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Effects of arginine vasotocin and vasopressin receptor antagonists on Na+ and Cl- transport in the isolated skin of two frog species, Hyla japonica and Rana nigromaculata.
Gen Comp Endocrinol 2008; 157(1):63-9GC

Abstract

Physiological function of arginine vasotocin (AVT) and effect of receptor antagonists of vasopressin were electrophysiologically investigated on transepithelial transport of ions in the abdominal skin of Hyla japonica and Rana nigromaculata by means of the Ussing chamber system. Administrations of AVT and forskolin (adenylate cyclase activator) in the serosal side of normal Ringer's solution significantly increased transepithelial potential difference (PD) and short-circuit current (Isc) accounting for Na(+) influx, mucosal to serosal direction, across the skin of H. japonica. In contrast, AVT administrations significantly decreased PD but not Isc on the skin of R. nigromaculata in a concentration-dependent manner ranging from 10(-11) to 10(-8)M. Administration of 10(-5)M forskolin also significantly decreased PD in normal and low Na(+) Ringer's solution and in the presence of amiloride (Na(+) channel blocker) on the mucosal side of normal Ringer's solution. On the other hand, forskolin significantly increased PD and Isc in the Cl(-) free Ringer's solution. These results suggested that AVT and forskolin stimulated mainly Cl(-) influx across the skin of R. nigromaculata. In two frog species, the AVT actions on ion transports were inhibited by pretreatment of OPC-31260 (a vasopressin V(2) receptor antagonist) but not OPC-21268 (a vasopressin V(1) receptor antagonist). These results suggested that AVT activates adenylate cyclase via V(2)-like receptor and stimulates actively net Na(+) and net Cl(-) transports in the abdominal skin of H. japonica and R. nigromaculata, respectively.

Authors+Show Affiliations

Department of Life & Environmental Science, Graduate School of Science & Engineering, University of Toyama, 3190 Gofuku, Toyama 930-8555, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18448104

Citation

Yamada, Toshiki, et al. "Effects of Arginine Vasotocin and Vasopressin Receptor Antagonists On Na+ and Cl- Transport in the Isolated Skin of Two Frog Species, Hyla Japonica and Rana Nigromaculata." General and Comparative Endocrinology, vol. 157, no. 1, 2008, pp. 63-9.
Yamada T, Nishio T, Sano Y, et al. Effects of arginine vasotocin and vasopressin receptor antagonists on Na+ and Cl- transport in the isolated skin of two frog species, Hyla japonica and Rana nigromaculata. Gen Comp Endocrinol. 2008;157(1):63-9.
Yamada, T., Nishio, T., Sano, Y., Kawago, K., Matsuda, K., & Uchiyama, M. (2008). Effects of arginine vasotocin and vasopressin receptor antagonists on Na+ and Cl- transport in the isolated skin of two frog species, Hyla japonica and Rana nigromaculata. General and Comparative Endocrinology, 157(1), pp. 63-9. doi:10.1016/j.ygcen.2008.03.022.
Yamada T, et al. Effects of Arginine Vasotocin and Vasopressin Receptor Antagonists On Na+ and Cl- Transport in the Isolated Skin of Two Frog Species, Hyla Japonica and Rana Nigromaculata. Gen Comp Endocrinol. 2008 May 15;157(1):63-9. PubMed PMID: 18448104.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of arginine vasotocin and vasopressin receptor antagonists on Na+ and Cl- transport in the isolated skin of two frog species, Hyla japonica and Rana nigromaculata. AU - Yamada,Toshiki, AU - Nishio,Tomoko, AU - Sano,Yuji, AU - Kawago,Kazuki, AU - Matsuda,Kouhei, AU - Uchiyama,Minoru, Y1 - 2008/03/31/ PY - 2007/12/28/received PY - 2008/03/05/revised PY - 2008/03/26/accepted PY - 2008/5/2/pubmed PY - 2008/9/9/medline PY - 2008/5/2/entrez SP - 63 EP - 9 JF - General and comparative endocrinology JO - Gen. Comp. Endocrinol. VL - 157 IS - 1 N2 - Physiological function of arginine vasotocin (AVT) and effect of receptor antagonists of vasopressin were electrophysiologically investigated on transepithelial transport of ions in the abdominal skin of Hyla japonica and Rana nigromaculata by means of the Ussing chamber system. Administrations of AVT and forskolin (adenylate cyclase activator) in the serosal side of normal Ringer's solution significantly increased transepithelial potential difference (PD) and short-circuit current (Isc) accounting for Na(+) influx, mucosal to serosal direction, across the skin of H. japonica. In contrast, AVT administrations significantly decreased PD but not Isc on the skin of R. nigromaculata in a concentration-dependent manner ranging from 10(-11) to 10(-8)M. Administration of 10(-5)M forskolin also significantly decreased PD in normal and low Na(+) Ringer's solution and in the presence of amiloride (Na(+) channel blocker) on the mucosal side of normal Ringer's solution. On the other hand, forskolin significantly increased PD and Isc in the Cl(-) free Ringer's solution. These results suggested that AVT and forskolin stimulated mainly Cl(-) influx across the skin of R. nigromaculata. In two frog species, the AVT actions on ion transports were inhibited by pretreatment of OPC-31260 (a vasopressin V(2) receptor antagonist) but not OPC-21268 (a vasopressin V(1) receptor antagonist). These results suggested that AVT activates adenylate cyclase via V(2)-like receptor and stimulates actively net Na(+) and net Cl(-) transports in the abdominal skin of H. japonica and R. nigromaculata, respectively. SN - 1095-6840 UR - https://www.unboundmedicine.com/medline/citation/18448104/Effects_of_arginine_vasotocin_and_vasopressin_receptor_antagonists_on_Na+_and_Cl__transport_in_the_isolated_skin_of_two_frog_species_Hyla_japonica_and_Rana_nigromaculata_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0016-6480(08)00131-7 DB - PRIME DP - Unbound Medicine ER -