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[Isoflurane pretreatment reduced liver injury induced by ischemia/reperfusion combined with lipopolysaccharide in rats].
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2008 May; 20(5):271-4.ZW

Abstract

OBJECTIVE

To investigate the effect of lipopolysaccharide (LPS) on ischemia/reperfusion (I/R) injury of liver and the protective effect of isoflurane (ISO) pretreatment on such injury in rat.

METHODS

Thirty-two male Sprague-Dawley(SD) rats were randomly assigned to 4 groups: Sham group, only receive anesthesia and laparotomy; I/R group; I/R+LPS group, with 1 hour of hepatic ischemia and 4 hours of reperfusion, and LPS was given at the beginning of reperfusion; ISO group, received ISO pretreatment for 0.5 hour, then 1 hour of hepatic ischemia followed by 4 hours of reperfusion and LPS given at the beginning of reperfusion. The pathological changes in the liver tissue were assessed. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), the myeloperoxidase (MPO) activity in liver tissue, hepatic and serum tumor necrosis factor-alpha (TNF-alpha) were determined.

RESULTS

Compared with Sham group, ALT, AST, TNF-alpha in serum and MPO activity in liver tissue, hepatic and serum TNF-alpha were increased significantly in all injury groups (all P<0.01). Compared with ISO group alone, hepatic I/R combined with LPS resulted in severer liver injury, with the levels of ALT, AST in serum, MPO activity in the liver tissue, and hepatic and serum TNF-alpha level were all increased (all P<0.05). Compared with I/R+LPS group, both the liver injury and inflammatory reaction were significantly reduced in I/R group (P<0.05 or P<0.01).

CONCLUSION

LPS injection during reperfusion results in severer liver injury and inflammatory reaction after hepatic I/R in rats. ISO pretreatment for 30 minutes might reduce the inflammatory reaction and live injury induced by hepatic I/R combined with LPS.

Authors+Show Affiliations

Department of Anesthesiology, Eastern Hepatobiliary Hospital, Second Military Medical University, Shanghai 200438, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

18471356

Citation

Lv, Xin, et al. "[Isoflurane Pretreatment Reduced Liver Injury Induced By Ischemia/reperfusion Combined With Lipopolysaccharide in Rats]." Zhongguo Wei Zhong Bing Ji Jiu Yi Xue = Chinese Critical Care Medicine = Zhongguo Weizhongbing Jijiuyixue, vol. 20, no. 5, 2008, pp. 271-4.
Lv X, Li Q, Huang SD, et al. [Isoflurane pretreatment reduced liver injury induced by ischemia/reperfusion combined with lipopolysaccharide in rats]. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2008;20(5):271-4.
Lv, X., Li, Q., Huang, S. D., Wu, F. X., Yang, L. Q., & Yu, W. F. (2008). [Isoflurane pretreatment reduced liver injury induced by ischemia/reperfusion combined with lipopolysaccharide in rats]. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue = Chinese Critical Care Medicine = Zhongguo Weizhongbing Jijiuyixue, 20(5), 271-4.
Lv X, et al. [Isoflurane Pretreatment Reduced Liver Injury Induced By Ischemia/reperfusion Combined With Lipopolysaccharide in Rats]. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2008;20(5):271-4. PubMed PMID: 18471356.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Isoflurane pretreatment reduced liver injury induced by ischemia/reperfusion combined with lipopolysaccharide in rats]. AU - Lv,Xin, AU - Li,Quan, AU - Huang,Sheng-Dong, AU - Wu,Fei-Xiang, AU - Yang,Li-Qun, AU - Yu,Wei-Feng, PY - 2008/5/13/pubmed PY - 2010/2/5/medline PY - 2008/5/13/entrez SP - 271 EP - 4 JF - Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue JO - Zhongguo Wei Zhong Bing Ji Jiu Yi Xue VL - 20 IS - 5 N2 - OBJECTIVE: To investigate the effect of lipopolysaccharide (LPS) on ischemia/reperfusion (I/R) injury of liver and the protective effect of isoflurane (ISO) pretreatment on such injury in rat. METHODS: Thirty-two male Sprague-Dawley(SD) rats were randomly assigned to 4 groups: Sham group, only receive anesthesia and laparotomy; I/R group; I/R+LPS group, with 1 hour of hepatic ischemia and 4 hours of reperfusion, and LPS was given at the beginning of reperfusion; ISO group, received ISO pretreatment for 0.5 hour, then 1 hour of hepatic ischemia followed by 4 hours of reperfusion and LPS given at the beginning of reperfusion. The pathological changes in the liver tissue were assessed. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), the myeloperoxidase (MPO) activity in liver tissue, hepatic and serum tumor necrosis factor-alpha (TNF-alpha) were determined. RESULTS: Compared with Sham group, ALT, AST, TNF-alpha in serum and MPO activity in liver tissue, hepatic and serum TNF-alpha were increased significantly in all injury groups (all P<0.01). Compared with ISO group alone, hepatic I/R combined with LPS resulted in severer liver injury, with the levels of ALT, AST in serum, MPO activity in the liver tissue, and hepatic and serum TNF-alpha level were all increased (all P<0.05). Compared with I/R+LPS group, both the liver injury and inflammatory reaction were significantly reduced in I/R group (P<0.05 or P<0.01). CONCLUSION: LPS injection during reperfusion results in severer liver injury and inflammatory reaction after hepatic I/R in rats. ISO pretreatment for 30 minutes might reduce the inflammatory reaction and live injury induced by hepatic I/R combined with LPS. SN - 1003-0603 UR - https://www.unboundmedicine.com/medline/citation/18471356/[Isoflurane_pretreatment_reduced_liver_injury_induced_by_ischemia/reperfusion_combined_with_lipopolysaccharide_in_rats]_ DB - PRIME DP - Unbound Medicine ER -