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Correlation of cellular immune responses with protection against culture-confirmed influenza virus in young children.
Clin Vaccine Immunol 2008; 15(7):1042-53CV

Abstract

The highly sensitive gamma interferon (IFN-gamma) enzyme-linked immunosorbent spot (ELISPOT) assay permits the investigation of the role of cell-mediated immunity (CMI) in the protection of young children against influenza. Preliminary studies of young children confirmed that the IFN-gamma ELISPOT assay was a more sensitive measure of influenza memory immune responses than serum antibody and that among seronegative children aged 6 to <36 months, an intranasal dose of 10(7) fluorescent focus units (FFU) of a live attenuated influenza virus vaccine (CAIV-T) elicited substantial CMI responses. A commercial inactivated influenza virus vaccine elicited CMI responses only in children with some previous exposure to related influenza viruses as determined by detectable antibody levels prevaccination. The role of CMI in actual protection against community-acquired, culture-confirmed clinical influenza by CAIV-T was investigated in a large randomized, double-blind, placebo-controlled dose-ranging efficacy trial with 2,172 children aged 6 to <36 months in the Philippines and Thailand. The estimated protection curve indicated that the majority of infants and young children with >or=100 spot-forming cells/10(6) peripheral blood mononuclear cells were protected against clinical influenza, establishing a possible target level of CMI for future influenza vaccine development. The ELISPOT assay for IFN-gamma is a sensitive and reproducible measure of CMI and memory immune responses and contributes to establishing requirements for the future development of vaccines against influenza, especially those used for children.

Authors+Show Affiliations

Wyeth Vaccines Research, 401 North Middletown Road, Pearl River, NY 10965, USA. forresb@wyeth.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Multicenter Study
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18448618

Citation

Forrest, Bruce D., et al. "Correlation of Cellular Immune Responses With Protection Against Culture-confirmed Influenza Virus in Young Children." Clinical and Vaccine Immunology : CVI, vol. 15, no. 7, 2008, pp. 1042-53.
Forrest BD, Pride MW, Dunning AJ, et al. Correlation of cellular immune responses with protection against culture-confirmed influenza virus in young children. Clin Vaccine Immunol. 2008;15(7):1042-53.
Forrest, B. D., Pride, M. W., Dunning, A. J., Capeding, M. R., Chotpitayasunondh, T., Tam, J. S., ... Gruber, W. C. (2008). Correlation of cellular immune responses with protection against culture-confirmed influenza virus in young children. Clinical and Vaccine Immunology : CVI, 15(7), pp. 1042-53. doi:10.1128/CVI.00397-07.
Forrest BD, et al. Correlation of Cellular Immune Responses With Protection Against Culture-confirmed Influenza Virus in Young Children. Clin Vaccine Immunol. 2008;15(7):1042-53. PubMed PMID: 18448618.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Correlation of cellular immune responses with protection against culture-confirmed influenza virus in young children. AU - Forrest,Bruce D, AU - Pride,Michael W, AU - Dunning,Andrew J, AU - Capeding,Maria Rosario Z, AU - Chotpitayasunondh,Tawee, AU - Tam,John S, AU - Rappaport,Ruth, AU - Eldridge,John H, AU - Gruber,William C, Y1 - 2008/04/30/ PY - 2008/5/2/pubmed PY - 2008/9/9/medline PY - 2008/5/2/entrez SP - 1042 EP - 53 JF - Clinical and vaccine immunology : CVI JO - Clin. Vaccine Immunol. VL - 15 IS - 7 N2 - The highly sensitive gamma interferon (IFN-gamma) enzyme-linked immunosorbent spot (ELISPOT) assay permits the investigation of the role of cell-mediated immunity (CMI) in the protection of young children against influenza. Preliminary studies of young children confirmed that the IFN-gamma ELISPOT assay was a more sensitive measure of influenza memory immune responses than serum antibody and that among seronegative children aged 6 to <36 months, an intranasal dose of 10(7) fluorescent focus units (FFU) of a live attenuated influenza virus vaccine (CAIV-T) elicited substantial CMI responses. A commercial inactivated influenza virus vaccine elicited CMI responses only in children with some previous exposure to related influenza viruses as determined by detectable antibody levels prevaccination. The role of CMI in actual protection against community-acquired, culture-confirmed clinical influenza by CAIV-T was investigated in a large randomized, double-blind, placebo-controlled dose-ranging efficacy trial with 2,172 children aged 6 to <36 months in the Philippines and Thailand. The estimated protection curve indicated that the majority of infants and young children with >or=100 spot-forming cells/10(6) peripheral blood mononuclear cells were protected against clinical influenza, establishing a possible target level of CMI for future influenza vaccine development. The ELISPOT assay for IFN-gamma is a sensitive and reproducible measure of CMI and memory immune responses and contributes to establishing requirements for the future development of vaccines against influenza, especially those used for children. SN - 1556-679X UR - https://www.unboundmedicine.com/medline/citation/18448618/Correlation_of_cellular_immune_responses_with_protection_against_culture_confirmed_influenza_virus_in_young_children_ L2 - http://cvi.asm.org/cgi/pmidlookup?view=long&amp;pmid=18448618 DB - PRIME DP - Unbound Medicine ER -