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Direct electrochemistry and electrocatalytic activity of cytochrome c covalently immobilized on a boron-doped nanocrystalline diamond electrode.
Anal Chem. 2008 Jun 01; 80(11):4141-6.AC

Abstract

Cytochrome c (Cyt c) was covalently immobilized on a boron-doped nanocrystalline diamond (BDND) electrode via surface functionalization with undecylenic acid methyl ester and subsequent removal of the protecting ester groups to produce a carboxyl-terminated surface. Cyt c-modified BDND electrode exhibited a pair of quasi-reversible and well-defined redox peaks with a formal potential (E(0)) of 0.061 V (vs Ag/AgCl) in 0.1 M phosphate buffer solution (pH 7.0) and a surface-controlled process with a high electron transfer constant (ks) of 5.2 +/- 0.6 s(-1). The electrochemical properties of as-deposited and Cyt c-modified boron-doped microcrystalline diamond (BDMD) electrodes were also studied for comparison. Investigation of the electrocatalytic activity of the Cyt c-modified BDND electrode toward hydrogen peroxide (H2O2) revealed a rapid amperometric response (5 s). The linear range of response to H2O2 concentration was from 1 to 450 microM, and the detection limit was 0.7 microM at a signal-to-noise ratio of 3. The stability of the Cyt c-modified BDND electrode, in comparison with that of the BDMD and glassy carbon counterpart electrodes, was also evaluated.

Authors+Show Affiliations

Laboratory of Organic Optoelectric Functional Materials and Molecular Engineering, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100080, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18447324

Citation

Zhou, Yanli, et al. "Direct Electrochemistry and Electrocatalytic Activity of Cytochrome C Covalently Immobilized On a Boron-doped Nanocrystalline Diamond Electrode." Analytical Chemistry, vol. 80, no. 11, 2008, pp. 4141-6.
Zhou Y, Zhi J, Zou Y, et al. Direct electrochemistry and electrocatalytic activity of cytochrome c covalently immobilized on a boron-doped nanocrystalline diamond electrode. Anal Chem. 2008;80(11):4141-6.
Zhou, Y., Zhi, J., Zou, Y., Zhang, W., & Lee, S. T. (2008). Direct electrochemistry and electrocatalytic activity of cytochrome c covalently immobilized on a boron-doped nanocrystalline diamond electrode. Analytical Chemistry, 80(11), 4141-6. https://doi.org/10.1021/ac702417x
Zhou Y, et al. Direct Electrochemistry and Electrocatalytic Activity of Cytochrome C Covalently Immobilized On a Boron-doped Nanocrystalline Diamond Electrode. Anal Chem. 2008 Jun 1;80(11):4141-6. PubMed PMID: 18447324.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Direct electrochemistry and electrocatalytic activity of cytochrome c covalently immobilized on a boron-doped nanocrystalline diamond electrode. AU - Zhou,Yanli, AU - Zhi,Jinfang, AU - Zou,Yousheng, AU - Zhang,Wenjun, AU - Lee,Shuit-Tong, Y1 - 2008/04/30/ PY - 2008/5/2/pubmed PY - 2008/7/19/medline PY - 2008/5/2/entrez SP - 4141 EP - 6 JF - Analytical chemistry JO - Anal Chem VL - 80 IS - 11 N2 - Cytochrome c (Cyt c) was covalently immobilized on a boron-doped nanocrystalline diamond (BDND) electrode via surface functionalization with undecylenic acid methyl ester and subsequent removal of the protecting ester groups to produce a carboxyl-terminated surface. Cyt c-modified BDND electrode exhibited a pair of quasi-reversible and well-defined redox peaks with a formal potential (E(0)) of 0.061 V (vs Ag/AgCl) in 0.1 M phosphate buffer solution (pH 7.0) and a surface-controlled process with a high electron transfer constant (ks) of 5.2 +/- 0.6 s(-1). The electrochemical properties of as-deposited and Cyt c-modified boron-doped microcrystalline diamond (BDMD) electrodes were also studied for comparison. Investigation of the electrocatalytic activity of the Cyt c-modified BDND electrode toward hydrogen peroxide (H2O2) revealed a rapid amperometric response (5 s). The linear range of response to H2O2 concentration was from 1 to 450 microM, and the detection limit was 0.7 microM at a signal-to-noise ratio of 3. The stability of the Cyt c-modified BDND electrode, in comparison with that of the BDMD and glassy carbon counterpart electrodes, was also evaluated. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/18447324/Direct_electrochemistry_and_electrocatalytic_activity_of_cytochrome_c_covalently_immobilized_on_a_boron_doped_nanocrystalline_diamond_electrode_ L2 - https://doi.org/10.1021/ac702417x DB - PRIME DP - Unbound Medicine ER -