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A new protein A assay based on Raman reporter labeled immunogold nanoparticles.
Biosens Bioelectron. 2008 Oct 15; 24(2):178-83.BB

Abstract

A unique, sensitive, highly specific, and photobleaching-resistant immunoassay system utilizing gold nanoparticles and surface-enhanced Raman scattering (SERS) is described. This new system, featuring a capability of bifunctional analysis, is manufactured by chemisorption of antibody immunoglobulin G (IgG) on gold nanoparticles (AuNP), followed by coupling the Raman-active reporter molecule, 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) to the surface of IgG-AuNP. The adsorbed DTNB molecules exhibit strong Raman signals via both electromagnetic and chemical enhancement. The narrow spectral widths and high photostability assure the system to be an excellent detection label. This SERS-based immunoassay is applied to the detection of protein A, which is a specific surface antigen of Staphylococcus aureus. A working curve is obtained by plotting the intensity of the SERS signal of symmetric NO(2) stretching of DTNB at 1,333 cm(-1) versus the concentration of the analyte (antigen). A dynamic range of two to three orders of magnitude and a detection limit of 1 pg/mL of protein A are achieved.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Lin CC
Institute of Biomedical Engineering, National Cheng Kung University, Tainan 701, Taiwan, ROC.
Yang YM
No affiliation info available
Chen YF
No affiliation info available
Yang TS
No affiliation info available
Chang HC
No affiliation info available

MeSH

Biosensing TechniquesGoldImmunoassayNanoparticlesNanotechnologySpectrum Analysis, RamanStaining and LabelingStaphylococcal Protein A

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18468881

Citation

Lin, Chi-Chang, et al. "A New Protein a Assay Based On Raman Reporter Labeled Immunogold Nanoparticles." Biosensors & Bioelectronics, vol. 24, no. 2, 2008, pp. 178-83.
Lin CC, Yang YM, Chen YF, et al. A new protein A assay based on Raman reporter labeled immunogold nanoparticles. Biosens Bioelectron. 2008;24(2):178-83.
Lin, C. C., Yang, Y. M., Chen, Y. F., Yang, T. S., & Chang, H. C. (2008). A new protein A assay based on Raman reporter labeled immunogold nanoparticles. Biosensors & Bioelectronics, 24(2), 178-83. https://doi.org/10.1016/j.bios.2008.03.035
Lin CC, et al. A New Protein a Assay Based On Raman Reporter Labeled Immunogold Nanoparticles. Biosens Bioelectron. 2008 Oct 15;24(2):178-83. PubMed PMID: 18468881.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A new protein A assay based on Raman reporter labeled immunogold nanoparticles. AU - Lin,Chi-Chang, AU - Yang,Ying-Mei, AU - Chen,Yan-Fu, AU - Yang,Tzyy-Schiuan, AU - Chang,Hsien-Chang, Y1 - 2008/04/04/ PY - 2008/01/02/received PY - 2008/03/18/revised PY - 2008/03/25/accepted PY - 2008/5/13/pubmed PY - 2008/11/19/medline PY - 2008/5/13/entrez SP - 178 EP - 83 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 24 IS - 2 N2 - A unique, sensitive, highly specific, and photobleaching-resistant immunoassay system utilizing gold nanoparticles and surface-enhanced Raman scattering (SERS) is described. This new system, featuring a capability of bifunctional analysis, is manufactured by chemisorption of antibody immunoglobulin G (IgG) on gold nanoparticles (AuNP), followed by coupling the Raman-active reporter molecule, 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) to the surface of IgG-AuNP. The adsorbed DTNB molecules exhibit strong Raman signals via both electromagnetic and chemical enhancement. The narrow spectral widths and high photostability assure the system to be an excellent detection label. This SERS-based immunoassay is applied to the detection of protein A, which is a specific surface antigen of Staphylococcus aureus. A working curve is obtained by plotting the intensity of the SERS signal of symmetric NO(2) stretching of DTNB at 1,333 cm(-1) versus the concentration of the analyte (antigen). A dynamic range of two to three orders of magnitude and a detection limit of 1 pg/mL of protein A are achieved. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/18468881/A_new_protein_A_assay_based_on_Raman_reporter_labeled_immunogold_nanoparticles_ DB - PRIME DP - Unbound Medicine ER -