Tags

Type your tag names separated by a space and hit enter

[Urine protein from patients with lupus nephritis stimulates transdifferentiation and high expression of collagen in renal tubular cells].
Zhonghua Yi Xue Za Zhi. 2007 Dec 25; 87(48):3429-32.ZY

Abstract

OBJECTIVE

To investigate the effects of urine protein on the renal tubular-interstitial fibrosis in the patients with lupus nephritis (LN).

METHODS

Protein was isolated and purified from the urine of six patients with primary LN, 1 male and 5 females, aged 27.4, and incubated with renal tubular cells of the line HK-2 for 0, 1, 2, 12, 24, or 48 h respectively. The mRNA expressions of transforming growth factor beta1(TGF-beta1), collagen I (COL I), and alpha-smooth muscle actin (alpha-SMA) in the HK-2 cells were detected by RT-PCR, and the. protein expressions of TGF-beta1, COL I, and alpha-SMA were detected with Western blotting and indirect immunofluorescence.

RESULTS

The urine protein from the LN patients dose-and time-dependently increased the mRNA and protein expressions of TGF-beta1, COL I, and alpha-SMA in the HK-2 cells. The TGF-beta1 mRNA level 48 h after incubation was 0.39 +/- 0.03, significantly higher than that at the beginning of incubation (0.27 +/- 0.02, P < 0.01), and the TGF-beta1 protein level 48 h after incubation was 0.37 +/- 0.03, 1.7 times that at the beginning of incubation (0.27 +/- 0.04, P < 0.01). The COL I mRNA level 48 h after incubation was 0.38 +/- 0.02, significantly higher than the baseline level (0.22 +/- 0.03, P < 0.01); and the COL I protein level 48 h after incubation was 0.44 +/- 0.03, significantly higher than the baseline level (0.19 +/- 0.02, P < 0.01). The alpha-SMA mRNA level 48 h after incubation was 0.66 +/- 0.04, significantly higher than the baseline level (0.44 +/- 0.03, P < 0.01), and the alpha-SMA protein level 48 h after incubation was 0.43 +/- 0.02, significantly higher than the baseline level (0.24 +/- 0.03, P < 0.01).

CONCLUSION

Urine protein may play an important role in the renal tubular-interstitial fibrosis by inducing the production of extracellular matrix and phenotype change in HK-2 cells.

Authors+Show Affiliations

Department of Nephrology, Huizhou Municipal Central Hospital, China, Huizhou 516001, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

18476545

Citation

Liu, Guan-xian, et al. "[Urine Protein From Patients With Lupus Nephritis Stimulates Transdifferentiation and High Expression of Collagen in Renal Tubular Cells]." Zhonghua Yi Xue Za Zhi, vol. 87, no. 48, 2007, pp. 3429-32.
Liu GX, Deng AG, Yu XQ, et al. [Urine protein from patients with lupus nephritis stimulates transdifferentiation and high expression of collagen in renal tubular cells]. Zhonghua Yi Xue Za Zhi. 2007;87(48):3429-32.
Liu, G. X., Deng, A. G., Yu, X. Q., Duan, W. J., Wen, Q., Luo, N., & Li, X. Y. (2007). [Urine protein from patients with lupus nephritis stimulates transdifferentiation and high expression of collagen in renal tubular cells]. Zhonghua Yi Xue Za Zhi, 87(48), 3429-32.
Liu GX, et al. [Urine Protein From Patients With Lupus Nephritis Stimulates Transdifferentiation and High Expression of Collagen in Renal Tubular Cells]. Zhonghua Yi Xue Za Zhi. 2007 Dec 25;87(48):3429-32. PubMed PMID: 18476545.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Urine protein from patients with lupus nephritis stimulates transdifferentiation and high expression of collagen in renal tubular cells]. AU - Liu,Guan-xian, AU - Deng,An-guo, AU - Yu,Xue-qing, AU - Duan,Wen-juan, AU - Wen,Qiong, AU - Luo,Ning, AU - Li,Xiao-yan, PY - 2008/5/15/pubmed PY - 2008/8/30/medline PY - 2008/5/15/entrez SP - 3429 EP - 32 JF - Zhonghua yi xue za zhi JO - Zhonghua Yi Xue Za Zhi VL - 87 IS - 48 N2 - OBJECTIVE: To investigate the effects of urine protein on the renal tubular-interstitial fibrosis in the patients with lupus nephritis (LN). METHODS: Protein was isolated and purified from the urine of six patients with primary LN, 1 male and 5 females, aged 27.4, and incubated with renal tubular cells of the line HK-2 for 0, 1, 2, 12, 24, or 48 h respectively. The mRNA expressions of transforming growth factor beta1(TGF-beta1), collagen I (COL I), and alpha-smooth muscle actin (alpha-SMA) in the HK-2 cells were detected by RT-PCR, and the. protein expressions of TGF-beta1, COL I, and alpha-SMA were detected with Western blotting and indirect immunofluorescence. RESULTS: The urine protein from the LN patients dose-and time-dependently increased the mRNA and protein expressions of TGF-beta1, COL I, and alpha-SMA in the HK-2 cells. The TGF-beta1 mRNA level 48 h after incubation was 0.39 +/- 0.03, significantly higher than that at the beginning of incubation (0.27 +/- 0.02, P < 0.01), and the TGF-beta1 protein level 48 h after incubation was 0.37 +/- 0.03, 1.7 times that at the beginning of incubation (0.27 +/- 0.04, P < 0.01). The COL I mRNA level 48 h after incubation was 0.38 +/- 0.02, significantly higher than the baseline level (0.22 +/- 0.03, P < 0.01); and the COL I protein level 48 h after incubation was 0.44 +/- 0.03, significantly higher than the baseline level (0.19 +/- 0.02, P < 0.01). The alpha-SMA mRNA level 48 h after incubation was 0.66 +/- 0.04, significantly higher than the baseline level (0.44 +/- 0.03, P < 0.01), and the alpha-SMA protein level 48 h after incubation was 0.43 +/- 0.02, significantly higher than the baseline level (0.24 +/- 0.03, P < 0.01). CONCLUSION: Urine protein may play an important role in the renal tubular-interstitial fibrosis by inducing the production of extracellular matrix and phenotype change in HK-2 cells. SN - 0376-2491 UR - https://www.unboundmedicine.com/medline/citation/18476545/[Urine_protein_from_patients_with_lupus_nephritis_stimulates_transdifferentiation_and_high_expression_of_collagen_in_renal_tubular_cells]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&amp;issn=0376-2491&amp;year=2007&amp;vol=87&amp;issue=48&amp;fpage=3429 DB - PRIME DP - Unbound Medicine ER -