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Differences in global gene expression in peripheral blood mononuclear cells indicate a significant role of the innate responses in progression of dengue fever but not dengue hemorrhagic fever.
J Infect Dis. 2008 May 15; 197(10):1459-67.JI

Abstract

BACKGROUND

Dengue virus infection causes an array of symptoms ranging from dengue fever (DF) to dengue hemorrhagic fever (DHF). The pathophysiological processes behind these 2 clinical manifestations are unclear.

METHOD

In the present study, genomewide transcriptomes of peripheral blood mononuclear cells (PBMCs) collected from children with acute-phase DF (i.e., DF PBMCs) or acute-phase DHF (i.e., DHF PBMCs) were compared using microarray analysis. Results of genome screening were validated at the genomic and proteomics levels.

RESULTS

DHF had stronger influences on the gene expression profile than did DF. Of the affected genes, metabolic gene expression was influenced the most. For the immune response category, 17 genes were more strongly up-regulated in DF PBMCs than in DHF PBMCs. Eight of the these 17 genes were categorized as belonging to the interferon (IFN) system. The up-regulation of IFN-related genes was accompanied by strong expression of CD59, a complement inhibitor. DHF PBMCs expressed genes involved in T and B cell activation, cytokine production, complement activation, and T cell apoptosis more strongly than did DF PBMCs.

CONCLUSION

We hypothesize that, during DF, genes in the IFN system and complement inhibitor play a role in lowering virus production and reducing tissue damage. In patients with DHF, the dysfunction of immune cells, complement, and cytokines increases viral load and tissue damage.

Authors+Show Affiliations

Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand. scsul@mahidol.ac.thNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18444802

Citation

Ubol, Sukathida, et al. "Differences in Global Gene Expression in Peripheral Blood Mononuclear Cells Indicate a Significant Role of the Innate Responses in Progression of Dengue Fever but Not Dengue Hemorrhagic Fever." The Journal of Infectious Diseases, vol. 197, no. 10, 2008, pp. 1459-67.
Ubol S, Masrinoul P, Chaijaruwanich J, et al. Differences in global gene expression in peripheral blood mononuclear cells indicate a significant role of the innate responses in progression of dengue fever but not dengue hemorrhagic fever. J Infect Dis. 2008;197(10):1459-67.
Ubol, S., Masrinoul, P., Chaijaruwanich, J., Kalayanarooj, S., Charoensirisuthikul, T., & Kasisith, J. (2008). Differences in global gene expression in peripheral blood mononuclear cells indicate a significant role of the innate responses in progression of dengue fever but not dengue hemorrhagic fever. The Journal of Infectious Diseases, 197(10), 1459-67. https://doi.org/10.1086/587699
Ubol S, et al. Differences in Global Gene Expression in Peripheral Blood Mononuclear Cells Indicate a Significant Role of the Innate Responses in Progression of Dengue Fever but Not Dengue Hemorrhagic Fever. J Infect Dis. 2008 May 15;197(10):1459-67. PubMed PMID: 18444802.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differences in global gene expression in peripheral blood mononuclear cells indicate a significant role of the innate responses in progression of dengue fever but not dengue hemorrhagic fever. AU - Ubol,Sukathida, AU - Masrinoul,Promsin, AU - Chaijaruwanich,Jeerayut, AU - Kalayanarooj,Siripen, AU - Charoensirisuthikul,Takol, AU - Kasisith,Jitra, PY - 2008/5/1/pubmed PY - 2008/6/13/medline PY - 2008/5/1/entrez SP - 1459 EP - 67 JF - The Journal of infectious diseases JO - J Infect Dis VL - 197 IS - 10 N2 - BACKGROUND: Dengue virus infection causes an array of symptoms ranging from dengue fever (DF) to dengue hemorrhagic fever (DHF). The pathophysiological processes behind these 2 clinical manifestations are unclear. METHOD: In the present study, genomewide transcriptomes of peripheral blood mononuclear cells (PBMCs) collected from children with acute-phase DF (i.e., DF PBMCs) or acute-phase DHF (i.e., DHF PBMCs) were compared using microarray analysis. Results of genome screening were validated at the genomic and proteomics levels. RESULTS: DHF had stronger influences on the gene expression profile than did DF. Of the affected genes, metabolic gene expression was influenced the most. For the immune response category, 17 genes were more strongly up-regulated in DF PBMCs than in DHF PBMCs. Eight of the these 17 genes were categorized as belonging to the interferon (IFN) system. The up-regulation of IFN-related genes was accompanied by strong expression of CD59, a complement inhibitor. DHF PBMCs expressed genes involved in T and B cell activation, cytokine production, complement activation, and T cell apoptosis more strongly than did DF PBMCs. CONCLUSION: We hypothesize that, during DF, genes in the IFN system and complement inhibitor play a role in lowering virus production and reducing tissue damage. In patients with DHF, the dysfunction of immune cells, complement, and cytokines increases viral load and tissue damage. SN - 0022-1899 UR - https://www.unboundmedicine.com/medline/citation/18444802/Differences_in_global_gene_expression_in_peripheral_blood_mononuclear_cells_indicate_a_significant_role_of_the_innate_responses_in_progression_of_dengue_fever_but_not_dengue_hemorrhagic_fever_ L2 - https://academic.oup.com/jid/article-lookup/doi/10.1086/587699 DB - PRIME DP - Unbound Medicine ER -