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Heat shock and ethanol stress provoke distinctly different responses in 3'-processing and nuclear export of HSP mRNA in Saccharomyces cerevisiae.
Biochem J. 2008 Aug 15; 414(1):111-9.BJ

Abstract

Under conditions of heat shock at 42 degrees C, mRNAs of HSP (heat shock protein) genes are exported out of the nucleus, whereas bulk poly(A)(+) (polyadenylated) mRNA shows a nuclear accumulation in Saccharomyces cerevisiae. Such a selective mRNA export seems an efficacious strategy of yeast cells to adapt rapidly to stress. Although ethanol stress (10%, v/v) as well as heat shock blocks the export of bulk poly(A)(+) mRNA, the differences and/or similarity between heat shock and ethanol stress in the mechanisms of selective mRNA export still remain to be clarified. We found that ethanol stress induced transcriptional activation of a subset of yeast HSP genes; however, intriguingly, most such transcripts remained in the nucleus in a hyperadenylated state and, as a consequence, were not translated into HSPs. Elimination of ethanol resulted in a rapid shortening of the poly(A) tails of HSP mRNAs, loss of their nuclear retention, and the coincidental synthesis of the respective HSPs. Since HSP mRNAs are selectively exported from the nucleus in heat-shocked cells, yeast cells respond differently to ethanol stress and heat shock in the 3'-processing and transport of HSP mRNAs. Furthermore, these results also suggest that hyperadenylation and nuclear retention of mRNAs might be used as a means to control eukaryotic gene expression under stressed conditions.

Authors+Show Affiliations

Laboratory of Molecular Microbiology, Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan. izawa@kais.kyoto-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18442359

Citation

Izawa, Shingo, et al. "Heat Shock and Ethanol Stress Provoke Distinctly Different Responses in 3'-processing and Nuclear Export of HSP mRNA in Saccharomyces Cerevisiae." The Biochemical Journal, vol. 414, no. 1, 2008, pp. 111-9.
Izawa S, Kita T, Ikeda K, et al. Heat shock and ethanol stress provoke distinctly different responses in 3'-processing and nuclear export of HSP mRNA in Saccharomyces cerevisiae. Biochem J. 2008;414(1):111-9.
Izawa, S., Kita, T., Ikeda, K., & Inoue, Y. (2008). Heat shock and ethanol stress provoke distinctly different responses in 3'-processing and nuclear export of HSP mRNA in Saccharomyces cerevisiae. The Biochemical Journal, 414(1), 111-9. https://doi.org/10.1042/BJ20071567
Izawa S, et al. Heat Shock and Ethanol Stress Provoke Distinctly Different Responses in 3'-processing and Nuclear Export of HSP mRNA in Saccharomyces Cerevisiae. Biochem J. 2008 Aug 15;414(1):111-9. PubMed PMID: 18442359.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Heat shock and ethanol stress provoke distinctly different responses in 3'-processing and nuclear export of HSP mRNA in Saccharomyces cerevisiae. AU - Izawa,Shingo, AU - Kita,Takeomi, AU - Ikeda,Kayo, AU - Inoue,Yoshiharu, PY - 2008/4/30/pubmed PY - 2008/8/19/medline PY - 2008/4/30/entrez SP - 111 EP - 9 JF - The Biochemical journal JO - Biochem J VL - 414 IS - 1 N2 - Under conditions of heat shock at 42 degrees C, mRNAs of HSP (heat shock protein) genes are exported out of the nucleus, whereas bulk poly(A)(+) (polyadenylated) mRNA shows a nuclear accumulation in Saccharomyces cerevisiae. Such a selective mRNA export seems an efficacious strategy of yeast cells to adapt rapidly to stress. Although ethanol stress (10%, v/v) as well as heat shock blocks the export of bulk poly(A)(+) mRNA, the differences and/or similarity between heat shock and ethanol stress in the mechanisms of selective mRNA export still remain to be clarified. We found that ethanol stress induced transcriptional activation of a subset of yeast HSP genes; however, intriguingly, most such transcripts remained in the nucleus in a hyperadenylated state and, as a consequence, were not translated into HSPs. Elimination of ethanol resulted in a rapid shortening of the poly(A) tails of HSP mRNAs, loss of their nuclear retention, and the coincidental synthesis of the respective HSPs. Since HSP mRNAs are selectively exported from the nucleus in heat-shocked cells, yeast cells respond differently to ethanol stress and heat shock in the 3'-processing and transport of HSP mRNAs. Furthermore, these results also suggest that hyperadenylation and nuclear retention of mRNAs might be used as a means to control eukaryotic gene expression under stressed conditions. SN - 1470-8728 UR - https://www.unboundmedicine.com/medline/citation/18442359/Heat_shock_and_ethanol_stress_provoke_distinctly_different_responses_in_3'_processing_and_nuclear_export_of_HSP_mRNA_in_Saccharomyces_cerevisiae_ L2 - https://portlandpress.com/biochemj/article-lookup/doi/10.1042/BJ20071567 DB - PRIME DP - Unbound Medicine ER -