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Involvement of mitogen-activated protein kinases and nuclear factor-kappa B activation in nitric oxide-induced interleukin-8 expression in human pulp cells.

Abstract

OBJECTIVE

This study examined the effect of nitric oxide (NO) on interleukin-8 (IL-8) production and the involvement of mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-kappaB) signaling pathways in primary cultured human pulp cells.

STUDY DESIGN

IL-8 production was measured using enzyme-linked immunosorbent assay (ELISA) and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. MAPK activation and IkappaB degradation and phosphorylation were determined by western blotting.

RESULTS

Sodium nitroprusside (SNP), an NO donor, has increased IL-8 secretion and mRNA expression in a dose- and time-dependent manner. SNP induced the phosphorylation of p38 MAPK and extracellular-regulated kinase (ERK), degradation and phosphorylation of IkappaB, and activation of NF-kappaB. Furthermore, inhibition of the ERK, p38, and NF-kappaB pathways blocked SNP-induced IL-8 secretion.

CONCLUSION

Human pulp cells showed NO-induced IL-8 expression via the MAPK and NF-kappaB pathways, which may play an important role in the inflammatory responses of pulp and periapical lesions.

Authors+Show Affiliations

Department of Conservative Dentistry, College of Dentistry, Wonkwang University, Iksan, South Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18442745

Citation

Min, Kyung-San, et al. "Involvement of Mitogen-activated Protein Kinases and Nuclear Factor-kappa B Activation in Nitric Oxide-induced Interleukin-8 Expression in Human Pulp Cells." Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics, vol. 105, no. 5, 2008, pp. 654-60.
Min KS, Kim HI, Chang HS, et al. Involvement of mitogen-activated protein kinases and nuclear factor-kappa B activation in nitric oxide-induced interleukin-8 expression in human pulp cells. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008;105(5):654-60.
Min, K. S., Kim, H. I., Chang, H. S., Kim, H. R., Pae, H. O., Chung, H. T., Hong, S. H., Shin, H. I., Hong, C. U., Lee, S. K., & Kim, E. C. (2008). Involvement of mitogen-activated protein kinases and nuclear factor-kappa B activation in nitric oxide-induced interleukin-8 expression in human pulp cells. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics, 105(5), 654-60. https://doi.org/10.1016/j.tripleo.2007.11.011
Min KS, et al. Involvement of Mitogen-activated Protein Kinases and Nuclear Factor-kappa B Activation in Nitric Oxide-induced Interleukin-8 Expression in Human Pulp Cells. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008;105(5):654-60. PubMed PMID: 18442745.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Involvement of mitogen-activated protein kinases and nuclear factor-kappa B activation in nitric oxide-induced interleukin-8 expression in human pulp cells. AU - Min,Kyung-San, AU - Kim,Hyun-Il, AU - Chang,Hoon-Sang, AU - Kim,Hyung-Ryong, AU - Pae,Hyun-Ock, AU - Chung,Hun-Taeg, AU - Hong,Seung-Heon, AU - Shin,Hong-In, AU - Hong,Chan-Ui, AU - Lee,Suk-Keun, AU - Kim,Eun-Cheol, PY - 2007/04/13/received PY - 2007/07/23/revised PY - 2007/11/13/accepted PY - 2008/4/30/pubmed PY - 2008/5/14/medline PY - 2008/4/30/entrez SP - 654 EP - 60 JF - Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics JO - Oral Surg Oral Med Oral Pathol Oral Radiol Endod VL - 105 IS - 5 N2 - OBJECTIVE: This study examined the effect of nitric oxide (NO) on interleukin-8 (IL-8) production and the involvement of mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-kappaB) signaling pathways in primary cultured human pulp cells. STUDY DESIGN: IL-8 production was measured using enzyme-linked immunosorbent assay (ELISA) and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. MAPK activation and IkappaB degradation and phosphorylation were determined by western blotting. RESULTS: Sodium nitroprusside (SNP), an NO donor, has increased IL-8 secretion and mRNA expression in a dose- and time-dependent manner. SNP induced the phosphorylation of p38 MAPK and extracellular-regulated kinase (ERK), degradation and phosphorylation of IkappaB, and activation of NF-kappaB. Furthermore, inhibition of the ERK, p38, and NF-kappaB pathways blocked SNP-induced IL-8 secretion. CONCLUSION: Human pulp cells showed NO-induced IL-8 expression via the MAPK and NF-kappaB pathways, which may play an important role in the inflammatory responses of pulp and periapical lesions. SN - 1528-395X UR - https://www.unboundmedicine.com/medline/citation/18442745/Involvement_of_mitogen_activated_protein_kinases_and_nuclear_factor_kappa_B_activation_in_nitric_oxide_induced_interleukin_8_expression_in_human_pulp_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1079-2104(07)00854-2 DB - PRIME DP - Unbound Medicine ER -