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[Effects of vascular endothelial growth factor (VEGF) antisense oligodeoxynucleotide on mRNA and expression of VEGF, flt-1, and kinase insert domain containing receptor and VEGF excretion in human gallbladder carcinoma cells].
Zhonghua Yi Xue Za Zhi. 2007 Dec 18; 87(47):3329-34.ZY

Abstract

OBJECTIVE

To investigate the effects of vascular endothelial growth factor (VEGF) antisense oligodeoxynucleotide (ASODN) on the mRNA and protein expression of VEGF, Flt-1, and kinase insert domain containing receptor (KDR) and VEGF excretion in human gallbladder carcinoma cells.

METHODS

Human gallbladder carcinoma cells of the line GBC-SD were cultured and transfected with VEGF ASODN and sense oligodeoxynucleotide (SODN) mediated by Oligofectamine. The toxicity of SODN and Oligofectamine to the GBC-SD cells was examined by MTT method. RT-PCR was used to detect the mRNA and expression of VEGF, Flt-1, and KDR, and ELISA was used to detect the protein expression of VEGF.

RESULTS

MTT method showed that SODN and Oligofectamine were not toxic to the GBC-SD cells. The mRNA expression levels of VEGF, Flt-1, and KDR of the ASODN and ASODN + Oligofectamine groups were all significantly lower than those of the control group (all P < 0.05), and were the lowest 72 hours after transfection, and then gradually increased. ELISA showed that there were not significant differences in the VEGF protein concentration in the supernatant of the GBC-SD cells among the SODN, SODN + Oligofectamine, and control groups (all P < 0.05), however, the VEGF protein concentration in the supernatant of the GBC-SD cells of the ASDN and ASDN + Oligofectamine groups were significantly lower than that of the control group (both P < 0.05).

CONCLUSION

VEGF ASODN inhibits the mRNA and protein expression of VEGF, Flt-1, and KDR and VEGF excretion in human gallbladder carcinoma cells. Oligofectamine strengthens the effect of ASODN.

Authors+Show Affiliations

Department of Surgery, Second Affiliated Hospital, Medical School of Zhejiang University, Hangzhou 310009, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

18478945

Citation

Fang, He-Qing, et al. "[Effects of Vascular Endothelial Growth Factor (VEGF) Antisense Oligodeoxynucleotide On mRNA and Expression of VEGF, Flt-1, and Kinase Insert Domain Containing Receptor and VEGF Excretion in Human Gallbladder Carcinoma Cells]." Zhonghua Yi Xue Za Zhi, vol. 87, no. 47, 2007, pp. 3329-34.
Fang HQ, Li HJ, Liu YB, et al. [Effects of vascular endothelial growth factor (VEGF) antisense oligodeoxynucleotide on mRNA and expression of VEGF, flt-1, and kinase insert domain containing receptor and VEGF excretion in human gallbladder carcinoma cells]. Zhonghua Yi Xue Za Zhi. 2007;87(47):3329-34.
Fang, H. Q., Li, H. J., Liu, Y. B., Wang, X. A., Ma, X. M., Kong, Y., Chen, Y., Chen, D. Q., Weng, W. H., Zhang, Z. P., Devkota, K. R., Wang, J. W., Li, J. T., Cao, L. P., & Peng, S. Y. (2007). [Effects of vascular endothelial growth factor (VEGF) antisense oligodeoxynucleotide on mRNA and expression of VEGF, flt-1, and kinase insert domain containing receptor and VEGF excretion in human gallbladder carcinoma cells]. Zhonghua Yi Xue Za Zhi, 87(47), 3329-34.
Fang HQ, et al. [Effects of Vascular Endothelial Growth Factor (VEGF) Antisense Oligodeoxynucleotide On mRNA and Expression of VEGF, Flt-1, and Kinase Insert Domain Containing Receptor and VEGF Excretion in Human Gallbladder Carcinoma Cells]. Zhonghua Yi Xue Za Zhi. 2007 Dec 18;87(47):3329-34. PubMed PMID: 18478945.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of vascular endothelial growth factor (VEGF) antisense oligodeoxynucleotide on mRNA and expression of VEGF, flt-1, and kinase insert domain containing receptor and VEGF excretion in human gallbladder carcinoma cells]. AU - Fang,He-Qing, AU - Li,Hai-Jun, AU - Liu,Ying-Bin, AU - Wang,Xu-An, AU - Ma,Xiao-Ming, AU - Kong,Ying, AU - Chen,Yan, AU - Chen,De-Qin, AU - Weng,Wei-Hong, AU - Zhang,Zhi-Ping, AU - Devkota,K-R, AU - Wang,Jian-Wei, AU - Li,Jiang-Tao, AU - Cao,Li-Ping, AU - Peng,Shu-You, PY - 2008/5/16/pubmed PY - 2008/8/19/medline PY - 2008/5/16/entrez SP - 3329 EP - 34 JF - Zhonghua yi xue za zhi JO - Zhonghua Yi Xue Za Zhi VL - 87 IS - 47 N2 - OBJECTIVE: To investigate the effects of vascular endothelial growth factor (VEGF) antisense oligodeoxynucleotide (ASODN) on the mRNA and protein expression of VEGF, Flt-1, and kinase insert domain containing receptor (KDR) and VEGF excretion in human gallbladder carcinoma cells. METHODS: Human gallbladder carcinoma cells of the line GBC-SD were cultured and transfected with VEGF ASODN and sense oligodeoxynucleotide (SODN) mediated by Oligofectamine. The toxicity of SODN and Oligofectamine to the GBC-SD cells was examined by MTT method. RT-PCR was used to detect the mRNA and expression of VEGF, Flt-1, and KDR, and ELISA was used to detect the protein expression of VEGF. RESULTS: MTT method showed that SODN and Oligofectamine were not toxic to the GBC-SD cells. The mRNA expression levels of VEGF, Flt-1, and KDR of the ASODN and ASODN + Oligofectamine groups were all significantly lower than those of the control group (all P < 0.05), and were the lowest 72 hours after transfection, and then gradually increased. ELISA showed that there were not significant differences in the VEGF protein concentration in the supernatant of the GBC-SD cells among the SODN, SODN + Oligofectamine, and control groups (all P < 0.05), however, the VEGF protein concentration in the supernatant of the GBC-SD cells of the ASDN and ASDN + Oligofectamine groups were significantly lower than that of the control group (both P < 0.05). CONCLUSION: VEGF ASODN inhibits the mRNA and protein expression of VEGF, Flt-1, and KDR and VEGF excretion in human gallbladder carcinoma cells. Oligofectamine strengthens the effect of ASODN. SN - 0376-2491 UR - https://www.unboundmedicine.com/medline/citation/18478945/[Effects_of_vascular_endothelial_growth_factor__VEGF__antisense_oligodeoxynucleotide_on_mRNA_and_expression_of_VEGF_flt_1_and_kinase_insert_domain_containing_receptor_and_VEGF_excretion_in_human_gallbladder_carcinoma_cells]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&amp;issn=0376-2491&amp;year=2007&amp;vol=87&amp;issue=47&amp;fpage=3329 DB - PRIME DP - Unbound Medicine ER -