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Effects of estrogen on temporal expressions of IL-1beta and IL-1ra in rat organotypic hippocampal slices exposed to oxygen-glucose deprivation.

Abstract

Anti-inflammatory action of estrogen is involved in neuroprotection but the effects of estrogen on IL-1beta and its endogenous antagonist (IL-1 ra) have not been clearly defined in the ischemic brain. This study was performed to evaluate whether estrogen affects the expression of IL-1beta or IL-1ra and the ratio of the two in the ischemic hippocampus. Rat organotypic hippocampal slices were treated with 17beta estradiol (E2, 1 nM) for 7 days, exposed to oxygen-glucose deprivation (OGD) for 30 min, and then reperfused for 72 h. CA1 neuronal death quantified by propidium iodide (PI) staining and expressions of IL-1beta and IL-1ra in slices measured by real-time PCR and Western blotting were examined. PI intensities in CA1 in slices treated with E2 were significantly reduced at 24 h and 72 h post-OGD, and IL-1beta mRNA expressions were reduced at 6 h and 24 h post-OGD. In addition, IL-1ra mRNA was significantly overexpressed and the ratio of IL-1beta to IL-1ra mRNA expression was reduced by E2 especially at 24 h. In terms of protein levels, E2 downregulated IL-1beta but upregulated IL-1ra and thereby decreased the IL-1beta/IL-1 ra ratio at 24h. These findings demonstrate that estrogen-induced protection is associated with a decrease in IL-1beta and an increase in IL-1ra expression in the ischemic hippocampus during early reperfusion periods, which suggests that modulation of IL-1beta/IL-1ra might be a part of anti-inflammatory effects of estrogen.

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  • Authors+Show Affiliations

    ,

    Department of Pharmacology, Ewha Medical Research Institute, School of Medicine, Ewha Womans University, 911-1 Mok6dong Yangcheon-gu, Seoul 158-710, Republic of Korea.

    , , ,

    Source

    Neuroscience letters 438:2 2008 Jun 20 pg 233-7

    MeSH

    Animals
    Cell Death
    Cell Survival
    Cytoprotection
    Down-Regulation
    Encephalitis
    Estradiol
    Estrogens
    Hippocampus
    Hypoxia-Ischemia, Brain
    Indicators and Reagents
    Interleukin 1 Receptor Antagonist Protein
    Interleukin-1beta
    Nerve Degeneration
    Neuroprotective Agents
    Organ Culture Techniques
    Propidium
    RNA, Messenger
    Rats
    Rats, Sprague-Dawley
    Time Factors
    Up-Regulation

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    18455872

    Citation

    Choi, Ji-Seung, et al. "Effects of Estrogen On Temporal Expressions of IL-1beta and IL-1ra in Rat Organotypic Hippocampal Slices Exposed to Oxygen-glucose Deprivation." Neuroscience Letters, vol. 438, no. 2, 2008, pp. 233-7.
    Choi JS, Kim SJ, Shin JA, et al. Effects of estrogen on temporal expressions of IL-1beta and IL-1ra in rat organotypic hippocampal slices exposed to oxygen-glucose deprivation. Neurosci Lett. 2008;438(2):233-7.
    Choi, J. S., Kim, S. J., Shin, J. A., Lee, K. E., & Park, E. M. (2008). Effects of estrogen on temporal expressions of IL-1beta and IL-1ra in rat organotypic hippocampal slices exposed to oxygen-glucose deprivation. Neuroscience Letters, 438(2), pp. 233-7. doi:10.1016/j.neulet.2008.04.026.
    Choi JS, et al. Effects of Estrogen On Temporal Expressions of IL-1beta and IL-1ra in Rat Organotypic Hippocampal Slices Exposed to Oxygen-glucose Deprivation. Neurosci Lett. 2008 Jun 20;438(2):233-7. PubMed PMID: 18455872.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Effects of estrogen on temporal expressions of IL-1beta and IL-1ra in rat organotypic hippocampal slices exposed to oxygen-glucose deprivation. AU - Choi,Ji-Seung, AU - Kim,Soo-Jung, AU - Shin,Jin A, AU - Lee,Kyung-Eun, AU - Park,Eun-Mi, Y1 - 2008/04/12/ PY - 2008/02/02/received PY - 2008/03/24/revised PY - 2008/04/09/accepted PY - 2008/5/6/pubmed PY - 2008/9/20/medline PY - 2008/5/6/entrez SP - 233 EP - 7 JF - Neuroscience letters JO - Neurosci. Lett. VL - 438 IS - 2 N2 - Anti-inflammatory action of estrogen is involved in neuroprotection but the effects of estrogen on IL-1beta and its endogenous antagonist (IL-1 ra) have not been clearly defined in the ischemic brain. This study was performed to evaluate whether estrogen affects the expression of IL-1beta or IL-1ra and the ratio of the two in the ischemic hippocampus. Rat organotypic hippocampal slices were treated with 17beta estradiol (E2, 1 nM) for 7 days, exposed to oxygen-glucose deprivation (OGD) for 30 min, and then reperfused for 72 h. CA1 neuronal death quantified by propidium iodide (PI) staining and expressions of IL-1beta and IL-1ra in slices measured by real-time PCR and Western blotting were examined. PI intensities in CA1 in slices treated with E2 were significantly reduced at 24 h and 72 h post-OGD, and IL-1beta mRNA expressions were reduced at 6 h and 24 h post-OGD. In addition, IL-1ra mRNA was significantly overexpressed and the ratio of IL-1beta to IL-1ra mRNA expression was reduced by E2 especially at 24 h. In terms of protein levels, E2 downregulated IL-1beta but upregulated IL-1ra and thereby decreased the IL-1beta/IL-1 ra ratio at 24h. These findings demonstrate that estrogen-induced protection is associated with a decrease in IL-1beta and an increase in IL-1ra expression in the ischemic hippocampus during early reperfusion periods, which suggests that modulation of IL-1beta/IL-1ra might be a part of anti-inflammatory effects of estrogen. SN - 0304-3940 UR - https://www.unboundmedicine.com/medline/citation/18455872/Effects_of_estrogen_on_temporal_expressions_of_IL_1beta_and_IL_1ra_in_rat_organotypic_hippocampal_slices_exposed_to_oxygen_glucose_deprivation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3940(08)00501-6 DB - PRIME DP - Unbound Medicine ER -