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Determination of tacrolimus in rabbit aqueous humor by liquid chromatography-electrospray ionization tandem mass spectrometry.
J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Jun 01; 868(1-2):34-41.JC

Abstract

A simple, sensitive and selective liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method was developed for the determination of tacrolimus (FK506) in rabbit aqueous humor. After a simple protein-precipitation by methanol, the post-treatment samples were separated on a reversed-phase, Thermo-Hypersil-BDS-C18 column with a mobile phase of a mixture of 0.1% formic acid in water, methanol and acetonitrile (5:85:10, v/v/v). Tacrolimus and ritonavir (internal standard, IS) were all detected by the selected reaction-monitoring (SRM) mode. The method developed was validated in rabbit aqueous humor with a daily working range of 0.5-100 ng/ml with correlation coefficient, r>0.99 and a sensitivity of 0.5 ng/ml as lower limit of quantification, respectively. This method was fully validated for the accuracy, precision, possible matrix effect and stability. The method proved to be accurate and specific, and was applied to the pharmacokinetic study of tacrolimus in rabbit aqueous humor.

Authors+Show Affiliations

Zhongshan Ophthalmic Center, Sun Yat-sen University, The State Key Laboratory of Ophthalmology, Guangzhou, Guangdong Province, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18479984

Citation

Yuan, Jin, et al. "Determination of Tacrolimus in Rabbit Aqueous Humor By Liquid Chromatography-electrospray Ionization Tandem Mass Spectrometry." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 868, no. 1-2, 2008, pp. 34-41.
Yuan J, Chen JQ, Xie ZY, et al. Determination of tacrolimus in rabbit aqueous humor by liquid chromatography-electrospray ionization tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2008;868(1-2):34-41.
Yuan, J., Chen, J. Q., Xie, Z. Y., Zhai, J. J., & Zhou, S. Y. (2008). Determination of tacrolimus in rabbit aqueous humor by liquid chromatography-electrospray ionization tandem mass spectrometry. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 868(1-2), 34-41. https://doi.org/10.1016/j.jchromb.2008.04.028
Yuan J, et al. Determination of Tacrolimus in Rabbit Aqueous Humor By Liquid Chromatography-electrospray Ionization Tandem Mass Spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Jun 1;868(1-2):34-41. PubMed PMID: 18479984.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of tacrolimus in rabbit aqueous humor by liquid chromatography-electrospray ionization tandem mass spectrometry. AU - Yuan,Jin, AU - Chen,Jia-Qi, AU - Xie,Zhi-Yong, AU - Zhai,Jia-Jie, AU - Zhou,Shi-You, Y1 - 2008/04/25/ PY - 2007/10/16/received PY - 2008/04/07/revised PY - 2008/04/11/accepted PY - 2008/5/16/pubmed PY - 2008/8/19/medline PY - 2008/5/16/entrez SP - 34 EP - 41 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 868 IS - 1-2 N2 - A simple, sensitive and selective liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method was developed for the determination of tacrolimus (FK506) in rabbit aqueous humor. After a simple protein-precipitation by methanol, the post-treatment samples were separated on a reversed-phase, Thermo-Hypersil-BDS-C18 column with a mobile phase of a mixture of 0.1% formic acid in water, methanol and acetonitrile (5:85:10, v/v/v). Tacrolimus and ritonavir (internal standard, IS) were all detected by the selected reaction-monitoring (SRM) mode. The method developed was validated in rabbit aqueous humor with a daily working range of 0.5-100 ng/ml with correlation coefficient, r>0.99 and a sensitivity of 0.5 ng/ml as lower limit of quantification, respectively. This method was fully validated for the accuracy, precision, possible matrix effect and stability. The method proved to be accurate and specific, and was applied to the pharmacokinetic study of tacrolimus in rabbit aqueous humor. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/18479984/Determination_of_tacrolimus_in_rabbit_aqueous_humor_by_liquid_chromatography_electrospray_ionization_tandem_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(08)00240-7 DB - PRIME DP - Unbound Medicine ER -