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Nanobioengineered electrospun composite nanofibers and osteoblasts for bone regeneration.
Artif Organs 2008; 32(5):388-97AO

Abstract

Bone defects represent a medical and socioeconomic challenge. Engineering bioartificial bone tissues may help to solve problems related to donor site morbidity and size limitations. Nanofibrous scaffolds were electrospun into a blend of synthetic biodegradable polycaprolactone (PCL) with hydroxyapatite (HA) and natural polymer gelatin (Gel) at a ratio of 1:1:2 (PCL/HA/Gel) compared to PCL (9%), PCL/HA (1:1), and PCL/Gel (1:2) nanofibers. These fiber diameters were around 411 +/- 158 to 856 +/- 157 nm, and the pore size and porosity around 5-35 microm and 76-93%, respectively. The interconnecting porous structure of the nanofibrous scaffolds provides large surface area for cell attachment and sufficient space for nutrient transportation. The tensile property of composite nanofibrous scaffold (PCL/HA/Gel) was highly flexible and allows penetrating osteoblasts inside the scaffolds for bone tissue regeneration. Fourier transform infrared analysis showed that the composite nanofiber contains an amino group, a phosphate group, and carboxyl groups for inducing proliferation and mineralization of osteoblasts for in vitro bone formation. The cell proliferation (88%), alkaline phosphatase activity (77%), and mineralization (66%) of osteoblasts were significantly (P < 0.001) increased in composite nanofibrous scaffold compared to PCL nanofibrous scaffolds. Field emission scanning electron microscopic images showed that the composite nanofibers supported the proliferation and mineralization of osteoblast cells. These results show that the fabrication of electrospun PCL/HA/Gel composite nanofibrous scaffolds has potential for the proliferation and mineralization of osteoblasts for bone regeneration.

Authors+Show Affiliations

Nanoscience and Nanotechnology Initiative, Division of Bioengineering, National University of Singapore, Singapore. engjrv@nus.edu.sgNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18471168

Citation

Venugopal, Jayarama Reddy, et al. "Nanobioengineered Electrospun Composite Nanofibers and Osteoblasts for Bone Regeneration." Artificial Organs, vol. 32, no. 5, 2008, pp. 388-97.
Venugopal JR, Low S, Choon AT, et al. Nanobioengineered electrospun composite nanofibers and osteoblasts for bone regeneration. Artif Organs. 2008;32(5):388-97.
Venugopal, J. R., Low, S., Choon, A. T., Kumar, A. B., & Ramakrishna, S. (2008). Nanobioengineered electrospun composite nanofibers and osteoblasts for bone regeneration. Artificial Organs, 32(5), pp. 388-97. doi:10.1111/j.1525-1594.2008.00557.x.
Venugopal JR, et al. Nanobioengineered Electrospun Composite Nanofibers and Osteoblasts for Bone Regeneration. Artif Organs. 2008;32(5):388-97. PubMed PMID: 18471168.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nanobioengineered electrospun composite nanofibers and osteoblasts for bone regeneration. AU - Venugopal,Jayarama Reddy, AU - Low,Sharon, AU - Choon,Aw Tar, AU - Kumar,A Bharath, AU - Ramakrishna,Seeram, PY - 2008/5/13/pubmed PY - 2008/6/25/medline PY - 2008/5/13/entrez SP - 388 EP - 97 JF - Artificial organs JO - Artif Organs VL - 32 IS - 5 N2 - Bone defects represent a medical and socioeconomic challenge. Engineering bioartificial bone tissues may help to solve problems related to donor site morbidity and size limitations. Nanofibrous scaffolds were electrospun into a blend of synthetic biodegradable polycaprolactone (PCL) with hydroxyapatite (HA) and natural polymer gelatin (Gel) at a ratio of 1:1:2 (PCL/HA/Gel) compared to PCL (9%), PCL/HA (1:1), and PCL/Gel (1:2) nanofibers. These fiber diameters were around 411 +/- 158 to 856 +/- 157 nm, and the pore size and porosity around 5-35 microm and 76-93%, respectively. The interconnecting porous structure of the nanofibrous scaffolds provides large surface area for cell attachment and sufficient space for nutrient transportation. The tensile property of composite nanofibrous scaffold (PCL/HA/Gel) was highly flexible and allows penetrating osteoblasts inside the scaffolds for bone tissue regeneration. Fourier transform infrared analysis showed that the composite nanofiber contains an amino group, a phosphate group, and carboxyl groups for inducing proliferation and mineralization of osteoblasts for in vitro bone formation. The cell proliferation (88%), alkaline phosphatase activity (77%), and mineralization (66%) of osteoblasts were significantly (P < 0.001) increased in composite nanofibrous scaffold compared to PCL nanofibrous scaffolds. Field emission scanning electron microscopic images showed that the composite nanofibers supported the proliferation and mineralization of osteoblast cells. These results show that the fabrication of electrospun PCL/HA/Gel composite nanofibrous scaffolds has potential for the proliferation and mineralization of osteoblasts for bone regeneration. SN - 1525-1594 UR - https://www.unboundmedicine.com/medline/citation/18471168/Nanobioengineered_electrospun_composite_nanofibers_and_osteoblasts_for_bone_regeneration_ L2 - https://doi.org/10.1111/j.1525-1594.2008.00557.x DB - PRIME DP - Unbound Medicine ER -