpe Oleracea Mart.) Pulp and Oil in Vitro." Journal of Agricultural and Food Chemistry, vol. 56, no. 10, 2008, pp. 3593-600.
Pacheco-Palencia LA, Talcott ST, Safe S, et al. Absorption and biological activity of phytochemical-rich extracts from açai (Euterpe oleracea Mart.) pulp and oil in vitro. J Agric Food Chem. 2008;56(10):3593-600.
Pacheco-Palencia, L. A., Talcott, S. T., Safe, S., & Mertens-Talcott, S. (2008). Absorption and biological activity of phytochemical-rich extracts from açai (Euterpe oleracea Mart.) pulp and oil in vitro. Journal of Agricultural and Food Chemistry, 56(10), 3593-600. https://doi.org/10.1021/jf8001608
Pacheco-Palencia LA, et al. Absorption and Biological Activity of Phytochemical-rich Extracts From Açai (Euterpe Oleracea Mart.) Pulp and Oil in Vitro. J Agric Food Chem. 2008 May 28;56(10):3593-600. PubMed PMID: 18442253.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Absorption and biological activity of phytochemical-rich extracts from açai (Euterpe oleracea Mart.) pulp and oil in vitro. AU - Pacheco-Palencia,Lisbeth A, AU - Talcott,Stephen T, AU - Safe,Stephen, AU - Mertens-Talcott,Susanne, Y1 - 2008/04/29/ PY - 2008/4/30/pubmed PY - 2008/7/29/medline PY - 2008/4/30/entrez SP - 3593 EP - 600 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 56 IS - 10 N2 - Polyphenolic extracts from various fruits and vegetables have been shown to exert growth inhibitory effects in cell culture studies. Whereas individual polyphenolic compounds have been extensively evaluated, understanding of the biological activity of polyphenolic extracts from natural sources is limited and critical to the understanding of their potential effects on the human body. This study investigated the absorption and antiproliferative effects of phytochemical extracts from acai pulp and a polyphenolic-enriched acai oil obtained from the fruit pulp of the acai berry (Euterpe oleracea Mart.). Chemical composition, antioxidant properties, and polyphenolic absorption of phytochemical fractions in a Caco-2 monolayer were determined, along with their cytotoxicity in HT-29 human colon adenocarcinoma cells. Standardized extracts were characterized by their predominance of hydroxybenzoic acids, monomeric flavan-3-ols, and procyanidin dimers and trimers. Polyphenolic mixtures (0-12 microg of gallic acid equiv/mL) from both acai pulp and acai oil extracts inhibited cell proliferation by up to 90.7%, which was accompanied by an increase of up to 2.1-fold in reactive oxygen species. Absorption experiments using a Caco-2 intestinal cell monolayer demonstrated that phenolic acids such as p-hydroxybenzoic, vanillic, syringic, and ferulic acids, in the presence of DMSO, were readily transported from the apical to the basolateral side along with monomeric flavanols such as (+)-catechin and (-)-epicatechin. Results from this study provide further evidence for the bioactive properties of acai polyphenolics and offer new insight on their composition and cellular absorption. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/18442253/Absorption_and_biological_activity_of_phytochemical_rich_extracts_from_açai__Euterpe_oleracea_Mart___pulp_and_oil_in_vitro_ DB - PRIME DP - Unbound Medicine ER -