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Monitoring cytomegalovirus IE-1 and pp65-specific CD4+ and CD8+ T-cell responses after allogeneic stem cell transplantation may identify patients at risk for recurrent CMV reactivations.
Cytometry B Clin Cytom. 2008 Jul; 74(4):211-20.CB

Abstract

We studied the recovery of CMV-specific CD4+ and CD8+ T-cell immunity in 52 recipients of allogeneic stem cell transplantation (SCT). The proportions of IFN-gamma-producing CD4+ and CD8+ T cells upon in vitro activation using peptide pools representing the CMV pp65 and IE-1 proteins were assessed at multiple time points post SCT, and correlated with the occurrence of CMV reactivation. In a retrospective analysis, recurrent CMV reactivations occurred in 9 patients and were associated with low pp65-specific CD4+ T-cell and low IE-1-specific CD8(+) T-cell reactivities, whereas patients without detectable CMV reactivation (n = 30) or a single reactivation (n = 13) showed a better recovery of these immune responses. CD4+ T-cell responses to IE-1 were infrequent in most patients, whereas CD8+ T-cell responses to pp65 occurred frequently, but did not correlate with protection against (recurrent) reactivation. Prospectively, CMV-specific T-cell responses could be studied prior to 14 reactivation episodes in 8 patients. CD4+ T-cell responses to IE-1 and pp65 were positive in only 1 and 2 episodes, respectively. CD8+ T-cell responses against IE-1 were positive in 4, but against pp65 in 12 episodes, again showing that CD8+ T-cell reactivity against pp65 did not prevent CMV reactivation. Thus, monitoring of particular CMV-specific CD4+ and CD8+ T-cell responses after allogeneic SCT may identify patients at risk for recurrent CMV reactivations.

Authors+Show Affiliations

Department of Internal Oncology, Erasmus MC, Rotterdam, The Netherlands. j.w.gratama@erasmusmc.nlNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18454493

Citation

Gratama, Jan W., et al. "Monitoring Cytomegalovirus IE-1 and Pp65-specific CD4+ and CD8+ T-cell Responses After Allogeneic Stem Cell Transplantation May Identify Patients at Risk for Recurrent CMV Reactivations." Cytometry. Part B, Clinical Cytometry, vol. 74, no. 4, 2008, pp. 211-20.
Gratama JW, Brooimans RA, van der Holt B, et al. Monitoring cytomegalovirus IE-1 and pp65-specific CD4+ and CD8+ T-cell responses after allogeneic stem cell transplantation may identify patients at risk for recurrent CMV reactivations. Cytometry B Clin Cytom. 2008;74(4):211-20.
Gratama, J. W., Brooimans, R. A., van der Holt, B., Sintnicolaas, K., van Doornum, G., Niesters, H. G., Löwenberg, B., & Cornelissen, J. J. (2008). Monitoring cytomegalovirus IE-1 and pp65-specific CD4+ and CD8+ T-cell responses after allogeneic stem cell transplantation may identify patients at risk for recurrent CMV reactivations. Cytometry. Part B, Clinical Cytometry, 74(4), 211-20. https://doi.org/10.1002/cyto.b.20420
Gratama JW, et al. Monitoring Cytomegalovirus IE-1 and Pp65-specific CD4+ and CD8+ T-cell Responses After Allogeneic Stem Cell Transplantation May Identify Patients at Risk for Recurrent CMV Reactivations. Cytometry B Clin Cytom. 2008;74(4):211-20. PubMed PMID: 18454493.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Monitoring cytomegalovirus IE-1 and pp65-specific CD4+ and CD8+ T-cell responses after allogeneic stem cell transplantation may identify patients at risk for recurrent CMV reactivations. AU - Gratama,Jan W, AU - Brooimans,Rik A, AU - van der Holt,Bronno, AU - Sintnicolaas,Kees, AU - van Doornum,Gerard, AU - Niesters,Hubertus G, AU - Löwenberg,Bob, AU - Cornelissen,Jan J, PY - 2008/5/6/pubmed PY - 2008/9/11/medline PY - 2008/5/6/entrez SP - 211 EP - 20 JF - Cytometry. Part B, Clinical cytometry JO - Cytometry B Clin Cytom VL - 74 IS - 4 N2 - We studied the recovery of CMV-specific CD4+ and CD8+ T-cell immunity in 52 recipients of allogeneic stem cell transplantation (SCT). The proportions of IFN-gamma-producing CD4+ and CD8+ T cells upon in vitro activation using peptide pools representing the CMV pp65 and IE-1 proteins were assessed at multiple time points post SCT, and correlated with the occurrence of CMV reactivation. In a retrospective analysis, recurrent CMV reactivations occurred in 9 patients and were associated with low pp65-specific CD4+ T-cell and low IE-1-specific CD8(+) T-cell reactivities, whereas patients without detectable CMV reactivation (n = 30) or a single reactivation (n = 13) showed a better recovery of these immune responses. CD4+ T-cell responses to IE-1 were infrequent in most patients, whereas CD8+ T-cell responses to pp65 occurred frequently, but did not correlate with protection against (recurrent) reactivation. Prospectively, CMV-specific T-cell responses could be studied prior to 14 reactivation episodes in 8 patients. CD4+ T-cell responses to IE-1 and pp65 were positive in only 1 and 2 episodes, respectively. CD8+ T-cell responses against IE-1 were positive in 4, but against pp65 in 12 episodes, again showing that CD8+ T-cell reactivity against pp65 did not prevent CMV reactivation. Thus, monitoring of particular CMV-specific CD4+ and CD8+ T-cell responses after allogeneic SCT may identify patients at risk for recurrent CMV reactivations. SN - 1552-4957 UR - https://www.unboundmedicine.com/medline/citation/18454493/Monitoring_cytomegalovirus_IE_1_and_pp65_specific_CD4+_and_CD8+_T_cell_responses_after_allogeneic_stem_cell_transplantation_may_identify_patients_at_risk_for_recurrent_CMV_reactivations_ L2 - https://doi.org/10.1002/cyto.b.20420 DB - PRIME DP - Unbound Medicine ER -