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Highly sensitive detection of pharmaceutical compounds in biological fluids using capillary electrophoresis coupled with laser-induced native fluorescence.
J Chromatogr A. 2008 Sep 19; 1204(2):183-90.JC

Abstract

Due to its selectivity and sensitivity, capillary electrophoresis coupled to laser-induced fluorescence has evolved as a useful analytical tool for determining drugs in biological samples. This paper describes a simple, sensitive, efficient, and rapid method for analyzing propranolol without derivatization in plasma by CE-LIF using a diode solid-state laser at 266nm. An experimental design methodology was assessed for the investigation of electrophoretic parameters and a Box-Behnken design was selected to optimize both sensitivity and efficiency. The CE-LIF method was linear over the concentration range of 50-850ngmL(-1) and successfully applied to a real forensic sample.

Authors+Show Affiliations

Laboratory of Pharmaceutical Analytical Chemistry, School of Pharmaceutical Sciences - EPGL, University of Geneva, University of Lausanne, Bd d'Yvoy 20, 1211 Geneva 4, Switzerland.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18468608

Citation

Schappler, Julie, et al. "Highly Sensitive Detection of Pharmaceutical Compounds in Biological Fluids Using Capillary Electrophoresis Coupled With Laser-induced Native Fluorescence." Journal of Chromatography. A, vol. 1204, no. 2, 2008, pp. 183-90.
Schappler J, Staub A, Veuthey JL, et al. Highly sensitive detection of pharmaceutical compounds in biological fluids using capillary electrophoresis coupled with laser-induced native fluorescence. J Chromatogr A. 2008;1204(2):183-90.
Schappler, J., Staub, A., Veuthey, J. L., & Rudaz, S. (2008). Highly sensitive detection of pharmaceutical compounds in biological fluids using capillary electrophoresis coupled with laser-induced native fluorescence. Journal of Chromatography. A, 1204(2), 183-90. https://doi.org/10.1016/j.chroma.2008.04.044
Schappler J, et al. Highly Sensitive Detection of Pharmaceutical Compounds in Biological Fluids Using Capillary Electrophoresis Coupled With Laser-induced Native Fluorescence. J Chromatogr A. 2008 Sep 19;1204(2):183-90. PubMed PMID: 18468608.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Highly sensitive detection of pharmaceutical compounds in biological fluids using capillary electrophoresis coupled with laser-induced native fluorescence. AU - Schappler,Julie, AU - Staub,Aline, AU - Veuthey,Jean-Luc, AU - Rudaz,Serge, Y1 - 2008/04/23/ PY - 2008/01/21/received PY - 2008/04/18/revised PY - 2008/04/21/accepted PY - 2008/5/13/pubmed PY - 2008/10/24/medline PY - 2008/5/13/entrez SP - 183 EP - 90 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1204 IS - 2 N2 - Due to its selectivity and sensitivity, capillary electrophoresis coupled to laser-induced fluorescence has evolved as a useful analytical tool for determining drugs in biological samples. This paper describes a simple, sensitive, efficient, and rapid method for analyzing propranolol without derivatization in plasma by CE-LIF using a diode solid-state laser at 266nm. An experimental design methodology was assessed for the investigation of electrophoretic parameters and a Box-Behnken design was selected to optimize both sensitivity and efficiency. The CE-LIF method was linear over the concentration range of 50-850ngmL(-1) and successfully applied to a real forensic sample. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/18468608/Highly_sensitive_detection_of_pharmaceutical_compounds_in_biological_fluids_using_capillary_electrophoresis_coupled_with_laser_induced_native_fluorescence_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(08)00744-9 DB - PRIME DP - Unbound Medicine ER -