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Inhibition of tumor necrosis factor-alpha reduces alveolar septal cell apoptosis in passive smoking rats.
Chin Med J (Engl). 2008 Apr 05; 121(7):597-601.CM

Abstract

BACKGROUND

Recent studies have revealed that lung cell apoptosis plays an important role in pathogenesis of cigarette-induced chronic obstructive pulmonary disease (COPD). Tumor necrosis factor alpha (TNF-alpha) is one of the most important cytokines which are involved in COPD. This study aimed at investigating the influence of its inhibitor, recombinant human necrosis factor-alpha receptor II:IgG Fc fusion protein (rhTNFR:Fc) on alveolar septal cell apoptosis in passive smoking rats.

METHODS

Forty-eight rats were randomly divided into a normal control group, a passive smoking group, an rhTNFR:Fc intervention group and a sham intervention group. The passive smoking rats were treated by exposure to cigarette smoking daily for 80 days. After smoking for one month the rhTNFR:Fc intervention group was treated with rhTNFR:Fc by subcutaneous injection, the sham intervention group injected subcutaneously with a neutral preparation (normal saline 0.1 ml, manicol 0.8 ml, cane sugar 0.2 mg, Tris 0.024 mg as a control. Lung function was determined and the levels of TNF-alpha in serum and broncho-alveolar lavage fluid (BALF) were measured with enzyme-linked immunosorbnent assay (ELISA). Lung tissue sections stained by hematoxylin and eosin (HE) were observed for study of morphological alternations. Mean linear intercept (MLI) and mean alveolar numbers (MAN) were measured and the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) method was carried out to determine the percentage of positive cells and distribution of apoptotic cells.

RESULTS

Increased MLI and decreased MAN were found in the passive smoking group compared with both the normal control group and the rhTNFR:Fc intervention group (P < 0.05). Forced expiratory volume in 0.3 second (FEV 0.3)/forced vital capacity (FVC) and peak expiratory flow (PEF) were lower in the passive smoking group than that in the normal control group (P < 0.05). Compared with the sham intervention group, FEV 0.3/FVC and PEF increased in the rhTNFR:Fc intervention group (P < 0.05). The levels of TNF-alpha in serum were higher in the passive smoking group than that in the normal control group (P < 0.05) and rhTNFR:Fc intervention group (P < 0.05). Significant differences were found between the levels of TNF-alpha in the serum of the rhTNFR:Fc intervention group and sham intervention group (P < 0.05). The levels of TNF-alpha in BALF were higher in the passive smoking group than that in the normal control group (P < 0.05), but no significant differences of TNF-alpha levels in BALF were found between the passive smoking group and rhTNFR:Fc intervention group. The number of TUNEL positive cells in alveolar septa was significantly increased in the passive smoking group as compared with the normal control group and the rhTNFR:Fc intervention group (P < 0.05).

CONCLUSION

This study provides preliminary evidence that rhTNFR:Fc may interfere with TNF-alpha and reduce alveolar septal apoptosis in smoking rats.

Authors+Show Affiliations

Department of Respiratory Medicine, Second Xiangya Hospital of Central-South University, Changsha, Hunan 410011, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18466678

Citation

Zhang, Cheng, et al. "Inhibition of Tumor Necrosis Factor-alpha Reduces Alveolar Septal Cell Apoptosis in Passive Smoking Rats." Chinese Medical Journal, vol. 121, no. 7, 2008, pp. 597-601.
Zhang C, Cai S, Chen P, et al. Inhibition of tumor necrosis factor-alpha reduces alveolar septal cell apoptosis in passive smoking rats. Chin Med J (Engl). 2008;121(7):597-601.
Zhang, C., Cai, S., Chen, P., Chen, J. B., Wu, J., Wu, S. J., & Zhou, R. (2008). Inhibition of tumor necrosis factor-alpha reduces alveolar septal cell apoptosis in passive smoking rats. Chinese Medical Journal, 121(7), 597-601.
Zhang C, et al. Inhibition of Tumor Necrosis Factor-alpha Reduces Alveolar Septal Cell Apoptosis in Passive Smoking Rats. Chin Med J (Engl). 2008 Apr 5;121(7):597-601. PubMed PMID: 18466678.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of tumor necrosis factor-alpha reduces alveolar septal cell apoptosis in passive smoking rats. AU - Zhang,Cheng, AU - Cai,Shan, AU - Chen,Ping, AU - Chen,Jian-bo, AU - Wu,Jie, AU - Wu,Shang-jie, AU - Zhou,Rui, PY - 2008/5/10/pubmed PY - 2008/7/9/medline PY - 2008/5/10/entrez SP - 597 EP - 601 JF - Chinese medical journal JO - Chin Med J (Engl) VL - 121 IS - 7 N2 - BACKGROUND: Recent studies have revealed that lung cell apoptosis plays an important role in pathogenesis of cigarette-induced chronic obstructive pulmonary disease (COPD). Tumor necrosis factor alpha (TNF-alpha) is one of the most important cytokines which are involved in COPD. This study aimed at investigating the influence of its inhibitor, recombinant human necrosis factor-alpha receptor II:IgG Fc fusion protein (rhTNFR:Fc) on alveolar septal cell apoptosis in passive smoking rats. METHODS: Forty-eight rats were randomly divided into a normal control group, a passive smoking group, an rhTNFR:Fc intervention group and a sham intervention group. The passive smoking rats were treated by exposure to cigarette smoking daily for 80 days. After smoking for one month the rhTNFR:Fc intervention group was treated with rhTNFR:Fc by subcutaneous injection, the sham intervention group injected subcutaneously with a neutral preparation (normal saline 0.1 ml, manicol 0.8 ml, cane sugar 0.2 mg, Tris 0.024 mg as a control. Lung function was determined and the levels of TNF-alpha in serum and broncho-alveolar lavage fluid (BALF) were measured with enzyme-linked immunosorbnent assay (ELISA). Lung tissue sections stained by hematoxylin and eosin (HE) were observed for study of morphological alternations. Mean linear intercept (MLI) and mean alveolar numbers (MAN) were measured and the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) method was carried out to determine the percentage of positive cells and distribution of apoptotic cells. RESULTS: Increased MLI and decreased MAN were found in the passive smoking group compared with both the normal control group and the rhTNFR:Fc intervention group (P < 0.05). Forced expiratory volume in 0.3 second (FEV 0.3)/forced vital capacity (FVC) and peak expiratory flow (PEF) were lower in the passive smoking group than that in the normal control group (P < 0.05). Compared with the sham intervention group, FEV 0.3/FVC and PEF increased in the rhTNFR:Fc intervention group (P < 0.05). The levels of TNF-alpha in serum were higher in the passive smoking group than that in the normal control group (P < 0.05) and rhTNFR:Fc intervention group (P < 0.05). Significant differences were found between the levels of TNF-alpha in the serum of the rhTNFR:Fc intervention group and sham intervention group (P < 0.05). The levels of TNF-alpha in BALF were higher in the passive smoking group than that in the normal control group (P < 0.05), but no significant differences of TNF-alpha levels in BALF were found between the passive smoking group and rhTNFR:Fc intervention group. The number of TUNEL positive cells in alveolar septa was significantly increased in the passive smoking group as compared with the normal control group and the rhTNFR:Fc intervention group (P < 0.05). CONCLUSION: This study provides preliminary evidence that rhTNFR:Fc may interfere with TNF-alpha and reduce alveolar septal apoptosis in smoking rats. SN - 0366-6999 UR - https://www.unboundmedicine.com/medline/citation/18466678/Inhibition_of_tumor_necrosis_factor_alpha_reduces_alveolar_septal_cell_apoptosis_in_passive_smoking_rats_ L2 - https://Insights.ovid.com/pubmed?pmid=18466678 DB - PRIME DP - Unbound Medicine ER -