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Single-stranded structures are present within plasmids containing the Epstein-Barr virus latent origin of replication.
J Virol. 1991 Feb; 65(2):677-86.JV

Abstract

The Epstein-Barr virus (EBV) latent origin of plasmid replication (oriP) contains two essential regions, a family of repeats with 20 imperfect copies of a 30-bp sequence and a dyad symmetry element with four similar 30-bp repeats. Each of the repeats has an internal palindromic sequence and can bind EBNA 1, a protein that together with oriP constitutes the only viral element necessary for EBV maintenance and replication. Using single-strand-specific nucleases, we have probed plasmids containing oriP-derived sequences for the presence of secondary structural elements. Multiple single-stranded structures were detected within the oriP region. Of the two essential elements of oriP, the family of repeats seemed to extrude these structures at a much higher frequency than did sequences within the dyad symmetry region. Though negative supercoiling was found to stabilize the single-stranded structures, they showed significant stability even after linearization of the oriP plasmids. Two major single-stranded structures detected involved approximately 12 bp of DNA. These loci could be transiently unwound regions that form because of negative supercoiling and the high A + T content of this region of DNA, or they could be cruciform structures extruded within the palindromic sequences of oriP that may be important sites for protein-DNA interactions in the EBV oriP.

Authors+Show Affiliations

Department Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06510-8064.No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

1846191

Citation

Orlowski, R, and G Miller. "Single-stranded Structures Are Present Within Plasmids Containing the Epstein-Barr Virus Latent Origin of Replication." Journal of Virology, vol. 65, no. 2, 1991, pp. 677-86.
Orlowski R, Miller G. Single-stranded structures are present within plasmids containing the Epstein-Barr virus latent origin of replication. J Virol. 1991;65(2):677-86.
Orlowski, R., & Miller, G. (1991). Single-stranded structures are present within plasmids containing the Epstein-Barr virus latent origin of replication. Journal of Virology, 65(2), 677-86.
Orlowski R, Miller G. Single-stranded Structures Are Present Within Plasmids Containing the Epstein-Barr Virus Latent Origin of Replication. J Virol. 1991;65(2):677-86. PubMed PMID: 1846191.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Single-stranded structures are present within plasmids containing the Epstein-Barr virus latent origin of replication. AU - Orlowski,R, AU - Miller,G, PY - 1991/2/1/pubmed PY - 1991/2/1/medline PY - 1991/2/1/entrez SP - 677 EP - 86 JF - Journal of virology JO - J Virol VL - 65 IS - 2 N2 - The Epstein-Barr virus (EBV) latent origin of plasmid replication (oriP) contains two essential regions, a family of repeats with 20 imperfect copies of a 30-bp sequence and a dyad symmetry element with four similar 30-bp repeats. Each of the repeats has an internal palindromic sequence and can bind EBNA 1, a protein that together with oriP constitutes the only viral element necessary for EBV maintenance and replication. Using single-strand-specific nucleases, we have probed plasmids containing oriP-derived sequences for the presence of secondary structural elements. Multiple single-stranded structures were detected within the oriP region. Of the two essential elements of oriP, the family of repeats seemed to extrude these structures at a much higher frequency than did sequences within the dyad symmetry region. Though negative supercoiling was found to stabilize the single-stranded structures, they showed significant stability even after linearization of the oriP plasmids. Two major single-stranded structures detected involved approximately 12 bp of DNA. These loci could be transiently unwound regions that form because of negative supercoiling and the high A + T content of this region of DNA, or they could be cruciform structures extruded within the palindromic sequences of oriP that may be important sites for protein-DNA interactions in the EBV oriP. SN - 0022-538X UR - https://www.unboundmedicine.com/medline/citation/1846191/Single_stranded_structures_are_present_within_plasmids_containing_the_Epstein_Barr_virus_latent_origin_of_replication_ L2 - http://jvi.asm.org/cgi/pmidlookup?view=long&pmid=1846191 DB - PRIME DP - Unbound Medicine ER -