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Induction of G2/M arrest and apoptosis by water extract of Strychni Semen in human gastric carcinoma AGS cells.
Phytother Res. 2008 Jun; 22(6):752-8.PR

Abstract

The seed of Strychnos nux-vomica (Loganiaceae) has been used in traditional Oriental medicine as a folk remedy for the treatment of cancer. However, the mechanism responsible for the anticancer effects of Strychni Semen is not clearly understood. The study tested whether and how the water extract of Strychni Semen (ESS) treatment would affect the growth of AGS human gastric carcinoma cells. ESS was found to inhibit the growth of AGS cells in a concentration-dependent manner. Cell cycle analysis showed G2/M phase arrest and apoptosis in AGS cells following ESS treatment. ESS-mediated G2/M arrest was found to be associated with up-regulation of cyclin A, Cdc2, tumor suppressor p53 and cyclin dependent kinase (Cdk) inhibitor p21(WAF1/CIP1), whereas the expressions of other G2/M regulatory proteins, including cyclin B1 and Cdk2, were down-regulated compared with the control. The induction of apoptotic cell death by ESS was associated with down-regulation of anti-apoptotic Bcl-2 and up-regulation of pro-apoptotic Bax expression. Further results indicate that caspase-3, caspase-8 and caspase-9 are all activated by ESS, together with cleavage of downstream caspase-3 target proteins. Taken together, the results of this study suggest the involvement of multiple signaling pathways targeted by ESS in mediating G2/M cell cycle arrest and apoptosis in AGS cells, and warrant further investigation.

Authors+Show Affiliations

Department of Pathology, Dongeui University College of Oriental Medicine, Busan 614-052, South Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18446845

Citation

Lee, Sang Man, et al. "Induction of G2/M Arrest and Apoptosis By Water Extract of Strychni Semen in Human Gastric Carcinoma AGS Cells." Phytotherapy Research : PTR, vol. 22, no. 6, 2008, pp. 752-8.
Lee SM, Kwon JI, Choi YH, et al. Induction of G2/M arrest and apoptosis by water extract of Strychni Semen in human gastric carcinoma AGS cells. Phytother Res. 2008;22(6):752-8.
Lee, S. M., Kwon, J. I., Choi, Y. H., Eom, H. S., & Chi, G. Y. (2008). Induction of G2/M arrest and apoptosis by water extract of Strychni Semen in human gastric carcinoma AGS cells. Phytotherapy Research : PTR, 22(6), 752-8. https://doi.org/10.1002/ptr.2355
Lee SM, et al. Induction of G2/M Arrest and Apoptosis By Water Extract of Strychni Semen in Human Gastric Carcinoma AGS Cells. Phytother Res. 2008;22(6):752-8. PubMed PMID: 18446845.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Induction of G2/M arrest and apoptosis by water extract of Strychni Semen in human gastric carcinoma AGS cells. AU - Lee,Sang Man, AU - Kwon,Jae Im, AU - Choi,Yung Hyun, AU - Eom,Hyun Sup, AU - Chi,Gyoo Yong, PY - 2008/5/1/pubmed PY - 2008/7/29/medline PY - 2008/5/1/entrez SP - 752 EP - 8 JF - Phytotherapy research : PTR JO - Phytother Res VL - 22 IS - 6 N2 - The seed of Strychnos nux-vomica (Loganiaceae) has been used in traditional Oriental medicine as a folk remedy for the treatment of cancer. However, the mechanism responsible for the anticancer effects of Strychni Semen is not clearly understood. The study tested whether and how the water extract of Strychni Semen (ESS) treatment would affect the growth of AGS human gastric carcinoma cells. ESS was found to inhibit the growth of AGS cells in a concentration-dependent manner. Cell cycle analysis showed G2/M phase arrest and apoptosis in AGS cells following ESS treatment. ESS-mediated G2/M arrest was found to be associated with up-regulation of cyclin A, Cdc2, tumor suppressor p53 and cyclin dependent kinase (Cdk) inhibitor p21(WAF1/CIP1), whereas the expressions of other G2/M regulatory proteins, including cyclin B1 and Cdk2, were down-regulated compared with the control. The induction of apoptotic cell death by ESS was associated with down-regulation of anti-apoptotic Bcl-2 and up-regulation of pro-apoptotic Bax expression. Further results indicate that caspase-3, caspase-8 and caspase-9 are all activated by ESS, together with cleavage of downstream caspase-3 target proteins. Taken together, the results of this study suggest the involvement of multiple signaling pathways targeted by ESS in mediating G2/M cell cycle arrest and apoptosis in AGS cells, and warrant further investigation. SN - 1099-1573 UR - https://www.unboundmedicine.com/medline/citation/18446845/Induction_of_G2/M_arrest_and_apoptosis_by_water_extract_of_Strychni_Semen_in_human_gastric_carcinoma_AGS_cells_ L2 - https://doi.org/10.1002/ptr.2355 DB - PRIME DP - Unbound Medicine ER -