TY - JOUR T1 - Synthesis and evaluation of a selective molecularly imprinted polymer for the contraceptive drug levonorgestrel. AU - Khorrami,Afshin Rajabi, AU - Mehrseresht,Shima, Y1 - 2008/05/02/ PY - 2008/01/14/received PY - 2008/03/25/revised PY - 2008/04/13/accepted PY - 2008/5/6/pubmed PY - 2008/8/6/medline PY - 2008/5/6/entrez SP - 264 EP - 9 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 867 IS - 2 N2 - A molecularly imprinted polymer (MIP) has been prepared using levonorgestrel (LEV) as template. The polymer was synthesised in a non-covalent approach using methacrylic acid (MAA) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linking monomer via a free radical polymerization. An equivalent blank polymer was also synthesised in the absence of the template compound. Batch adsorption experiments were used to evaluate the binding affinity of the imprinted polymer. After packing MIP into a stainless steel column (150 mm x 4.6 mm i.d.), retention and elution of the template and related compounds were evaluated by high-performance liquid chromatography (HPLC). This LEV imprinted polymer was further applied for selective solid phase extraction (SPE) of LEV from human serum. It was confirmed that the binding ability of the prepared MIP for LEV was essentially sufficient in the presence of other compounds coexisting in serum sample. Therefore, as a selective and efficient solid phase material, LEV imprinted polymer has a high potential application in analysis of this steroidal hormone in clinical purposes. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/18456579/Synthesis_and_evaluation_of_a_selective_molecularly_imprinted_polymer_for_the_contraceptive_drug_levonorgestrel_ DB - PRIME DP - Unbound Medicine ER -